Genome complexity of a powerful cellulolytic fungus,Penicillium funiculosum

Abstract

The genomic complexity of a powerful cellulolytic fungus, Penicillium funiculosum was investigated by studying its DNA reassociation kinetics. Approx. 10% of the total genomic DNA formed duplexes 5828 times faster than the slow component and these were considered to consist of repeated DNA sequences. The latter exhibited a high degree of sequence homogeneity and comprised long as well as short stretches of repeated sequences. The slow component which mainly represented the single copy DNA sequences had a kinetic complexity of 28.7 × 109 Da.