Genome sequence and description of the mosquitocidal and heavy metal tolerant strain Lysinibacillus sphaericus CBAM5

Peña-Montenegro, Tito David; Lozano, Lucía C.; Dussán, Jenny

doi:10.1186/1944-3277-10-2

Cited by 48 publications

(25 citation statements)

References 55 publications

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“…In addition, according to OT4b.31, CBAM5, and III(3)7 strain genomes, there were annotated genes involved in the denitrification processes [8][9][10] such as nitric oxide and nitrous oxide reduction to molecular nitrogen. In addition, according to OT4b.31, CBAM5, and III(3)7 strain genomes, there were annotated genes involved in the denitrification processes [8][9][10] such as nitric oxide and nitrous oxide reduction to molecular nitrogen.…”

Section: Sphaericus Nitrogen Fixer and Nitrifying Bacteriamentioning

confidence: 99%

Lysinibacillus sphaericus plant growth promoter bacteria and lead phytoremediation enhancer with Canavalia ensiformis

Martínez

Dussán

2017

Env Prog and Sustain Energy

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Lysinibacillus sphaericus is known for its great larvicidal activity against mosquito larvae and toxic metal resistance, but knowledge about its possible activity in soil is scarce and poor. Nevertheless, in the genome of some strains have been reported genes of nitrogen cycle. Seven L. sphaericus strains were tested as nitrogen fixing bacteria, nitrifying bacteria, and indoleacetic acid producer. Subsequently a consortium was formulated using the best strains (CBAM5, III(3)7, OT4b.31, and OT4b.49) to evaluate its effect in Canavalia ensiformis growth and lead phytoremediation. All L. sphaericus strains were able to fix nitrogen (0.61–1.47 mg/L of ammonium), nitrify (0.26–0.7 mg/L of nitrate), and produce IAA (3.3–5.5 µg/mL in L‐tryptophan media and 0.3–1.1 µg/mL in trypticase broth). They also promoted shoot length, root length, foliar area, and leaf number in C. ensiformis, enhanced lead bioaccumulation in the root reaching a removal of 40% of lead in greenhouse experiment, and increased nitrate content in soil twofold in contrast to the control in field experiment. Therefore, L. sphaericus could be a key organism in the formulation of biofertilizers, as it shows potential in the phytoremediation processes, in crop plant nutrition, and growth in low nutrient polluted soils, then is a plant growth promoter bacteria (PGPB). © 2017 American Institute of Chemical Engineers Environ Prog, 37: 276–282, 2018

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Section: Sphaericus Nitrogen Fixer and Nitrifying Bacteriamentioning

confidence: 99%

Lysinibacillus sphaericus plant growth promoter bacteria and lead phytoremediation enhancer with Canavalia ensiformis

Martínez

Dussán

2017

Env Prog and Sustain Energy

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“…sphaericus CBAM5 is responsible for the great efficiency as these living bacteria present active and passive mechanisms for gold removal, such as absorption and adsorption, respectively [14]. In the case of adsorption, it has previously been shown that this bacterium presents an S-Layer protein that assembles in the cell wall and acts as an ion trap [18]. Moreover, it is known that this protein possesses functional groups with affinity to metals like Au(III) and thus, complexation may occur after the exposure of L. sphaericus CBAM5 to that metal ion.…”

Section: Discussionmentioning

confidence: 99%

“…The strain L. sphaericus CBAM5 has shown a great potential to capture several metal ions like gold and mercury [15][16][17]. A study of its genomic sequence has shown that it possesses several coding regions for S-Layer proteins and it presents heavy metal efflux pumps that help the cell to resist high concentrations of metal ions [13,18].…”

Section: Introductionmentioning

confidence: 99%

Effective Gold Biosorption by Electrospun and Electrosprayed Bio-Composites with Immobilized Lysinibacillus sphaericus CBAM5

