Genome analysis of two type 6 echovirus (E6) strains recovered from sewage specimens in Greece in 2006

Kyriakopoulou, Zaharoula; Pliaka, Vaia; Tsakogiannis, D.; Ruether, I. G. A.; Komiotis, Dimitris; Gartzonika, Constantina; Levidiotou-Stefanou, Stamatina; Markoulatos, Panayotis

doi:10.1007/s11262-011-0688-3

Cited by 10 publications

(4 citation statements)

References 48 publications

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“…In previous studies, many serotypes of EV were reported of causing encephalitis outbreak in children, such as Echovirus 30 in Belgium, 2000, [ 20 ] Echovirus 6 in Greece, 2006, [ 21 ] Echovirus 4 in Spain, 2008, [ 22 ] Echovirus 19 in Uttar Pradesh, India, 2008, [ 23 ] Echovirus 9 in Sri Lanka, 2009, [ 24 ] and Coxsackievirus B5 in Henan, China [ 25 ] ; Echovirus 30 is a mostly common serotype in meningitis outbreaks caused by EV around the world. [ 26 ] According to the sequence of VP1 or 5′UTR, Echovirus 30 was found to be the major serotype in Hangzhou, 2015.…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiological study of enteroviruses associated with encephalitis in children from Hangzhou, China

Zhang²,

Xiao-ting³

et al. 2016

Medicine

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Enterovirus (EV) has over 100 serotypes of species A–D, which can cause various symptoms in infants. Enterovirus encephalitis (EVE) is serve disease with high morbidity and mortality in children. To well define the epidemiology of EVE, we wanted to know more about EV and EV molecular typing by conducting this study in Hangzhou.Cerebrospinal fluid samples were collected from children with diagnosis of encephalitis. Meanwhile, one-step real-time RT-PCR was used for the detection of EV, and we also identified the serotypes of EV by using gene sequencing of VP1 or 5′UTR region.A total of 126 CSF specimens were tested and EV was detected in 26 specimens (20.6%). The molecular typing results showed different types of EV strains including Coxsackievirus B2, Coxsackievirus B3, Echovirus 5, Echovirus 16, Echovirus 18, Echovirus 30, and all EV isolates belonging to the human EV species B.According to the sequence of VP1 and 5′UTR region, E30 may be major cause of children's EVE in Hangzhou, China.

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Section: Discussionmentioning

confidence: 99%

Molecular epidemiological study of enteroviruses associated with encephalitis in children from Hangzhou, China

Zhang²,

Xiao-ting³

et al. 2016

Medicine

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“…The RF groups assigned were distinct from 3Dpol sequence groupings of the prototype Amori and Charles E6 isolates (AY302558 and AF465517), the recently described E6 variants in the C9c lineage associated with an outbreak of aseptic meningitis in Japan [AB705308–AB705311; Miyoshi et al, ] and the C7 lineage sequences from Greece [Kyriakopoulou et al, ]. 3Dpol sequences from E6 variants in the latter two studies fell into clades distinct from those of other E6 or species B serotypes.…”

Section: Resultsmentioning

confidence: 84%

Recombination and evolutionary dynamics of human echovirus 6

Cabrerizo

Trallero

Simmonds

2013

Journal of Medical Virology

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Enterovirus (EV) infections are associated with a wide array of often severe disease presentations including aseptic meningitis, encephalitis, and acute flaccid paralysis. Surveillance for polioviruses and other EVs is therefore important as a public health measure both for patient management and epidemiological studies. From 1988 to 2008, echovirus (E) 30 was the predominant genotype in Spain (33.7% of the total typed EVs). E6 was also endemic throughout this period although isolated less frequently (12.5%). In 2009, however, a substantial increase in the incidence of E6 was detected (60%), displacing E30 type (2%). To investigate the evolution and recombination in the epidemiology and transmission of E6 in Spain, a genetic analysis in VP1 and 3Dpol regions of 67 Spanish strains collected during the period 2004-2010 was performed. All VP1 sequences clustered monophyletically in the assigned genogroup C, subgroup 9, currently the predominant circulating strains identified in Europe and elsewhere in the last 10 years. 3Dpol sequences were interspersed with other species B EVs resulting from several recombination events that generated at least 12 different recombinant forms (RFs) among study samples. These showed typically minimal divergence in VP1. The co-circulation of different lineages of E6 in the same geographical area associated with its mainly endemic pattern of transmission may have contributed to the extremely short estimated half-life of E6 RFs (0.87 years). This pattern contrasts markedly with other species B EVs and EV71 where VP1 lineage expansion and extinction occurred in step with defined recombination events and periodic changes in incidence.

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“…In previous studies, many serotypes of EV were reported of causing encephalitis outbreak in children, such as Echovirus 30 in Belgium, 2000, [20] Echovirus 6 in Greece, 2006, [21] Echovirus 4 in Spain, 2008, [22] Echovirus 19 in Uttar Pradesh, India, 2008, [23] Echovirus 9 in Sri Lanka, 2009, [24] and Coxsackievirus B5 in Henan, China [25] ; Echovirus 30 is a mostly common serotype in meningitis outbreaks caused by EV around the world. [26] According to the sequence of VP1 or 5 0 UTR, Echovirus 30 was found to be the major serotype in Hangzhou, 2015.…”

Section: Eve (Average)mentioning

confidence: 99%

Molecular epidemiological study of enteroviruses associated with encephalitis in children from Hangzhou, China

2016

Medicine

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Enterovirus (EV) has over 100 serotypes of species A-D, which can cause various symptoms in infants. Enterovirus encephalitis (EVE) is serve disease with high morbidity and mortality in children. To well define the epidemiology of EVE, we wanted to know more about EV and EV molecular typing by conducting this study in Hangzhou.Cerebrospinal fluid samples were collected from children with diagnosis of encephalitis. Meanwhile, one-step real-time RT-PCR was used for the detection of EV, and we also identified the serotypes of EV by using gene sequencing of VP1 or 5 0 UTR region. A total of 126 CSF specimens were tested and EV was detected in 26 specimens (20.6%). The molecular typing results showed different types of EV strains including Coxsackievirus B2, Coxsackievirus B3, Echovirus 5, Echovirus 16, Echovirus 18, Echovirus 30, and all EV isolates belonging to the human EV species B.According to the sequence of VP1 and 5 0 UTR region, E30 may be major cause of children's EVE in Hangzhou, China.Abbreviations: CSF = cerebrospinal fluid, EV = enterovirus, EVE = enterovirus encephalitis, GLU = glucose, RT-PCR = reverse transcription-polymerase chain reaction.

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Genome analysis of two type 6 echovirus (E6) strains recovered from sewage specimens in Greece in 2006

Cited by 10 publications

References 48 publications

Molecular epidemiological study of enteroviruses associated with encephalitis in children from Hangzhou, China

Molecular epidemiological study of enteroviruses associated with encephalitis in children from Hangzhou, China

Recombination and evolutionary dynamics of human echovirus 6

Molecular epidemiological study of enteroviruses associated with encephalitis in children from Hangzhou, China

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