Genome-guided purification of high amounts of the siderophore ornibactin and detection of potentially novel burkholdine derivatives produced by Burkholderia catarinensis 89T

Abstract

Aim The increased availability of genome sequences has enabled the development of valuable tools for the prediction and identification of bacterial natural products. Burkholderia catarinensis 89T produces siderophores and an unknown potent antifungal metabolite. The aim of this work was to identify and purify natural products of B. catarinensis 89T through a genome-guided approach. Materials and methods The analysis of B. catarinensis 89T genome revealed 16 clusters putatively related to secondary metabolism and antibiotics production. Of particular note was the identification of an NRPS cluster related to the production of the siderophore ornibactin, a hybrid NRPS- PKS Type 1 cluster for the production of the antifungal glycolipopeptide burkholdine, and a gene cluster encoding homoserine lactones (HSL), probably involved in the regulation of both metabolites. We were able to purify high amounts of the ornibactin derivatives D/C6 and F/C8, while also detecting the derivative B/C4 in mass spectrometry investigations. A group of metabolites with molecular masses ranging from 1188 to 1272 Da could be detected in MS experiments, which we postulate to be new burkholdine analogs produced by B. catarinensis. The comparison of B. catarinensis BGCs with other Bcc members corroborates the hypothesis that this bacterium could produce new derivatives of these metabolites. Moreover, the quorum sensing metabolites C6-HSL, C8-HSL, and 3OH-C8-HSL were observed in LC-MS/MS analysis. Conclusion and impact of the study The new species Burkholderia catarinensis is a potential source of new bioactive secondary metabolites. Our results highlight the importance of genome-guided purification and identification of metabolites of biotechnological importance.

A Practical Toolkit for the Detection, Isolation, Quantification, and Characterization of Siderophores and Metallophores in Microorganisms

“…PlantiSMASH, a branch of antiSMASH, incorporates plant-specific functionality, including plant-adapted hidden Markov model profiles and cluster detection logic, and supports coexpression analysis . An interesting study reported the genome-guided purification of high amounts of the siderophore ornibactin and the detection of potentially novel burkholdine derivatives produced by Burkholderia catarinensis 89T . In that study, the authors, by using genome-guided purification protocols followed by mass spectrometry techniques, were able to detect masses related to putative new derivatives of the siderophore ornibactin and the antifungal burkholdine as well as homoserine lactones probably related to their regulation .…”

“… 122 An interesting study reported the genome-guided purification of high amounts of the siderophore ornibactin and the detection of potentially novel burkholdine derivatives produced by Burkholderia catarinensis 89T. 126 In that study, the authors, by using genome-guided purification protocols followed by mass spectrometry techniques, were able to detect masses related to putative new derivatives of the siderophore ornibactin and the antifungal burkholdine as well as homoserine lactones probably related to their regulation. 126 The antiSMASH technology was used for the characterization of siderophores from E. coli to find the secondary metabolites that cause pathogenicity in urinary tract infections (UTIs).…”

“… 126 In that study, the authors, by using genome-guided purification protocols followed by mass spectrometry techniques, were able to detect masses related to putative new derivatives of the siderophore ornibactin and the antifungal burkholdine as well as homoserine lactones probably related to their regulation. 126 The antiSMASH technology was used for the characterization of siderophores from E. coli to find the secondary metabolites that cause pathogenicity in urinary tract infections (UTIs). 127 Since UTIs happen when exogenous and endogenous bacteria enter the urinary tract and colonize there, the discovery of secondary metabolites that cause pathogenicity can be used for the development of future pharmaceutical approaches and drugs.…”