Genome sequencing and functional annotation of Bacillus sp. strain BS-Z15 isolated from cotton rhizosphere soil having antagonistic activity against Verticillium dahliae

Chen, Zhongyi; Abuduaini, Xieerwanimu; Mamat, Nuramina; Yang, Qilin; Wu, Mengjun; Lin, Xinrui; Wang, Ru; Lin, Ronggui; Zeng, Weijun; Ning, Huan-Chen; Zhao, Heping; Li, Jinyu; Zhao, Huixin

doi:10.1007/s00203-020-02149-7

Cited by 10 publications

(8 citation statements)

References 55 publications

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“…Moreover, recent studies suggest that they also play a role in transcription modulation and enhance plant resistance against phytopathogens [15,61]. Siderophore such as bacilibactin, and bacilysin suppress fungal growth by competing for nutrition [62,63]. Interestingly, all three antagonist bacteria possess fenABCDE gene clusters responsible for the fengycin biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Probiotic <em>Bacillus</em> Species: Promising Biological Control Agents for Managing Worrisome Wheat Blast Disease

Surovy¹,

Dutta²,

Mahmud³

et al. 2022

Preprint

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Plant diseases are among the major factors affecting plant productivity. Biological control of plant diseases is preferred over chemical control as it is environment-friendly, cost-effective, and sustainable. Among many microbes capable of providing biological control of plant diseases, probiotic Bacillus species are most promising as they can survive in adverse conditions, provide plants with a wide range of benefits including protection from phytopathogens. Wheat blast caused by Magnaporthe oryzae Triticum pathotype (MoT) has emerged as a potential threat to global wheat production. Due to unreliability of fungicides and limited cultivar resistance, we aimed to screen and identify potential antagonist bacteria collected from internal tissues of rice and wheat seeds to determine their in vitro and in vivo inhibitory effects against MoT. Dual culture and seedling assays were performed to evaluate the efficacy of probiotic bacteria. Out of 170 bacterial isolates, three bacteria (BTS-3, BTS-4, and BTLK6A) were screened as potential antagonists against MoT in vitro. Artificial inoculation at the seedling stage showed that the isolates BTS-4, BTS–3, and BTLK6A reduced 89, 88, and 85% of wheat blast disease severity, respectively, compared to mock-inoculated control. The bacterial isolates were identified as Bacillus subtilis (BTS-3) and B. velezensis (BTS-4 and BTLK6A) through genome phylogeny. The whole genome sequence of these three bacterial strains decoded a number of orthologs to intrinsic genes of antimicrobial peptides, antioxidant defense enzymes, cell wall degrading enzymes, compounds involved in the induction of systemic resistance (ISR) in host plants, and volatile compounds to make them promising biologicals to control MoT in wheat. Combined data of in vitro and in vivo along with genome analysis suggest that Bacillus spp. suppress the destructive wheat blast disease likely through antibiosis and ISR in the host plants. Further field evaluation and characterization of antimicrobial compounds are needed for a better understanding of the mode of action and practical recommendation of these bacteria for wheat blast control in the farmers’ fields.

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Section: Discussionmentioning

confidence: 99%

Probiotic <em>Bacillus</em> Species: Promising Biological Control Agents for Managing Worrisome Wheat Blast Disease

Surovy¹,

Dutta²,

Mahmud³

et al. 2022

Preprint

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“…GZDF3 were active against C. albicans [ 29 ], and it is known that iron plays a crucial role in C. albicans growth [ 30 ]. According to the genomic analysis, B. cereus ILBB55 showed a cluster 46% similar to another known cluster that codes for bacillibactin, suggesting that it could be a variant of that compound [ 31 ]. In a recent report, isolates which showed antimicrobial activity were screened for the presence of NRPS [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Bioprospecting the Antibiofilm and Antimicrobial Activity of Soil and Insect Gut Bacteria

