Genome engineering in insects for the control of vector borne diseases

Hillary, V. Edwin; Ceasar, S. Antony

doi:10.1016/bs.pmbts.2020.12.017

Cited by 8 publications

(6 citation statements)

References 100 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using effector fusion (Cas9 proteins or sgRNA) to alter transcription states of specific genomic loci, or rearrange the genome, can significantly expand the genome engineering modification utilizing the CRISPR/Cas9 system. CRISPR/Cas9 system has been widely adopted by researchers and applied in diverse fields, including microbes [ 48 ], plants [ 49 – 51 ], animals [ 52 ], insects [ 53 , 54 ], and human cell lines [ 55 ] (briefly explained in Fig. 3 ).…”

Section: Cas9 Proteinmentioning

confidence: 99%

A Review on the Mechanism and Applications of CRISPR/Cas9/Cas12/Cas13/Cas14 Proteins Utilized for Genome Engineering

2022

Self Cite

View full text Add to dashboard Cite

The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (CRISPR/Cas) system has altered life science research offering enormous options in manipulating, detecting, imaging, and annotating specific DNA or RNA sequences of diverse organisms. This system incorporates fragments of foreign DNA (spacers) into CRISPR cassettes, which are further transcribed into the CRISPR arrays and then processed to make guide RNA (gRNA). The CRISPR arrays are genes that encode Cas proteins. Cas proteins provide the enzymatic machinery required for acquiring new spacers targeting invading elements. Due to programmable sequence specificity, numerous Cas proteins such as Cas9, Cas12, Cas13, and Cas14 have been exploited to develop new tools for genome engineering. Cas variants stimulated genetic research and propelled the CRISPR/Cas tool for manipulating and editing nucleic acid sequences of living cells of diverse organisms. This review aims to provide detail on two classes (class 1 and 2) of the CRISPR/Cas system, and the mechanisms of all Cas proteins, including Cas12, Cas13, and Cas14 discovered so far. In addition, we also discuss the pros and cons and recent applications of various Cas proteins in diverse fields, including those used to detect viruses like severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). This review enables the researcher to gain knowledge on various Cas proteins and their applications, which have the potential to be used in next-generation precise genome engineering.

show abstract

Section: Cas9 Proteinmentioning

confidence: 99%

A Review on the Mechanism and Applications of CRISPR/Cas9/Cas12/Cas13/Cas14 Proteins Utilized for Genome Engineering

2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…These two subfamilies pose a severe threat to humans and animals because of their widespread occurrence. They transmit diseases like dengue, chikungunya, zika, yellow fever, malaria, Japanese encephalitis, and filariasis (Gabrieli et al, 2014;Bird & McElroy, 2016;Benelli & Duggan, 2018;Hillary & Ceasar, 2021). These mosquito-borne diseases endanger people in tropical and subtropical areas, confirming that half of the world's population is at higher risk (WHO, 2017(WHO, , 2022a.…”

Section: Introductionmentioning

confidence: 99%

“…Mosquitoes (Diptera: Culicidae) are major arthropod groups that existed over 180–220 million years ago (Gabrieli et al., 2014; Bird & McElroy, 2016; Benelli & Duggan, 2018; Hillary & Ceasar, 2021). Mosquitoes belong to two subfamilies (Gabrieli et al., 2014): Anophelinae ( Anopheles ) and Culicinae ( Aedes , Culex , and Mansonia ).…”

Section: Introductionmentioning

confidence: 99%

Efficacy of plant products in controlling disease vector mosquitoes, a review

Hillary,

Ceasar,

Ignacimuthu

2024

Entomologia Exp Applicata

Self Cite

View full text Add to dashboard Cite

Mosquitoes pose a severe threat to the environment as vectors of numerous harmful diseases affecting humans and animals. Mosquitoes transmit pathogens that cause dengue, chikungunya, malaria, zika, yellow fever, Japanese encephalitis, and filariasis. Today's mosquito control strategies heavily rely on the use of chemical insecticides such as N,N‐diethyl‐meta‐toluamide (DEET), N,N‐diethyl mandelic acid amide (DEM), and dimethyl phthalate (DMP). However, the widespread use of chemical insecticides has resulted in pollution, bio‐magnification, and other health and environmental issues. It has also become ineffective because of the mosquitoes' aptitude to develop resistance, emphasizing the urgent need for safe, effective, and long‐lasting strategies. An alternative and promising approach to circumventing these obstacles involves the implementation of insecticides made from natural compounds found in plants. Therefore, the scientific community has shifted its focus towards plant‐based phytochemicals, oils, and extracts, as these are eco‐friendly, safe, and cost‐effective alternatives to conventional chemical insecticides. This review aims to provide details on current advances in plant‐based products (plant compounds, extracts, and essential oils), which are used to control all the life cycle stages (egg, larva, pupa, and adult) of the mosquito genera Aedes, Anopheles, and Culex. Hopefully, this review will pave the way to devise control strategies against these challenging pests.