2020

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Electro-hydrodynamic processing, comprising electrospinning and electrospraying techniques, is a novel technology used in the production of nano- and sub-micro-scale materials with specific properties suitable for environmental remediation processes. Polycaprolactone (PCL) micro-fibrous mats and alginate microcapsules were produced using electrospinning and electrospraying techniques respectively, and Lysinibacillus sphaericus CBAM5, a bacterium capable of metal removal by adsorption and accumulation inside the cell, was immobilized in these matrices. The polymeric structure was able to protect and maintain cell viability and the bio-composite materials were used to capture gold from synthetic water samples. The micro-fibrous membranes with immobilized bacteria were able to remove 93% of the gold after 120 h of inclusion in the aqueous medium. Using a filtration system, an efficiency of 64% was obtained for the removal of the precious metal after 10 cycles of filtration (2 h of exposure to the gold solution). In contrast, the microencapsulated L. sphaericus CBAM5 captured 64% of the gold after 4 h of the assay. Thus, both micro-structured matrices were suitable for the immobilization and protection of L. sphaericus CBAM5 and they showed high efficiencies of gold biosorption. Hence, these bio-composite materials could be used to concentrate gold from industrial wastewaters.

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“…The recent sequencing of L. sphaericus Ot4b31 genome (Peña‐Montenegro & Dussán, ) and resequencing of L. sphaericus CBAM5 genome (Peña‐Montenegro et al., ) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis identified proteins that might participate in peripheral pathways for degradation of benzoate, amino benzoate, toluene, and other aromatic compounds, which might be an indication of the ability of this genera to biodegrade TPH.…”

Section: Introductionmentioning

confidence: 99%

Lysinibacillus sphaericus and Geobacillus sp Biodegradation of Petroleum Hydrocarbons and Biosurfactant Production

Manchola¹,

Dussán²

2014

Remediation Journal

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Petroleum oil is a major driver of worldwide economic activity, but it has also created contamination problems during the storage and refining process. Also, unconventional resources are natural resources, which require greater than industry‐standard levels of technology or investment to exploit. In the case of unconventional hydrocarbon resources, additional technology, energy, and capital have to be applied to extract the gas or oil. Bioremediation of petroleum spill is considered of great importance due to the contaminating effects on human health and the environment. For this reason, it is important to reduce total petroleum hydrocarbons (TPH) in contaminated soil. In addition, biosurfactant production is a desirable property of hydrocarbon‐degrading microorganisms. Seven strains belonging to Lysinibacillus sphaericus and Geobacillus sp were selected to evaluate their ability to biodegrade TPH in the presence of toxic metals, their potential to produce biosurfactants, and their ability to improve the biodegradation rate. The seven bacterial strains examined in this study were able to utilize crude petroleum‐oil hydrocarbons as the sole source of carbon and energy. In addition, their ability to degrade crude oil was not affected by the presence of toxic metals such as chromium and arsenic. At the same time, the strains were able to reduce toxic metals concentration through biosorption processes. Biosurfactant production was determined using the drop‐collapsed method for all strains, and they were characterized as both anionic and cationic biosurfactants. Biosurfactants showed an increase in biodegradation efficiency both in liquid minimal salt medium and landfarming treatments. The final results in field tests showed an efficiency of 93 percent reduction in crude oil concentration by the selected consortium compared to soil without consortium. The authors propose L. sphaericus and Geobacillus sp consortium as an optimum treatment for contaminated soils. In addition, production of biosurfactants could have an application in the extraction of crude oil from unconventional hydrocarbon resources. © 2014 Wiley Periodicals, Inc.

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Genome sequence and description of the mosquitocidal and heavy metal tolerant strain Lysinibacillus sphaericus CBAM5

Cited by 48 publications

References 55 publications

Lysinibacillus sphaericus plant growth promoter bacteria and lead phytoremediation enhancer with Canavalia ensiformis

Lysinibacillus sphaericus plant growth promoter bacteria and lead phytoremediation enhancer with Canavalia ensiformis

Effective Gold Biosorption by Electrospun and Electrosprayed Bio-Composites with Immobilized Lysinibacillus sphaericus CBAM5

Lysinibacillus sphaericus and Geobacillus sp Biodegradation of Petroleum Hydrocarbons and Biosurfactant Production

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