Genome sequence analysis and bioactivity profiling of marine-derived actinobacteria, Brevibacterium luteolum, and Cellulosimicrobium funkei

Tanveer, Faouzia; Shehroz, Muhammad; Ali, Muhammad; Xie, Yunying; Abbasi, Rashda; Yasmin, Azra

doi:10.1007/s00203-021-02203-y

Cited by 5 publications

(4 citation statements)

References 50 publications

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“…The presented tree incorporates type strains from other genera beyond Cellulosimicrobium , enabling a more comprehensive exploration of its evolutionary relationships among other closely related Actinomycetota taxa. C. funkei, formerly Oerskovia turbata , was firstly isolated from a human blood sample, however strains affiliated with this species have since been isolated from various other settings, including the marine environment [ 26 ].…”

Section: Resultsmentioning

confidence: 99%

“…While some exist as free-living entities, others establish symbiotic relationships with several organisms, such as macroalgae; these associations prompt the synthesis of NPs with tailored bioactive properties [ 23 ]. Cellulosimicrobium strains have been retrieved from the marine environment, including as epiphytes of macroalgae [ 24 ], and recent studies have shown their antifungal and antioxidant properties [ 25 , 26 ]. However, to our knowledge, no NP to date has been described from the secondary metabolism of a Cellulosimicrobium strain.…”

Section: Introductionmentioning

confidence: 99%

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Cellulamides: A New Family of Marine-Sourced Linear Peptides from the Underexplored Cellulosimicrobium Genus

Girão,

Murillo-Alba,

Martín

et al. 2024

Marine Drugs

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Bioprospecting the secondary metabolism of underexplored Actinomycetota taxa is a prolific route to uncover novel chemistry. In this work, we report the isolation, structure elucidation, and bioactivity screening of cellulamides A and B (1 and 2), two novel linear peptides obtained from the culture of the macroalga-associated Cellulosimicrobium funkei CT-R177. The host of this microorganism, the Chlorophyta Codium tomentosum, was collected in the northern Portuguese coast and, in the scope of a bioprospecting study focused on its associated actinobacterial community, strain CT-R177 was isolated, taxonomically identified, and screened for the production of antimicrobial and anticancer compounds. Dereplication of a crude extract of this strain using LC-HRMS(/MS) analysis unveiled a putative novel natural product, cellulamide A (1), that was isolated following mass spectrometry-guided fractionation. An additional analog, cellulamide B (2) was obtained during the chromatographic process and chemically characterized. The chemical structures of the novel linear peptides, including their absolute configurations, were elucidated using a combination of HRMS, 1D/2D NMR spectroscopy, and Marfey’s analysis. Cellulamide A (1) was subjected to a set of bioactivity screenings, but no significant biological activity was observed. The cellulamides represent the first family of natural products reported from the Actinomycetota genus Cellulosimicrobium, showcasing not only the potential of less-explored taxa but also of host-associated marine strains for novel chemistry discovery.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cellulamides: A New Family of Marine-Sourced Linear Peptides from the Underexplored Cellulosimicrobium Genus

Girão,

Murillo-Alba,

Martín

et al. 2024

Marine Drugs

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“…Brevibacterium sp. MOSEL-ME10a has been previously characterized as B. luteolum [90], however with B. luteolum strains, it shares an ANIb as high as 92.6%, therefore, it is improbable that strain MOSEL-ME10a belongs to the species B. luteolum. From clade II-B, Brevibacterium sp.…”

Section: Average Nucleotide Identity Analysismentioning

confidence: 99%

Exploring the Biosynthetic Gene Clusters in Brevibacterium: A Comparative Genomic Analysis of Diversity and Distribution

