Genome wide and evolutionary analysis of heat shock protein 70 proteins in tomato and their role in response to heat and drought stress

Ahmad, Muhammad Zulfiqar; Shah, Zamarud; Ullah, Arif; Ahmed, Shakeel; Ahmad, Bushra; Khan, Ilyas

doi:10.1007/s11033-022-07734-1

Cited by 5 publications

(5 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, several CsHsp70 genes displayed altered expression under drought and differential temperature-photoperiod environments. Similar results were also observed in previous reports, such as potato [10], tomato [13], pumpkin [15], and chrysanthemum [16], in which a large proportion of Hsp70 genes display inducibility in response to at least one or more abiotic stresses.…”

Section: Discussionsupporting

confidence: 91%

“…To explore the evolutionary relationships of the CsHsp70 proteins, a phylogenetic tree was generated using the protein sequences of the Hsp70 family members from 12 CsHsp70s in cucumber, 18 AtHsp70s in Arabidopsis [31], 32 OsHsp70s in rice [8], 21 CaHsp70s in pepper [9], 22 ZmHsp70s in maize [32], and 21 SlHsp70s in tomato [13]. The resulting phylogenetic tree revealed that the above Hsp70 proteins were clustered into four groups (A-D) based on the bootstrap support (Figure 1).…”

Section: Evolutionary Relationships Of Cshsp70 Proteinsmentioning

confidence: 99%

“…To identify Hsp70 family members in cucumber, we used the Hidden Maekov Model (HMM) profile of the Hsp70 domain (PF00012) acquired from the Pfam database (http://pfam.xfam.org/, accessed on 1 May 2023) for hmmsearch (HMMER version 3.3.2) against the Cucurbit Genomics Database (CuGenDB, http://cucurbitgenomics.org/, accessed on 1 May 2023). In addition, the full-length Hsp70 amino acid sequences from rice [8], pepper [9], and tomato [13], were also retrieved and used as query sequences to perform BLAST (basic local alignment search tool) search against the CuGenDB database. The resulting candidate cucumber Hsp70s were confirmed by SMART (http://smart.emblheidelberg.de/, accessed on 1 May 2023), for the presence of the Hsp70 domain (PF00012).…”

Section: Database Searches and Annotation Of The Hsp70 Members In Cuc...mentioning

confidence: 99%

“…Plant Hsp70 genes were composed of a multi-gene family and have been extensively identified in a variety of plants, such as rice [7,8], pepper [9], potato [10], tobacco [11], cabbage [12], tomato [13,14], pumpkin [15], chrysanthemum [16], and radish [17]. In previous studies, many recognized Hsp70 genes have been characterized to quickly express and accumulate under a number of abiotic stresses, such as heavy metal [14,18], salt [19], drought [20,21], heat [7,16], UV and high intensity light stress [22], as well as biotic stresses, such as pathogen and virus infection [23,24].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Comprehensive Genomic Analysis and Expression Profile of Hsp70 Gene Family Related to Abiotic and Biotic Stress in Cucumber

Zhou,

Xiao,

Zhao

et al. 2023

Horticulturae

View full text Add to dashboard Cite

Heat shock protein 70 (Hsp70) is a class of HSPs involved in plant growth and development, stress response and regulation. The Hsp70 proteins exist widely in the plant world, but the detail information about Hsp70s is still unclear in cucumber. Based on the available cucumber genome, a total of 12 Hsp70 genes (CsHsp70-1 to CsHsp70-12) were identified in this study, and they were distributed among five out of seven chromosomes. The CsHsp70s were divided into four groups based on a phylogenetic analysis by using protein sequences from cucumber and other plants, and their conserved motifs were relatively conserved. Gene duplication analysis showed that segmental duplication is the main driving force of expansion in cucumber CsHsp70 genes. Promoter analysis of CsHsp70 genes showed that they contained many cis-acting elements involved in hormone and stress responses. Expression analysis by RNA-seq and qRT-PCR indicated that the expression of most CsHsp70 genes was associated with multiple biotic and abiotic stresses in cucumber. This study introduces the characteristics of cucumber CsHsp70 genes and the regulation of their expression levels in various abiotic and biotic stresses, which provided a basis for functional exploration and utilization of CsHsp70 genes in the future.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Evolutionary Relationships Of Cshsp70 Proteinsmentioning

confidence: 99%

Section: Database Searches and Annotation Of The Hsp70 Members In Cuc...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comprehensive Genomic Analysis and Expression Profile of Hsp70 Gene Family Related to Abiotic and Biotic Stress in Cucumber

