Genom‐wide analysis identifies single nucleotide polymorphism variations and altered pathways associated with poor semen quality in breeding bulls

King, John Peter Ebenezer Samuel; Kumaresan, A.; Sinha, Manish Kumar; Raval, Kathan; Nag, Pranab Kumar; Karuthadurai, Thirumalaisamy; Aranganathan, V.

doi:10.1111/rda.14185

Cited by 5 publications

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“…The MAPK signalling pathway is involved in sperm development and function through spermatogenesis and fertilising potential. In mature spermatozoa, the MAPK signalling pathway plays an important role in acrosome reaction, hyperactivation, and motility ( Almog and Naor, 2010 ; Ebenezer Samuel King et al, 2022 ). We also observed the downregulation of cytoskeletal protein binding pathways in the cryopreserved sample.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation process alters the expression of genes involved in pathways associated with the fertility of bull spermatozoa

et al. 2022

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In bovines, cryopreserved semen is used for artificial insemination; however, the fertility of cryopreserved semen is far lower than that of fresh semen. Although cryopreservation alters sperm phenotypic characteristics, its effect on sperm molecular health is not thoroughly understood. The present study applied next-generation sequencing to investigate the effect of cryopreservation on the sperm transcriptomic composition of bull spermatozoa. While freshly ejaculated bull spermatozoa showed 14,280 transcripts, cryopreserved spermatozoa showed only 12,375 transcripts. Comparative analysis revealed that 241 genes were upregulated, 662 genes were downregulated, and 215 genes showed neutral expression in cryopreserved spermatozoa compared to fresh spermatozoa. Gene ontology analysis indicated that the dysregulated transcripts were involved in nucleic acid binding, transcription-specific activity, and protein kinase binding involving protein autophosphorylation, ventricular septum morphogenesis, and organ development. Moreover, the dysregulated genes in cryopreserved spermatozoa were involved in pathways associated with glycogen metabolism, MAPK signalling, embryonic organ morphogenesis, ectodermal placode formation, and regulation of protein auto-phosphorylation. These findings suggest that the cryopreservation process induced alterations in the abundance of sperm transcripts related to potential fertility-associated functions and pathways, which might partly explain the reduced fertility observed with cryopreserved bull spermatozoa.

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Section: Discussionmentioning

confidence: 99%

Cryopreservation process alters the expression of genes involved in pathways associated with the fertility of bull spermatozoa

et al. 2022

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Cross-ancestry meta-genome-wide association studies provide insights to the understanding of semen traits in pigs

Cheng,

Zhang,

Han

et al. 2024

animal

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Determinant genetic markers of semen quality in livestock

Khan,

Chen,

Naz

et al. 2024

Front. Endocrinol.

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The reproductive efficiency of livestock is crucial for agricultural productivity and economic sustainability. One critical factor in successful fertilization and the viability of offspring is the quality of semen. Poor semen quality, especially in frozen-thawed semen used in artificial insemination (AI) have been shown to influence conception outcomes, resulting a negative impact on livestock production. Recent advancements in genetic research have identified specific markers linked to semen quality traits in various livestock species, such as cattle, sheep, goats, pigs, buffalo, and equines. These genetic markers are essential in screening males for breeding suitability, which in turn enhances selective breeding programs. Understanding these markers is crucial for improving reproductive performance and increasing productivity in livestock populations. This review offers a comprehensive overview of the genetic markers associated with semen quality in key livestock. It explores the underlying genetic mechanisms and their practical implications in animal breeding and management. The review underscores the importance of integrating genetic insights into breeding strategies to optimize reproductive efficiency and ensure the sustainable development of livestock industries.

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Genom‐wide analysis identifies single nucleotide polymorphism variations and altered pathways associated with poor semen quality in breeding bulls

Cited by 5 publications

References 47 publications

Cryopreservation process alters the expression of genes involved in pathways associated with the fertility of bull spermatozoa

Cryopreservation process alters the expression of genes involved in pathways associated with the fertility of bull spermatozoa

Cross-ancestry meta-genome-wide association studies provide insights to the understanding of semen traits in pigs

Determinant genetic markers of semen quality in livestock

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