show abstract

“…Bite of these mosquitoes causes allergic responses with high fever, rash, nausea, limb swelling, and sometimes bleeding and death. Therefore, real vector control approaches are needed to avoid mosquito-borne diseases and improve community health (Hillary and Ceasar 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Globally, every year, more than 1 billion cases and 1 million deaths are recorded due to these mosquito-borne infectious diseases. Mosquitoes belonging to the genera Aedes, Anopheles, and Culex transmit dengue, chikungunya, yellow fever, zika, malaria, and lariasis to humans (Hillary and Ceasar 2021). Bite of these mosquitoes causes allergic responses with high fever, rash, nausea, limb swelling, and sometimes bleeding and death.…”

Section: Introductionmentioning

confidence: 99%

Effect of (4R, 5S) 4-hydroxy-7-angeloyloxycarvatoneacetone from Blumea axillaris (Lam.) DC. plant against An. stephensi and Cx. quinquefasciatus vector mosquitoes (Diptera: Culicidae) and expression of different functional genes

Hillary

Sivanandhan

Ceasar

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

The present study was conducted to evaluate the mosquitocidal efficiency using a compound isolated from Blumea axillaris (Lam.) DC. against Culex quinquefasciatus and Anopheles stephensi. The compound isolated from leaves of B. axillaris is (4R, 5S) 4-hydroxy-7-angeloyloxycarvatoneacetone. This isolated compound was exposed to eggs and larvae of An. stephensi and Cx. quinquefasciatus and with different concentrations of 0.5, 1.0, 1.5, and 2.0 ppm. Primarily, the hexane extract of an isolated compound exhibited promising larvicidal activities of LC50 and LC90 values of 155.811 and 566.763 ppm against An. stephensi and 77.215 and 99.653 against Cx. quinquefasciatus at 24 hours. Secondly, the compound isolated from B. axillaris at 2-ppm concentration exhibited LC50 and LC90 values of 0.85 and 3.59 and 1.19 and 3.678 ppm against Cx. quinquefasciatus and An. stephensi larvae, respectively. Furthermore, histopathological studies revealed serious damage to the larval midgut cells in both Cx. quinquefasciatus and An. stephensi (treated with compound). Additionally, it showed 81.0% and 84.2% ovicidal activity against both Cx. quinquefasciatus and An. stephensi eggs at 120 h post-treatment. In addition, we also analyzed the influence of isolated compound on the expression of different functional genes of An. stephensi and Cx. quinquefasciatus namely (1) White (Wh) gene, which is responsible for eye pigmentation; (2) the Glutathione transferase (GST) gene that helps for survival when exposed to any insecticides or pesticides; (3) Cytochrome P450 monooxygenase (CYP450) gene that enhances resistance in mosquitoes to digest pesticides and (4) Esterase (Est) gene, which helps in resistance to the variety of insecticides. These overall studies and results clearly suggest that compounds from B. axillaris could act as good mosquitocidal agents against both Cx. quinquefasciatus and An. stephensi and the compound were also first time reported as mosquitocidal activity.

show abstract

Genome engineering in insects for the control of vector borne diseases

Cited by 8 publications

References 100 publications

A Review on the Mechanism and Applications of CRISPR/Cas9/Cas12/Cas13/Cas14 Proteins Utilized for Genome Engineering

A Review on the Mechanism and Applications of CRISPR/Cas9/Cas12/Cas13/Cas14 Proteins Utilized for Genome Engineering

Efficacy of plant products in controlling disease vector mosquitoes, a review

Effect of (4R, 5S) 4-hydroxy-7-angeloyloxycarvatoneacetone from Blumea axillaris (Lam.) DC. plant against An. stephensi and Cx. quinquefasciatus vector mosquitoes (Diptera: Culicidae) and expression of different functional genes

Contact Info

Product

Resources

About