Genome sequence of the white-rot fungus Irpex lacteus F17, a type strain of lignin degrader fungus

Abstract

Irpex lacteus, a cosmopolitan white-rot fungus, degrades lignin and lignin-derived aromatic compounds. In this study, we report the high-quality draft genome sequence of I. lacteus F17, isolated from a decaying hardwood tree in the vicinity of Hefei, China. The genome is 44,362,654 bp, with a GC content of 49.64% and a total of 10,391 predicted protein-coding genes. In addition, a total of 18 snRNA, 842 tRNA, 15 rRNA operons and 11,710 repetitive sequences were also identified. The genomic data provides insights into the mechanisms of the efficient lignin decomposition of this strain.Electronic supplementary materialThe online version of this article (10.1186/s40793-017-0267-x) contains supplementary material, which is available to authorized users.

Deciphering lignocellulose deconstruction by the white rot fungus Irpex lacteus based on genomic and transcriptomic analyses

“…Previous to our study, there was only one brief report on the genome sequence of an I. lacteus strain F17 [ 21 ]. In this study, we found that the genome of I. lacteus CD2 is 43.16 Mb, similar to but slightly smaller than that of formerly sequenced I. lacteus F17 (44.36 Mb).…”

“…In this study, we found that the genome of I. lacteus CD2 is 43.16 Mb, similar to but slightly smaller than that of formerly sequenced I. lacteus F17 (44.36 Mb). Despite this similarity, however, I. lacteus CD2 encodes higher numbers of plant cell wall polysaccharide-degrading enzymes, with 182 GHs compared to 161 GHs in I. lacteus F17 [ 21 ]. In addition, I. lacteus CD2 does not have a laccase, but have 7 MnPs, 2 LiPs, and 4 DyPs, while I. lacteus F17 bears one laccase, one LiP, 13 MnPs but no DyP.…”

“…Therefore, it is not known at this time whether the difference in the numbers and amino acid sequences of encoding enzymes by the two strains would impact their efficiency in lignocellulose degradation. The 18S rRNA phylogenetic analysis suggested that I. lacteus F17 is closest to C. subvermispora , a selective lignin degrader [ 21 ]. Taxonomy based on 18S rRNA gene showed a limitation for certain order level [ 58 – 60 ], while the phylogenomic approach could improve phylogenetic accuracy.…”

“…238 617/93 [ 20 ]. Recently, the genome of an I. lacteus strain F17 was reported [ 21 ]. The secretome of I. lacteus strain Fr.…”

Comprehensive investigation of a dye-decolorizing peroxidase and a manganese peroxidase from Irpex lacteus F17, a lignin-degrading basidiomycete

“…The 14 PODs enzymes include one LiP and 13 putative MnPs. Phylogenetic analysis based on 18S rRNA sequences showed that I. lacteus F17 was closely related to Ceriporiopsis subvermispora and Phanerochaete chrysosporium (Yao et al 2017 ). Interestingly, the genome of C. subvermispora also encodes 13 MnPs, whereas it has no genes encoding DyPs, similar to P. chrysosporium (Fernández-Fueyo et al 2012 ; Floudas et al 2012 ).…”