Cumsille

Serna-Cardona

González

et al. 2023

Preprint

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Exploring Brevibacterium strains from various ecosystems may lead to the discovery of new antibiotic-producing strains. Brevibacterium sp. H-BE7, a strain isolated from marine sediments from Northern Patagonia, Chile, exhibited antimicrobial activity against Salmonella enterica and Listeria monocytogenes. Chemical dereplication identified bioactive compounds, such as 1-methoxyphenazine in the crude extracts of strain H-BE7, which could be responsible of the observed antibacterial activity. The genome of Brevibacterium sp. H-BE7 was sequenced and a phenazine-like biosynthetic gene clusters (BGCs) is not present within the genome. To study the biosynthetic potential of strain H-BE7 and Brevibacterium genus, the genome sequences of 98 Brevibacterium strains, including strain H-BE7, were selected for a genomic analysis. A phylogenomic cladogram was generated, which divided the Brevibacterium strains into four major clades. A total of 25 strains are potentially unique new species according to Average Nucleotide Identity (ANIb) values. These strains were isolated from various environments, emphasizing the importance of exploring diverse ecosystems to discover the full diversity of Brevibacterium. Pangenome analysis of Brevibacterium strains revealed that only 2.5% of gene clusters are included within the core genome, and most gene clusters occur either as singletons or as cloud genes present in less than ten strains. Brevibacterium strains from various phylogenomic clades exhibit diverse BGCs. Specific groups of BGCs show clade-specific distribution patterns, such as siderophore BGCs and carotenoid-related BGCs. A group of clade IV-A Brevibacterium strains possess a clade-specific Polyketide synthase (PKS) BGCs that connects with phenazine-related BGCs and could be related to the production of 1-methoxyphenazine in HBE-7’s crude extract. Within the PKS BGC, five genes, including the biosynthetic PKS gene, participate in the mevalonate pathway and exhibit similarities with the phenazine A BGC. However, additional core biosynthetic phenazine genes were exclusively discovered in nine Brevibacterium strains, primarily isolated from cheese. While strain H-BE7 lacks the core phenazine biosynthetic genes, it produces 1-methoxyphenazine, indicating the presence of an unknown biosynthetic pathway for this compound. This suggests the existence of alternative biosynthetic pathways or promiscuous enzymes within H-BE7's genome.

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“…are known producers of anticancer compounds. Related studies have shown that the Protobacterium luteum MOSEL-ME10a, found in the ocean, shows a significant inhibitory effect against HepG2 cells ( Tanveer et al, 2021 ). Actinomycete species extracted from marine sediments have also been described to produce metabolites with potent anticancer activity, such as ZHD-0501, a species that has been shown to inhibit the proliferation of human liver cancer and leukemia cells ( Aly et al, 2021 ).…”

Section: Therapeutic Effect Of Bioactive Substances On Liver Cancermentioning

confidence: 99%

The pathogenesis of liver cancer and the therapeutic potential of bioactive substances

Gao

Jiang²,

Wang³

et al. 2022

Front. Pharmacol.

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Liver cancer is the third most common cause of cancer-related deaths in the world and has become an urgent problem for global public health. Bioactive substances are widely used for the treatment of liver cancer due to their widespread availability and reduced side effects. This review summarizes the main pathogenic factors involved in the development of liver cancer, including metabolic fatty liver disease, viral infection, and alcoholic cirrhosis, and focuses on the mechanism of action of bioactive components such as polysaccharides, alkaloids, phenols, peptides, and active bacteria/fungi. In addition, we also summarize transformation methods, combined therapy and modification of bioactive substances to improve the treatment efficiency against liver cancer, highlighting new ideas in this field.

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Genome sequence analysis and bioactivity profiling of marine-derived actinobacteria, Brevibacterium luteolum, and Cellulosimicrobium funkei

Cited by 5 publications

References 50 publications

Cellulamides: A New Family of Marine-Sourced Linear Peptides from the Underexplored Cellulosimicrobium Genus

Cellulamides: A New Family of Marine-Sourced Linear Peptides from the Underexplored Cellulosimicrobium Genus

Exploring the Biosynthetic Gene Clusters in Brevibacterium: A Comparative Genomic Analysis of Diversity and Distribution

The pathogenesis of liver cancer and the therapeutic potential of bioactive substances

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