Genome Size and Pollen Viability as Taxonomic Criteria: Application to the Genus Hosta

Zonneveld, B. J. M.; Iren, F. van

doi:10.1055/s-2001-12900

Cited by 55 publications

(81 citation statements)

References 29 publications

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“…Where many species in a genus have the same chromosome numbers, differences in nuclear DNA content are often present and have proven to be very effective in delimiting infrageneric divisions in a number of taxa (Ohri 1998). Genome size has been demonstrated to often vary a factor 1.5-3 between taxa with identical chromosome numbers (Ohri 1998;Yokoya et al 2000;Zonneveld 2001;Zonneveld and van Iren 2001;Ellul et al 2002;Zonneveld et al 2003a, b;Hirsch et al 2004: Albach andGreilhuber 2004;Zonneveld and Duncan 2006). Moreover, Greilhuber (1998Greilhuber ( , 2005 has clearly shown that intraspecific variation of genome size is much less than assumed.…”

Section: Introductionmentioning

confidence: 99%

The systematic value of nuclear DNA content for all species of Narcissus L. (Amaryllidaceae)

Zonneveld

2008

Plant Syst Evol

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The taxonomy of all species of Narcissus (Amaryllidaceae), an important horticultural crop, has not been investigated recently. As a new approach, genome size was determined by flow cytometry with propidium iodide from 375 accessions. The somatic nuclear DNA contents (2C) were shown to range from 14 to 38 pg for the diploids. Narcissus assoanus and N. gaditanus are, based on their nuclear DNA content, removed from section Apodanthi and placed in a new section Juncifolii. The different ploidy levels and species involved were entangled for N. ''fernandesii'' s.l. and a new allotetraploid form is named here. Section Pseudonarcissus was much more heterogeneous in nuclear DNA content than expected. Sixty-five accessions of N. pseudonarcissus possessed, with 23.7 pg, similar amounts of DNA. However, several species from this section were clearly distinctive in nuclear DNA content. It runs from the diploid N. primigenius with 21.7 pg to the also diploid N. nevadensis with 38.2 pg. Also N. abscissus and N. moleroi are with about 26 pg clearly different from N. pseudonarcissus. For the first time, in 11 accessions, hexaploidy was found in N. pseudonarcissus ssp. bicolor. A new section Nevadensis with 30-39 pg of nuclear DNA was split off from the section Pseudonarcissus with now 21-27 pg. A nonoploid N. dubius with 96.3 pg has by far the highest amount of nuclear DNA and can be calculated to have the highest ploidy ever reported in Narcisssus. The total number of Narcissus species was determined as 36, nine more than in Flora Europaea and they were divided up in two subgenera and 11 sections. Flow cytometry is shown to produce easily obtainable and original systematic data that lead to new insights. Genome size or C-value turns out to be one of the most salient features to define the status of the species in the genus Narcissus.

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Section: Introductionmentioning

confidence: 99%

The systematic value of nuclear DNA content for all species of Narcissus L. (Amaryllidaceae)

Zonneveld

2008

Plant Syst Evol

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“…(Zonneveld & Van Iren, 2001), Galanthus L. (Zonneveld & al., 2003a), Agapanthus L'Hér. (Zonneveld & Duncan, 2003b), Nerine Herb (Zonneveld & Duncan, 2006), Narcissus L. (Zonneveld, 2008) and Tulipa L. has shown that nuclear DNA content of species and cultivars can contribute to the taxonomy of species and the origin of hybrids.…”

Section: Introductionmentioning

confidence: 99%

The involvement of Narcissus hispanicus Gouan in the origin of Narcissus bujei and of cultivated trumpet daffodils (Amaryllidaceae)

Zonneveld¹

2010

Anal. Jard. Bot. Madr.

