Genome editing reveals dmrt1 as an essential male sex-determining gene in Chinese tongue sole (Cynoglossus semilaevis)

Cui, Zhongkai; Liu, Yun; Wang, Wenwen; Wang, Qian; Zhang, Ning; Lin, Fan; Wang, Na; Shao, Changwei; Dong, Zhongdian; Li, Yangzhen; Yang, Yingming; Hu, Mengzhu; Li, Hailong; Gao, Fengtao; Wei, Zhanfei; Meng, Liang; Liu, Yang; Wei, Min; Zhu, Ying; Guo, Hua; Chen, Cheng; Schartl, Manfred; Chen, Songlin

doi:10.1038/srep42213

Cited by 157 publications

(94 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Such technologies as zinc-finger nucleases, transcription activator-like effector nucleases and clustered, regularly interspaced, short palindromic repeats (CRISPR)-CRISPR-associated (Cas) systems (CRISPR/Cas9) have emerged as new tools for potential improvement of aquacultured fish (Cui et al, 2017;Li and Wang, 2017). The CRISPR/Cas9 gene editing technology has been used to generate lines of site directed mutated cell lines of Chinook salmon O. tshawytscha (Dehler et al, 2016) and can be used for determination of SNP function and QTL assessment (Macqueen et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Single nucleotide polymorphism markers with applications in aquaculture and assessment of its impact on natural populations

Wenne

2017

Aquat. Living Resour.

View full text Add to dashboard Cite

-An increase in aquatic animal production can be achieved by extending aquaculture areas geographically, utilizing new species for culture, and using new technologies. Among new technologies useful for the increase of aquaculture production is the application of genetics and genomics. New molecular tools that benefit aquaculture have been developed. There has been a large number of experimental and review papers published concerning molecular markers and the range of their applications, including aquaculture and food product analyses. Analysis of single nucleotide polymorphisms (SNPs) has emerged as genotyping technology with wide and significant applications in aquaculture. SNPs can be used for construction of genetic linkage maps, finding quantitative trait loci (QTL) for useful traits like growth, body weight, grilsing, thermal and low oxygen tolerance, resistance to stress and diseases, mapping sex determination loci and identification of progeny in selection and chromosome manipulation experiments, assessment of genomic selectionand marker assisted selection in aquaculture. Genome-wide association studies (GWAS) facilitate the finding associations between SNPs and a trait in related or unrelated specimens. However, many traits are complex and can be controlled by number of QTL. Genotyping by genome reduction complexity sequencing emerged as an efficient and applicable technology in genomic selection. Identification of genes, sequences and nucleotides (substitutions) directly influencing phenotypic variations opens the possibility of marker-assisted selection for desirable characters in culture. SNP and QTL associations can be enhanced using genome editing technology. Examples of successful applications of SNPs in aquaculture of fish, crustacean and mollusk species, representing most geographic areas, and ecological risks assessment are reviewed.

show abstract

Section: Discussionmentioning

confidence: 99%

Single nucleotide polymorphism markers with applications in aquaculture and assessment of its impact on natural populations

Wenne

2017

Aquat. Living Resour.

View full text Add to dashboard Cite

show abstract

“…semilaevis [2]. In the gonads of DMRT1 -knock out fish, no secondary spermatocytes, spermatids and sperms have been detected, and only spermatogonia and primary spermatocytes were found [5]. In addition, ZZZ triploid males cannot produce normal sperm due to the inhibition of testis development [4].…”

Section: Discussionmentioning

confidence: 99%

“…Triploid males were produced using hydraulic pressure shock, and triploidy was validated by karyotyping and flow cytometry analysis [3]. DMRT1 -knock out fish was also produced in our laboratory by microinjecting the TALEN mRNAs targeted towards dmrt1 into sole embryos (wild-type) [5]. Total RNA was extracted with a TRIzol isolation kit (Qiagen, Dusseldorf, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…However, artificial ZZZ triploid males of tongue sole cannot produce normal sperm due to the inhibition of testis development [3–4]. Furthermore, DMRT1 is a vital gene that is involved in the male sex-determination of tongue sole [2, 5], and the spermatogenesis process was disrupted in the ZZ dmrt1 mutant tongue sole. Compared to wild-type males, only a few spermatogonia, and few or in some cases even no spermatocytes and spermatids were observed [5].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, DMRT1 is a vital gene that is involved in the male sex-determination of tongue sole [2, 5], and the spermatogenesis process was disrupted in the ZZ dmrt1 mutant tongue sole. Compared to wild-type males, only a few spermatogonia, and few or in some cases even no spermatocytes and spermatids were observed [5]. Although the whole genomic sequence of C .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular cloning and expression analysis of the aqp1aa gene in half-smooth tongue sole (Cynoglossus semilaevis)

et al. 2017

Self Cite

View full text Add to dashboard Cite

Aquaporin 1 (AQP1) is a member of the transmembrane water channel family of proteins with special structural features, and two AQP1 paralogous genes (aqp1aa and aqp1ab) are reported in teleosts. In the present study, the aqp1aa gene of half-smooth tongue sole (Cynoglossus semilaevis) was cloned and characterized. The full-length cDNA of aqp1aa is 1411 bp with a 786 bp open reading frame encoding a 261-amino acid putative protein with a characteristic structure consisting of 6 membrane-spanning α-helical domains and two highly conserved asparagine—proline—alanine motifs. Real-time quantitative PCR revealed that aqp1aa mRNA is expressed predominantly in the testis of males and pseudo-males, while its expression is low in the ovary and lowest in doublesex and mab-3-related transcription factor 1(DMRT1) knock out fish and triploid males. In situ hybridization indicated that aqp1aa mRNA is expressed mainly in the germ cells of males and pseudo-males, especially in spermatozoa and spermatids. These results suggest that the aqp1aa may play a role in spermatogenesis of C. semilaevis.

show abstract

Genomic and Epigenetic Aspects of Sex Determination in Half‐Smooth Tongue Sole

Chen

Zhou

Shao

2018

Sex Control in Aquaculture

View full text Add to dashboard Cite

Genome editing reveals dmrt1 as an essential male sex-determining gene in Chinese tongue sole (Cynoglossus semilaevis)

Cited by 157 publications

References 44 publications

Single nucleotide polymorphism markers with applications in aquaculture and assessment of its impact on natural populations

Single nucleotide polymorphism markers with applications in aquaculture and assessment of its impact on natural populations

Molecular cloning and expression analysis of the aqp1aa gene in half-smooth tongue sole (Cynoglossus semilaevis)

Genomic and Epigenetic Aspects of Sex Determination in Half‐Smooth Tongue Sole

Contact Info

Product

Resources

About