Zhou,

Xiao,

Zhao

et al. 2023

Horticulturae

View full text Add to dashboard Cite

show abstract

“…Several genes involved in the co-expression network interaction were related to abiotic stress tolerance. SlE2F/DP3 co-expressed genes such as SlHSP SlLRR, SlF-box-LRR, NAC domain gene and B3 DNA binding domain were reported against biotic and abiotic stress [ 19 , 34 , 63 , 64 ]. Similarly, another gene thymidylate synthase reported to control the expression of folate biosynthesis genes in tomato fruit was co-expressed with the SLE2F/DP 5 gene [ 65 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide characterization and expression profiling of E2F/DP gene family members in response to abiotic stress in tomato (Solanum lycopersicum L.)

Divya,

Robin,

Cho

et al. 2024

BMC Plant Biol

View full text Add to dashboard Cite

Background E2F/DP (Eukaryotic 2 transcription factor/dimerization partner) family proteins play an essential function in the cell cycle development of higher organisms. E2F/DP family genes have been reported only in a few plant species. However, comprehensive genome-wide characterization analysis of the E2F/DP gene family of Solanum lycopersicum has not been reported so far. Results This study identified eight nonredundant SlE2F/DP genes that were classified into seven groups in the phylogenetic analysis. All eight genes had a single E2F-TDP domain and few genes had additional domains. Two segmental duplication gene pairs were observed within tomato, in addition to cis-regulatory elements, miRNA target sites and phosphorylation sites which play an important role in plant development and stress response in tomato. To explore the three-dimensional (3D) models and gene ontology (GO) annotations of SlE2F/DP proteins, we pointed to their putative transporter activity and their interaction with several putative ligands. The localization of SlE2F/DP-GFP fused proteins in the nucleus and endoplasmic reticulum suggested that they may act in other biological functions. Expression studies revealed the differential expression pattern of most of the SlE2F/DP genes in various organs. Moreover, the expression of E2F/DP genes against abiotic stress, particularly SlE2F/DP2 and/or SlE2F/DP7, was upregulated in response to heat, salt, cold and ABA treatment. Furthermore, the co-expression analysis of SlE2F/DP genes with multiple metabolic pathways was co-expressed with defence genes, transcription factors and so on, suggested their crucial role in various biological processes. Conclusions Overall, our findings provide a way to understand the structure and function of SlE2F/DP genes; it might be helpful to improve fruit development and tolerance against abiotic stress through marker-assisted selection or transgenic approaches.

show abstract

Insilico-based genome-wide identification and structural characterization of heat shock proteins 70 (HSP70) in cowpea (Vigna unguiculata L.)

Shah,

Ahmad,

Ullah

et al. 2023

Genet Resour Crop Evol

View full text Add to dashboard Cite

Genome wide and evolutionary analysis of heat shock protein 70 proteins in tomato and their role in response to heat and drought stress

Cited by 5 publications

References 48 publications

Comprehensive Genomic Analysis and Expression Profile of Hsp70 Gene Family Related to Abiotic and Biotic Stress in Cucumber

Comprehensive Genomic Analysis and Expression Profile of Hsp70 Gene Family Related to Abiotic and Biotic Stress in Cucumber

Genome-wide characterization and expression profiling of E2F/DP gene family members in response to abiotic stress in tomato (Solanum lycopersicum L.)

Insilico-based genome-wide identification and structural characterization of heat shock proteins 70 (HSP70) in cowpea (Vigna unguiculata L.)

Contact Info

Product

Resources

About