Genome Sequence of Salmonella enterica subsp.
            <i>enterica</i>
            Serovar Typhi Isolate PM016/13 from Untreated Well Water Associated with a Typhoid Outbreak in Pasir Mas, Kelantan, Malaysia

Harish, Salwani Muhamad; Sim, Kee-Shin; Najimudin, Nazalan; Ismail, Asma

doi:10.1128/genomea.01261-15

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…Typhi whole-genome sequences available in NCBI were used for data mining. They comprised CT18 (Genbank accession number AL513382) [27], Ty2 (Genbank accession number AE014613) [28], P-stx-12 (Genbank accession number CP003278) [29], Ty21a (Genbank accession number CP002099) [30], B/SF/13/03/195 (Genbank accession number CP012151) [31], and PM016/13 (Genbank accession number CP012091) [32]. In order to ascertain whether the genomic regions were conserved and specific to S .…”

Section: Methodsmentioning

confidence: 99%

Identification of Five NovelSalmonellaTyphi-Specific Genes as Markers for Diagnosis of Typhoid Fever Using Single-Gene Target PCR Assays

Goay

Chin

Tan

et al. 2016

BioMed Research International

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Salmonella Typhi (S. Typhi) causes typhoid fever which is a disease characterised by high mortality and morbidity worldwide. In order to curtail the transmission of this highly infectious disease, identification of new markers that can detect the pathogen is needed for development of sensitive and specific diagnostic tests. In this study, genomic comparison of S. Typhi with other enteric pathogens was performed, and 6 S. Typhi genes, that is, STY0201, STY0307, STY0322, STY0326, STY2020, and STY2021, were found to be specific in silico. Six PCR assays each targeting a unique gene were developed to test the specificity of these genes in vitro. The diagnostic sensitivities and specificities of each assay were determined using 39 S. Typhi, 62 non-Typhi Salmonella, and 10 non-Salmonella clinical isolates. The results showed that 5 of these genes, that is, STY0307, STY0322, STY0326, STY2020, and STY2021, demonstrated 100% sensitivity (39/39) and 100% specificity (0/72). The detection limit of the 5 PCR assays was 32 pg for STY0322, 6.4 pg for STY0326, STY2020, and STY2021, and 1.28 pg for STY0307. In conclusion, 5 PCR assays using STY0307, STY0322, STY0326, STY2020, and STY2021 were developed and found to be highly specific at single-gene target resolution for diagnosis of typhoid fever.

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