Genome sequencing as a new window into the microbial community of membrane bioreactors – A critical review

Permeate microbiome reflects the biofilm microbial community in a gravity-driven woven-fiber microfiltration (WFMF) system for wastewater treatment

“…This result is consistent with other studies that have found Pseudomonadota as one of the dominant taxa in activated sludge and MBR biofilms. 16,18–20,43–48 Pseudomonadota play an essential role in nutrient removal, as they include functional groups such as ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB), and phosphorus-accumulating organisms (PAO). 46,49…”

“…This result is consistent with other studies that have found Pseudomonadota as one of the dominant taxa in activated sludge and MBR biofilms. 16,[18][19][20][43][44][45][46][47][48] Pseudomonadota play an essential role in nutrient removal, as they include functional groups such as ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB), and phosphorusaccumulating organisms (PAO). 46,49 Three phyla with low relative abundance in the influent showed a considerably higher relative abundance in the biofilm and permeate: Chloroflexota, Actinomycetota, and Planctomycetota.…”

“…16,[18][19][20][43][44][45][46][47][48] Pseudomonadota play an essential role in nutrient removal, as they include functional groups such as ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB), and phosphorusaccumulating organisms (PAO). 46,49 Three phyla with low relative abundance in the influent showed a considerably higher relative abundance in the biofilm and permeate: Chloroflexota, Actinomycetota, and Planctomycetota. Chloroflexota may include filamentous bacteria and may be favored by higher MLSS concentrations in various membrane submerged bioreactors (i.e., MBRs).…”

“…Chloroflexota may include filamentous bacteria and may be favored by higher MLSS concentrations in various membrane submerged bioreactors (i.e., MBRs). 44 It has been suggested that Chloroflexota also may play a role in organic carbon removal, 46,50 and possibly in denitrification, 51 as well as the development of microbial flocs. 50,52 Like Chloroflexota, Actinomycetota is composed of filamentous bacteria.…”

“…In addition, given the observed role of the biofilm in shaping the permeate microbial community and the documented effect of operational settings on the microbial composition of the membrane biofilm, the possibility of controlling the microbial structure and processes by adjusting the operational settings of a filtration system has been raised. 36,46 Nevertheless, much remains unknown about membrane colonization, maturation and fouling and the ecological roles of the different taxa in the microbial communities. The effects of various operational settings on the microbial community have not been thoroughly studied considering the technical limitations that would make the analysis difficult in the field.…”