Genome sequencing of Enterococcus faecium NT04, an oral microbiota revealed the production of enterocin A/B active against oral pathogens

Tarek, Nashwa; Azmy, Ahmed F.; Khairalla, Ahmed S.; Abdel-Fattah, Maha M.; Jefri, Ohoud A.; Shaban, Mohamed; Elsayed, Abdelrahman H.; El-Gendy, Ahmed O.

doi:10.1016/j.heliyon.2023.e16253

Cited by 3 publications

(1 citation statement)

References 53 publications

(57 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Staphylococcus is commonly isolated as a contaminant in nosocomial and environmental samples [35] [36] [37]. However, its biofilms are a contribution to antimicrobial resistance due to the acquisition of virulence genes and their existence in pathogenicity islands [38] [39] [40].…”

Section: Identification Of Biofilm-producing Isolatesmentioning

confidence: 99%

Antimicrobial Activities of Extracts of <i>Macrosphyra longistyla</i> against Gram-Positive Oral Biofilm-Formers from School Children in Southwestern Nigeria and Toxicity Studies Using Brine Shrimps

Nwankwo,

Folarin,

Osho

2024

AiM

View full text Add to dashboard Cite

The world will benefit from more effective antimicrobial agents against oral conditions arising from the actions of biofilm forming bacteria. Also, information is lacking on the oral biofilm-forming bacterial diversity in Southwestern Nigeria. In this study, we isolate and characterize oral biofilm producing bacteria in the oral cavities of schoolchildren in Southwestern Nigeria.We also investigate the antimicrobial properties of Macrosphyra longistyla extracts against the biofilm-formers and the toxicity of potent extracts. Samples were obtained from 109 schoolchildren aged 4 -14 years from Lagos, Oyo and Osun States. Agar well diffusion technique was used in the antimicrobial susceptibility testing. Toxicity testing was done using brine shrimps (Artemia salina). Biofilm-formers in this study are Klebsiella sp., Streptococcus sp., Staphylococcus sp., and Micrococcus sp. Ethanol leaf extracts had the highest activity against all biofilm-producing bacteria. Ethanol stem bark extract, which elicited activity against Klebsiella only, was found to be less toxic than the ethanol leaf extract. Staphylococcus showed >10 mm susceptibility to the ethanol and aqueous extracts of Macrosphyra longistyla. Streptococcus and Micrococcus were susceptible to the antimicrobial actions of the ethanolic leaf extracts. Although the ethanol extracts of the leaves had lower minimum inhibitory concentrations than the ethanol extracts of the stem bark, toxicity studies showed ethanol extracts of the stem-bark to be more toxic than the ethanol extracts of the leaves. In conclusion, ethanolic extracts of Macrosphyra longistyla show potential as sources of antimicrobials against gram-positive, oral biofilm-forming bacteria.

show abstract

Section: Identification Of Biofilm-producing Isolatesmentioning

confidence: 99%

Antimicrobial Activities of Extracts of <i>Macrosphyra longistyla</i> against Gram-Positive Oral Biofilm-Formers from School Children in Southwestern Nigeria and Toxicity Studies Using Brine Shrimps

Nwankwo,

Folarin,

Osho

2024

AiM

View full text Add to dashboard Cite

show abstract

Genomic Insights Into Enterococcus mundtii 203: A Promising Probiotic Candidate Isolated From Camel Feces

Ahadaf,

Azzouz,

Galiou

et al. 2024

Probiotics & Antimicro. Prot.