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Zonneveld, B.J.M. 2010. The involvement of Narcissus hispanicus Gouan in the origin of Narcissus bujei and of cultivated trumpet daffodils (Amaryllidaceae). Anales Jard. Bot. Madrid 67(1): 29-39.To investigate the origin of cultivated trumpet daffodils the genome size (2C-value) of more than 100 old and new cultivars were measured. A large number of concolored yellow tetraploid cultivars with large coronas seem to be derived from a doubling of chromosomes of a hybrid of the two species N. hispanicus Gouan and N. pseudonarcissus L. This can also still be recognized by the presence of a black spot at the top of the anthers in about 15 % of the tetraploid cultivars. Assuming N. pseudonarcissus as one of the parents of the allotetraploid cultivars, species of trumpet daffodils of section Pseudonarcissi investigated earlier were compared. Doubling of the nuclear DNA content of 23. Para investigar el origen de los narcisos trompeta cultivados se midió el tamaño del genoma (valor 2C) de más de 100 cultivares viejos y nuevos. Un gran número de cultivares tetraploides amarillos de color uniforme y con grandes coronas parecían derivarse de una duplicación de cromosomas de un híbrido de las dos especies N. hispanicus Gouan y N. pseudonarcissus L. Esto también se observa por la presencia de una mancha negra en la parte superior de las anteras en alrededor del 15 % de los cultivares tetraploides. Tomando a N. pseudonarcissus por uno de los parentales de los cultivares alotetraploides, se compararon especies de narcisos trompeta de la sección Pseudonarcissi que ya hubiesen sido investigados anteriormente. La duplicación del contenido de ADN nuclear de 23,8 pg de N. pseudonarcissus queda por debajo de los valores hallados en los narcisos trompeta. Se evaluaron otras especies de narciso trompeta de la sección Pseudonarcissi con una cantidad más alta de ADN nuclear complementaria, tales como N. hispanicus, con 25.8 pg, N. poeticus L., con 26 pg y el hexaploide N. pseudonarcissus ssp. bicolor (L.) Baker, con 67.7 pg (Zonneveld, 2008), entre otras. N. hispanicus, con 25.8 pg, se diferencia claramente de N. pseudonarcissus, con 23.8 pg, y también se acepta aquí como especie. El contenido de ADN nuclear de N. bujei (Fern. Casas) Fern. Casas, con 30 pg, se adecua al hibridismo (antiguo) para N. bujei entre N. longispathus Pugsley (36 pg) y N. hispanicus Gouan (25,8 pg). Los tetraploides con tépalos blancos y coronas amarillas pueden obtenerse con el cruzamiento entre N. pseudonarcissus diploide y el hexaploide y bicolor N. pseudonarcissus ssp. bicolor (L.) Baker.Palabras clave: Narcissus hispanicus, origen de los narcisos trompeta, origen de N. bujei.

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“…The chopping was done with a new razor blade in a Petri dish in 0.25 ml nucleiisolation buffer to which 0.25 mg RNase/ml was added (Zonneveld and Van Iren 2001). After adding 1.75 ml propidium iodide solution (50 mg PI/l in isolation buffer), the suspension with nuclei was filtered through a 30-lm nylon filter.…”

Section: Flow Cytometric Measurement Of Dna 2c Valuementioning

confidence: 99%

“…Primitive angiosperms are now supposed to have had small genomes; increases up to a factor of 1,000 have occurred independently in various modern taxa (Leitch et al 1998). Flow cytometry was successfully used to measure the 2C-value for the genera Hosta Tratt., Helleborus L., Clivia Lindl., Nerine Herb., Agapanthus L'Hér., Galanthus L., Narcissus L., Gasteria Duval., Tulipa L., etc., by Zonneveld ( , 2008Zonneveld ( , 2009), Zonneveld and Van Iren (2001), Duncan (2003, 2006), Zonneveld and Van Jaarsveld (2005) and . In this paper it is shown that genome size alone is not sufficient to discriminate all species of Eucomis.…”

Section: Introductionmentioning

confidence: 99%

Genome sizes of Eucomis L’Hér. (Hyacinthaceae) and a description of the new species Eucomis grimshawii G.D.Duncan & Zonneveld

Zonneveld

Duncan

2009

Plant Syst Evol

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Nuclear genome size, as measured by flow cytometry with propidium iodide, was used to investigate the relationships within the genus Eucomis L'Hér. (Hyacinthaceae). Most species of Eucomis have the same basic chromosome number, x = 15. However, the somatic DNA 2C-value (2C) is shown to range from 21 to 31 pg for the diploids. The largest genome contains roughly 10 10 more base pairs than the smallest. Genome sizes are evaluated here in combination with available morphological and geographical data. Therefore, the taxonomy proposed here is not based on genome size alone. The genus Eucomis, as here determined, has 12 species. These can be divided into two groups: mainly dwarf diploid species and large-sized, tetraploid species. A small diploid plant, Eucomis (autumnalis subsp.) amaryllidifolia, is restored to species status, as a diploid subspecies seems incongruent with an allotetraploid Eucomis autumnalis. Moreover, as a diploid it is separated reproductively from the allotetraploid E. autumnalis. A new diploid species that has the lowest C value, E. grimshawii, is described here. On the basis of DNA content and other morphological characters, possible parents are suggested for all tetraploid species. Nuclear DNA content as measured by using flow cytometry may conveniently be used to produce systematic data. It is applicable even in dormant bulbs or sterile plants for the monitoring of the trade in bulbous species.

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Genome Size and Pollen Viability as Taxonomic Criteria: Application to the Genus Hosta

Cited by 55 publications

References 29 publications

The systematic value of nuclear DNA content for all species of Narcissus L. (Amaryllidaceae)

The systematic value of nuclear DNA content for all species of Narcissus L. (Amaryllidaceae)

The involvement of <i>Narcissus hispanicus</i> Gouan in the origin of <i>Narcissus bujei</i> and of cultivated trumpet daffodils (Amaryllidaceae)

Genome sizes of Eucomis L’Hér. (Hyacinthaceae) and a description of the new species Eucomis grimshawii G.D.Duncan & Zonneveld

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