Genome Editing: Revolutionizing the Crop Improvement

Saurabh, Satyajit

doi:10.1007/s11105-021-01286-7

Cited by 20 publications

(7 citation statements)

References 175 publications

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“…The in vivo generation of haploid seeds particularly with the development and use of HIS would be able to transform the rice improvement programme, particularly in the indica cultivars due to their comfy nature over in vitro methods (Dwivedi et al, 2015; Saurabh, 2021; Watts et al, 2018). Although the in vivo HI system using HIS was reported in maize even before the in vitro‐based methods of haploid production, it could not be exploited in other agronomical important crops due to the unknown mechanism of HI.…”

Section: Methods Of Himentioning

confidence: 99%

Strategies and prospects of haploid induction in rice (Oryza sativa)

et al. 2021

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Being a model crop as well as a staple food crop, rice possesses a conspicuous position in plant sciences. Doubled haploid (DH) breeding in rice offers multiple advantages over conventional methods via accelerating the process of pure lines development. For ages, several methods have been devised for haploid production in rice. In vitro‐based methods have demonstrated their potential in DH production, but these methods are further impeded by stringent requirements and restricted to few species. Further, unlike maize, the in vivo DH breeding technology is still impractical in rice. Recent findings of the key molecular players behind the haploid induction (HI) trait in maize and rice have shown their potential in rice improvement programmes. In this review, we have discussed different strategies of HI in rice particularly focusing on recent progress made for developing haploid inducer stock (HIS) by employing both conventional and modern approaches. It also focuses on future implications of HIS so that it can be incorporated in the pipeline of modernized rice breeding.

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Section: Methods Of Himentioning

confidence: 99%

Strategies and prospects of haploid induction in rice (Oryza sativa)

et al. 2021

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“…These proteins imitate transcription factors once they attach to the DNA sequence and can control the activation of target gene(s) (Becker and Boch. 2021;Saurabh, 2021). Researchers decided to use it as a tool for gene editing by creating two hybrid TALE nucleases, each containing a DBD and the catalytic domain FokI.…”

Section: Technical Prelude To Evolution Of Ge Technologiesmentioning

confidence: 99%

A CRISPR way for accelerating cereal crop improvement: Progress and challenges

Basu

Ahmed²,

Bhat³

et al. 2023

Front. Genet.

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Humans rely heavily on cereal grains as a key source of nutrients, hence regular improvement of cereal crops is essential for ensuring food security. The current food crisis at the global level is due to the rising population and harsh climatic conditions which prompts scientists to develop smart resilient cereal crops to attain food security. Cereal crop improvement in the past generally depended on imprecise methods like random mutagenesis and conventional genetic recombination which results in high off targeting risks. In this context, we have witnessed the application of targeted mutagenesis using versatile CRISPR-Cas systems for cereal crop improvement in sustainable agriculture. Accelerated crop improvement using molecular breeding methods based on CRISPR-Cas genome editing (GE) is an unprecedented tool for plant biotechnology and agriculture. The last decade has shown the fidelity, accuracy, low levels of off-target effects, and the high efficacy of CRISPR technology to induce targeted mutagenesis for the improvement of cereal crops such as wheat, rice, maize, barley, and millets. Since the genomic databases of these cereal crops are available, several modifications using GE technologies have been performed to attain desirable results. This review provides a brief overview of GE technologies and includes an elaborate account of the mechanisms and applications of CRISPR-Cas editing systems to induce targeted mutagenesis in cereal crops for improving the desired traits. Further, we describe recent developments in CRISPR-Cas–based targeted mutagenesis through base editing and prime editing to develop resilient cereal crop plants, possibly providing new dimensions in the field of cereal crop genome editing.

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“…This platform, for which a Nobel prize has been awarded, is now widely used in various disease models, including inherited gene defects and cancers [102]. It is also applied in disease detection and diagnosis, genetically modified foods, and farming [103,104]. CRISPR gene editing technology has been applied in research on Chinese medicine compound formulas.…”

Section: Crispr Gene Editingmentioning

confidence: 99%

Multi-component Chinese medicine formulas for drug discovery: State of the art and future perspectives

Zhang

Chen

Tang

et al. 2023

Acta Materia Medica

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For hundreds of years, the drug discovery and development industry has aimed at identifying single components with a clear mechanism of action as desirable candidates for potential drugs. However, this conventional strategy of drug discovery and development has faced challenges including a low success rate and high development costs. Herein, we critically review state-of-the-art drug discovery and development based on multi-component Chinese medicine formulas. We review the policies and application status of new drugs based on multi-component Chinese medicines in the US, China, and the European Union. Moreover, we illustrate several excellent cases of ongoing applications. Biomedical technologies that may facilitate drug discovery and development based on multi-component Chinese medicine formulas are discussed, including network pharmacology, integrative omics, CRISPR gene editing, and chemometrics. Finally, we discuss potential problems and solutions in pre-clinical and clinical research in drug discovery and development based on multi-component Chinese medicine formulas. We hope that this review will promote discussion of the roles of multi-component Chinese medicine formulas in the discovery and development of new drugs for the treatment of human diseases.

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Genome Editing: Revolutionizing the Crop Improvement

Cited by 20 publications

References 175 publications

Strategies and prospects of haploid induction in rice (Oryza sativa)

Strategies and prospects of haploid induction in rice (Oryza sativa)

A CRISPR way for accelerating cereal crop improvement: Progress and challenges

Multi-component Chinese medicine formulas for drug discovery: State of the art and future perspectives

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