et al. 2022

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Antimicrobial resistance is a growing concern in public health and current research shows an important role for bacterial biofilms in recurrent or chronic infections. New strategies, therefore, are necessary to overcome antimicrobial resistance, through the development of new therapies that could alter or inhibit biofilm formation. In this sense, antibiofilm natural products are very promising. In this work, a bioprospection of antimicrobial and antibiofilm extracts from Uruguayan soil bacteria and insect gut bacteria was carried out. Extracts from extracellular broths were tested for their ability to inhibit planktonic cell growth and biofilm formation. Genomic analysis of Bacillus cereus ILBB55 was carried out. All extracts were able to inhibit the growth of, at least, one microorganism and several extracts showed MICs lower than 500 µg mL−1 against microorganisms of clinical relevance (Staphylococcus aureus, Pseudomonas aeruginosa, and Enterobacter cloacae). Among the extracts evaluated for biofilm inhibition only ILBB55, from B. cereus, was able to inhibit, S. aureus (99%) and P. aeruginosa (62%) biofilms. Genomic analysis of this strain showed gene clusters similar to other clusters that code for known antimicrobial compounds. Our study revealed that extracts from soil bacteria and insect gut bacteria, especially from B. cereus ILBB55, could be potential candidates for drug discovery to treat infectious diseases and inhibit S. aureus and P. aeruginosa biofilms.

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“…BS-Z15 genome was sequenced and deposited to NCBI database under the accession number QOCJ00000000, BioSample SAMN09582630 and BioProject PRJNA479656 [ 32 ]. Then, four ORFs for mycosubtilin synthase genes, fenF , mycA , mycB , and mycC from Bacillus subtilis ATCC6633 [ 29 ] were used to blast BS-Z15 genome to characterize the homologous genes.…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of a mycosubtilin homologue antagonizing Verticillium dahliae produced by Bacillus subtilis strain Z15

Lin

Zhang

et al. 2022

PLoS ONE

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Bacillus subtilis strain Z15 (BS-Z15) was isolated from the cotton field of Xinjiang, China, and characterized as an effective biocontrol agent antagonizing plant pathogen Verticillium dahliae 991 (VD-991). However, the chemical substance produced by BS-Z15 for resistance to VD-991 remains elusive. Here, a serial purification methods including HCl precipitation, organic solvent extraction, and separation by semi-preparative High-Performance Liquid Chromatography were performed to obtain a single compound about 3.5 mg/L from the fermentation broth of BS-Z15, which has an antifungal activity against VD-991. Moreover, Fourier Transform Infrared spectrum, Nuclear Magnetic Resonance Spectroscopy, and Tandem Mass Spectrometry analyses were carried out to finally confirm that the active compound from BS-Z15 is a mycosubtilin homologue with C17 fatty acid chain. Genomic sequence prediction and PCR verification further showed that the BS-Z15 genome contains the whole mycosubtilin operon comprising four ORFs: fenF, mycA, mycB, and mycC, and the expression levels of mycA-N, mycB-Y and mycC-N reached a peak at 32-h fermentation. Although mycosubtilin homologue at 1 μg/mL promoted the germination of cotton seed, that with high concentration at 10 μg/mL had no significant effect on seed germination, plant height and dry weight. Furthermore, mycosubtilin homologue sprayed at 10 μg/mL on two-week-old cotton leaves promotes the expression of pathogen-associated genes and gossypol accumulation, and greatly decreases VD-991 infection in cotton with disease index statistics. This study provides an efficient purification strategy for mycosubtilin homologue from BS-Z15, which can potentially be used as a biocontrol agent for controlling verticillium wilt in cotton.

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Genome sequencing and functional annotation of Bacillus sp. strain BS-Z15 isolated from cotton rhizosphere soil having antagonistic activity against Verticillium dahliae

Cited by 10 publications

References 55 publications

Probiotic <em>Bacillus</em> Species: Promising Biological Control Agents for Managing Worrisome Wheat Blast Disease

Probiotic <em>Bacillus</em> Species: Promising Biological Control Agents for Managing Worrisome Wheat Blast Disease

Bioprospecting the Antibiofilm and Antimicrobial Activity of Soil and Insect Gut Bacteria

Isolation and characterization of a mycosubtilin homologue antagonizing Verticillium dahliae produced by Bacillus subtilis strain Z15

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