Genome sequences and SNP analyses of Corynespora cassiicola from cotton and soybean in the southeastern United States reveal limited diversity

Abstract

Corynespora cassiicola attackes diverse agriculturally important plants, including soybean and cotton, in the US. It is a reemerge pathogen on cotton in southeastern US. Whole genome sequences of four cotton and one soybean isolate from Tennessee were used to develop single nucleotide polymorphism markers for cotton isolates. Cotton isolates had little diversity at the genome level and very little differentiation from the soybean isolate. Analysis of 75 isolates from cotton and soybean, using targeted-sequencing of 22 polymorphic SNP sites, revealed eight multi-locus genotypes and it appears a single clonal lineage predominates across the southeastern region. The cotton and soybean genome sequences were significantly different from the public reference genome derived from a rubber isolate and the utility of these novel resources will be discussed.

Compounds Released by the Biocontrol Yeast Hanseniaspora opuntiae Protect Plants Against Corynespora cassiicola and Botrytis cinerea

“…In the plants, this characterization includes, the analysis of the early events during the beneficial and pathogenic interactions (Zipfel and Oldroyd, 2017 ), the transcriptional regulation of plant defense responses (Birkenbihl et al, 2017 ) and the elicitor-mediated activation of plant immunity (Cheng et al, 2018 ). On the other hand, the molecular analysis of the pathogens Corynespora cassiicola and Botrytis cinerea also has also been improved with the identification of the genomic sequence and the transcriptomic characterization during the interaction with the plants (Windram et al, 2012 ; Shrestha et al, 2017 ; Van Kan et al, 2017 ). Now, with all this available information and with the HoFs-induced DEGs identified from our work, further studies are warranted, that might help us to understand the molecular defense mechanisms induced by HoFs.…”

The fungal pathogen Corynespora cassiicola: A review and insights for target spot management on cotton and Soya bean

“…Cotton and Soya bean C. cassiicola isolates from a single location in Tennessee revealed eight unique multilocus genotypes, in contrast to isolates from south-eastern United States that formed a single clone (Shrestha et al, 2017 (Lopez et al, 2018).…”

“…The wide host range of C. cassiicola and its cultivar preference were explained based on the pathogen diversity (Déon et al, 2014). Different genes of C. cassiicola have been amplified to construct phylogenetic trees to understand the evolution and variability of C. cassiicola isolates and to help build disease management strategies (Banguela-Castillo et al, 2020;Bentes et al, 2018;Déon et al, 2014;Dixon et al, 2009;Hieu et al, 2020;Nghia et al, 2008;Oktavia et al, 2017;Qi et al, ,2009Qi et al, , , 2011Shimomoto et al, 2011;Shrestha et al, 2017;Silva et al, 2003;Wu et al, 2019). Dixon et al, (2009) conducted the most prominent study on (Qi et al, 2009).…”

Unraveling the host-selective toxic interaction of cassiicolin with lipid membranes and its cytotoxicity

“…C. cassiicola predominantly infects both young and mature leaves, and CLF disease has now become widespread in Hevea brasiliensis plantations, causing severe damage to the natural rubber industry. These pathogens have also been found in many other crops [2][3][4] .…”