View full text Add to dashboard Cite

Probiotic and Postbiotic Potentials of Enterococcus faecalis EF-2001: A Safety Assessment

Han,

Shin,

Lee

et al. 2024

Pharmaceuticals

View full text Add to dashboard Cite

Background: Probiotics, which are live microorganisms that, when given in sufficient quantities, promote the host’s health, have drawn a lot of interest for their ability to enhance gut health. Enterococcus faecalis, a member of the human gut microbiota, has shown promise as a probiotic candidate due to its functional attributes. However, safety concerns associated with certain strains warrant comprehensive evaluation before therapeutic application. Materials and Methods: In this study, E. faecalis EF-2001, originally isolated from fecal samples of a healthy human infant, was subjected to a multi-faceted assessment for its safety and probiotic potential. In silico analysis, CAZyme, biosynthetic, and stress-responsive proteins were identified. Results: The genome lacked biogenic amine genes but contained some essential amino acid and vitamin synthetic genes, and carbohydrate-related enzymes essential for probiotic properties. The negligible difference of 0.03% between the 1st and 25th generations indicates that the genetic information of the E. faecalis EF-2001 genome remained stable. The live E. faecalis EF-2001 (E. faecalis EF-2001L) demonstrated low or no virulence potential, minimal D-Lactate production, and susceptibility to most antibiotics except some aminoglycosides. No bile salt deconjugation or biogenic amine production was observed in an in vitro assay. Hemolytic activity assessment showed a β-hemolytic pattern, indicating no red blood cell lysis. Furthermore, the EF-2001L did not produce gelatinase and tolerated simulated gastric and intestinal fluids in an in vitro study. Similarly, heat-killed E. faecalis EF-2001 (E. faecalis EF-2001HK) exhibits tolerance in both acid and base conditions in vitro. Further, no cytotoxicity of postbiotic EF-2001HK was observed in human colorectal adenocarcinoma HT-29 cells. Conclusions: These potential properties suggest that probiotic and postbiotic E. faecalis EF-2001 could be considered safe and retain metabolic activity suitable for human consumption.

show abstract

Genome sequencing of Enterococcus faecium NT04, an oral microbiota revealed the production of enterocin A/B active against oral pathogens

Cited by 3 publications

References 53 publications

Antimicrobial Activities of Extracts of <i>Macrosphyra longistyla</i> against Gram-Positive Oral Biofilm-Formers from School Children in Southwestern Nigeria and Toxicity Studies Using Brine Shrimps

Antimicrobial Activities of Extracts of <i>Macrosphyra longistyla</i> against Gram-Positive Oral Biofilm-Formers from School Children in Southwestern Nigeria and Toxicity Studies Using Brine Shrimps

Genomic Insights Into Enterococcus mundtii 203: A Promising Probiotic Candidate Isolated From Camel Feces

Probiotic and Postbiotic Potentials of Enterococcus faecalis EF-2001: A Safety Assessment

Contact Info

Product

Resources

About

Genome sequencing of Enterococcus faecium NT04, an oral microbiota revealed the production of enterocin A/B active against oral pathogens

Cited by 3 publications

References 53 publications

Antimicrobial Activities of Extracts of &lt;i&gt;Macrosphyra longistyla&lt;/i&gt; against Gram-Positive Oral Biofilm-Formers from School Children in Southwestern Nigeria and Toxicity Studies Using Brine Shrimps

Antimicrobial Activities of Extracts of &lt;i&gt;Macrosphyra longistyla&lt;/i&gt; against Gram-Positive Oral Biofilm-Formers from School Children in Southwestern Nigeria and Toxicity Studies Using Brine Shrimps

Genomic Insights Into Enterococcus mundtii 203: A Promising Probiotic Candidate Isolated From Camel Feces

Probiotic and Postbiotic Potentials of Enterococcus faecalis EF-2001: A Safety Assessment

Contact Info

Product

Resources

About

Antimicrobial Activities of Extracts of <i>Macrosphyra longistyla</i> against Gram-Positive Oral Biofilm-Formers from School Children in Southwestern Nigeria and Toxicity Studies Using Brine Shrimps

Antimicrobial Activities of Extracts of <i>Macrosphyra longistyla</i> against Gram-Positive Oral Biofilm-Formers from School Children in Southwestern Nigeria and Toxicity Studies Using Brine Shrimps