Genome size variation and species relationships in the genus Hydrangea

Cerbah, M.; Mortreau, Eric; Brown, Spencer; Siljak-Yakovlev, S.; Bertrand, Hélène; Lambert, Claudie

doi:10.1007/s001220000529

Cited by 61 publications

(77 citation statements)

References 26 publications

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“…They can be attributed to chromosome pairing problems due to their lack of homology and are the expression of the typical behaviour of non-paired or univalent chromosomes. In addition to the different number of chromosomes (Cerbah et al 2001), Mortreau et al (2010) have shown significant differences in the chromosome organization between subsp. aspera Kawakami group and sargentiana, especially at the caryotypic level and that of the organization of heterochromatins and ribosomic sequences, confirming discrepancies between chromosomes and suspected pairing problems in our case.…”

Section: Discussionmentioning

confidence: 97%

“…The quantity of nuclear DNA and the ploidy level of the parents were previously assessed by Cerbah et al (2001) and Mortreau et al (2010) and are, respectively, equal to 4.67 pg/2C and 2n = 2x = 36 for Plant Breeding, 133, 536-541 (2014) …”

Section: Determination Of Ploidy Level Of Hybrids By Chromosome Countmentioning

confidence: 99%

“…McClint.) and has already been characterized both genetically and morphologically.In-depth genetic characterization was based on cytogenetic observations such as measurement of the quantity of nuclear DNA and karyotyping (Cerbah et al 2001, Mortreau et al 2010, as well as intersimple sequence repeats (ISSR) molecular markers (Mortreau et al 2003, Crespel et al 2012. To further understand genetic relationships within the H. aspera species complex, hybridizations were carried out, notably between the subsp.…”

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confidence: 99%

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Pollen viability and meiotic behaviour in intraspecific hybrids of Hydrangea aspera subsp. aspera Kawakami group x subsp. sargentiana

Crespel

Morel

2014

Plant Breeding

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Pollen viability and male meiosis in intraspecific hybrids of Hydrangea aspera subsp. aspera Kawakami group (2n = 2x = 36) and subsp. sargentiana (2n = 2x = 34) were investigated. Although it had been assumed that they were sterile, pollen viability was observed; it varied from 2.5% to 12.1%. The production of gametes with different chromosome numbers was implied by the analysis of the dispersion of the diameter distribution of pollen grains. Analysis of male meiosis made it possible to identify the origins with two major categories of meiotic aberrations: abnormal chromosome distribution (early chromosome migration at metaphase, lagging chromosomes at anaphase, micronuclei at telophase), leading to the formation of unbalanced tetrads and/or ones with supernumerary microspores; and abnormal spindle orientation in metaphase II (tripolar, fused and parallel spindles), leading to the formation of dyads or triads. The mode of 2n pollen formation is of the First Division Restitution type. The high level of parental heterozygosity that is normally associated with them should facilitate the transfer of a polygenic trait in breeding programme.Key words: pollen diameter -abnormal chromosome distribution -disoriented metaphase II spindles -2n pollen grain -first division restitutionThe genus, Hydrangea, comprises 23 species (McClintock 1957), the majority originating in Asia. Generally, most of these species are poorly known at both biological and horticultural levels. The most horticulturally important species are H. macrophylla (Thunb.) Ser. and H. paniculata Sieb. that have been used as ornamental garden plants for a long time. However, other species such as H. aspera D. Don are also of horticultural interest, because of the colour of their inflorescences and plant shapes. This species contains four subspecies (H. aspera D. Don subsp. aspera Villosa and Kawakami groups, subsp. strigosa (Redher) E.M. McClint., subsp. robusta (Hook. f. & Thomson) E.M. McClint. and subsp. sargentiana (Redher) E.M. McClint.) and has already been characterized both genetically and morphologically.In-depth genetic characterization was based on cytogenetic observations such as measurement of the quantity of nuclear DNA and karyotyping (Cerbah et al. 2001, Mortreau et al. 2010, as well as intersimple sequence repeats (ISSR) molecular markers (Mortreau et al. 2003, Crespel et al. 2012. To further understand genetic relationships within the H. aspera species complex, hybridizations were carried out, notably between the subsp. aspera Kawakami group and subsp. sargentiana, the most distant at the cytogenetic level (Mortreau and Lambert, unpublished data). A viable progeny set of ten individuals was obtained.Based on descriptors recommended by the Union for the Protection of New Varieties of Plants (UPOV) for examining distinctiveness, homogeneity and stability (UPOV, 1991), a first qualitative morphological characterization of H. aspera was carried out by Bertrand et al. (2007). This characterization mainly concerned foliage and inflorescen...

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Section: Discussionmentioning

confidence: 97%

Section: Determination Of Ploidy Level Of Hybrids By Chromosome Countmentioning

confidence: 99%

mentioning

confidence: 99%

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Pollen viability and meiotic behaviour in intraspecific hybrids of Hydrangea aspera subsp. aspera Kawakami group x subsp. sargentiana

Crespel

Morel

2014

Plant Breeding

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“…The choice of subsection Asperae, in this study, arose from three reasons: the publication of these classifications which raise ambiguities concerning H. aspera, the unexpected differences in genome size revealed among seven accessions showing heterogeneity in this species (Cerbah et al 2001) and the presence of two particular chromosomes numbers (2n=30 for H. involucrata and 2n=34 for H. aspera) which are different from other species of Hydrangea genus (2n=36).…”

Section: Introductionmentioning

confidence: 95%

Cytogenetic characterization of Hydrangea involucrata Sieb. and H. aspera D. Don complex (Hydrangeaceae): genetic, evolutional, and taxonomic implications

Mortreau¹,

Siljak-Yakovlev²,

Cerbah³

et al. 2009

Tree Genetics & Genomes

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The subsection Asperae of genus Hydrangea L. (Hydrangeaceae) has been investigated for three reasons: several ambiguous classifications concerning Hydrangea aspera have been published, unexpected differences in genome size among seven accessions have been reported Cerbah et al. (Theor Appl Genet 103:45-51, 2001), and two atypical chromosome numbers (2n=30 for Hydrangea involucrata and 2n=34 for H. aspera) have been found when all other species of the genus present 2n=36. Therefore, these two species and four subspecies of Hydrangea in all 29 accessions were analyzed for their genome size, chromosome number, and karyotype features. This investigation includes flow cytometric measurements of nuclear DNA content and bases composition (GC%), fluorochrome banding for detection of GC-and AT-rich DNA regions, and fluorescent in situ hybridisation (FISH) for chromosome mapping of 5 S and 18 S-5.8 S-26 S rDNA genes. In the H. aspera complex, the genome size ranged from 2.98 (subsp. sargentiana) to 4.67 pg/2C (subsp. aspera), an exceptional intraspecific variation of 1.57-fold. The mean base composition was 40.5% GC. Our report establishes the first karyotype for the species H. involucrata, and for the subspecies of H. aspera which indeed present different formulae, offering an element of discrimination. FISH and fluorochrome banding revealed the important differentiation between these two species (H. involucrata and H. aspera) and among four subspecies of the H. aspera complex. Our results are in agreement with the Chinese classification that places the groups Kawakami and Villosa as two different species: Hydrangea villosa Rehder and Hydrangea kawakami Hayata. This knowledge can contribute to effective germplasm management and horticultural use.

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“…macrophylla is diploid (2n = 36; Cerbah et al, 2001), with a 2C value of 4.5 pg and an estimated genome size of 2.3 Gb (Zonneveld et al, 2005). Linkage maps are available for most ornamentals, including roses, chrysanthemums, carnations, and petunias (Rout and Mohapatra, 2006;Yagi, 2015).…”

Section: Introductionmentioning

confidence: 99%

Development of DNA Markers Linked to Double-Flower and Hortensia Traits in <i>Hydrangea macrophylla</i> (Thunb.) Ser.

et al. 2018

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Double flower and hortensia (mophead) hydrangea (Hydrangea macrophylla (Thunb.) Ser.) traits are recessively inherited. Cross breeding of these traits in hydrangea is difficult because it takes about two years from crossing to flowering. In this study, we aimed to obtain DNA linkage markers that would allow accelerated selection of these traits. We used next-generation sequencing to comprehensively collect DNA sequences from the 'Kirakiraboshi' with a double flower and lacecap inflorescence and the 'Frau Yoshimi' with a single flower and hortensia inflorescence, and designed simple sequence repeat (SSR) primer pairs for map construction. We screened 768 SSR primer pairs in 93 F 2 progeny derived from 'Kirakiraboshi' and 'Frau Yoshimi'. We identified 147 loci, which were expanded to 18 linkage groups with a total map length of 980 cM. Linkage analysis identified that both the double flower trait from 'Kirakiraboshi' (d Kira ) and the hortensia trait from 'Frau Yoshimi' (h Frau ) were located on linkage group KF_4. Detailed linkage analysis using 351 F 2 progeny revealed a 34.8 cM map length between the two loci and identified two tightly linked SSR markers, STAB045 for d Kira and HS071 for h Frau . Genetic analysis suggested that double flower and hortensia traits are each controlled by a single recessive gene. Together, the linkage map, SSR markers, and genetic information obtained in this study will be useful for future hydrangea breeding.

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Genome size variation and species relationships in the genus Hydrangea

Cited by 61 publications

References 26 publications

Pollen viability and meiotic behaviour in intraspecific hybrids of Hydrangea aspera subsp. aspera Kawakami group x subsp. sargentiana

Pollen viability and meiotic behaviour in intraspecific hybrids of Hydrangea aspera subsp. aspera Kawakami group x subsp. sargentiana

Cytogenetic characterization of Hydrangea involucrata Sieb. and H. aspera D. Don complex (Hydrangeaceae): genetic, evolutional, and taxonomic implications

Development of DNA Markers Linked to Double-Flower and Hortensia Traits in <i>Hydrangea macrophylla</i> (Thunb.) Ser.

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