Genome-wide association study for semen volume and total number of sperm in Holstein-Friesian bulls

Hering, Dorota; Oleński, Kamil; Ruść, A.; Kamiński, Stanisław

doi:10.1016/j.anireprosci.2014.10.022

Cited by 43 publications

(28 citation statements)

References 27 publications

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“…Spermatozoa account for approximately 1.0-1.5 mL of the raw semen (depending on sperm concentration) as observed from the pellet obtained after centrifugation. Using this rough estimation, we estimated that commercial semen doses from the bull stud contained approximately 0.8-12% (v/v) SP, which is in agreement with other reports (Hering et al 2014;Bromfield 2016). The purpose of the present study was to investigate the impact of adding bovine SP from bulls of high fertility and low fertility to SP-free ejaculated sperm samples before freezing on bull sperm quality after thawing.…”

Section: Introductionsupporting

confidence: 84%

Adding bovine seminal plasma prior to freezing improves post-thaw bull sperm kinematics but decreases mitochondrial activity

Nongbua

Al-Essawe

Edman³

et al. 2018

Systems Biology in Reproductive Medicine

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Section: Introductionsupporting

confidence: 84%

Adding bovine seminal plasma prior to freezing improves post-thaw bull sperm kinematics but decreases mitochondrial activity

Nongbua

Al-Essawe

Edman³

et al. 2018

Systems Biology in Reproductive Medicine

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“…A rough estimation of the amount of seminal plasma normally found in a bull semen dose can be obtained by assuming that the spermatozoa occupy a volume of 1.0–1.5 ml in raw semen (depending on the sperm concentration). Using this rough estimation, we found that commercial semen doses contain approximately 0.8%–12% SP (Bromfield, ; Hering, Oleński, Ruść, & Kaminski, ). The proportions of SP investigated here (75 μl and 300 μl) represent 1% and 4% of the cell culture medium; it is difficult to obtain a higher proportion in our cell culture system without depriving the cells of nutrients.…”

Section: Discussionmentioning

confidence: 99%

Effect of bovine seminal plasma on bovine endometrial epithelial cells in culture

Nongbua

Guo

Edman³

et al. 2017

Reprod Domestic Animals

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The purpose of this study was to investigate the effect of seminal plasma (SP) from bulls of known fertility on bovine endometrial epithelial cells (bEEC) in culture. The bEEC from passage 5, approximately 5.0-13 × 10 cells per flask, were challenged with SP from bulls of high or low fertility (n = 3 and 2, respectively) or PBS (control), at 1% (75 μl) or 4% (300 μl) and were incubated for 72 hr (n = 13 per challenge). Total cell number and viability of bEEC after challenge with 1% SP from either high- or low-fertility bulls (75H or 75L, respectively) did not differ from controls. In contrast, challenge with 4% of SP from high- or low-fertility bulls (300H or 300L) negatively affected bEEC cell number and viability. Challenge with 300 L had a greater adverse effect than 300H. These results suggest that the negative effect of bovine SP on bEEC is both dose-dependent and fertility-dependent.

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“…Fortes et al detected the most significant SNPs in the X chromosome associated with the percentage of progressive motile spermatozoa at 18 months of age and the percentage of normal spermatozoa at 24 months of age [6]. Hering et al also highlighted several candidate genes associated with sperm concentration, sperm motility and sperm volume in Holstein-Friesian populations [7–9]. In addition, some genes such as FSHR , INHA , TNP1 , TNP2 , CAPN1 and SPAG11 have been widely studied as candidate genes for semen production traits of bulls [10–14].…”

Section: Introductionmentioning

confidence: 99%

Genetic effects of PDGFRB and MARCH1 identified in GWAS revealing strong associations with semen production traits in Chinese Holstein bulls

Liu

Yin

et al. 2017

BMC Genet

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BackgroundUsing a genome-wide association study strategy, our previous study discovered 19 significant single-nucleotide polymorphisms (SNPs) related to semen production traits in Chinese Holstein bulls. Among them, three SNPs were within or close to the phosphodiesterase 3A (PDE3A), membrane associated ring-CH-type finger 1 (MARCH1) and platelet derived growth factor receptor beta (PDGFRB) genes. The present study was designed with the objectives of identifying genetic polymorphism of the PDE3A, PDGFRB and MARCH1 genes and their effects on semen production traits in a Holstein bull population.ResultsA total of 20 SNPs were detected and genotyped in 730 bulls. Association analyses using de-regressed estimated breeding values of each semen production trait revealed four statistically significant SNPs for one or more semen production traits (P < 0.05): one SNP was located downstream of PDGFRB and three SNPs were located in the promoter of MARCH1. Interestingly, for MARCH1, haplotype-based analysis revealed significant associations of haplotypes with semen volume per ejaculate. Furthermore, high expression of the MARCH1 gene was observed in sperm cells. One SNP (rs43445726) in the regulatory region of MARCH1 had a significant effect on gene expression.ConclusionOur study demonstrated the significant associations of genetic variants of the PDGFRB and MARCH1 genes with semen production traits. The identified SNPs may serve as genetic markers to optimize breeding programs for semen production traits in Holstein bull populations.Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-017-0527-1) contains supplementary material, which is available to authorized users.

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Genome-wide association study for semen volume and total number of sperm in Holstein-Friesian bulls

Cited by 43 publications

References 27 publications

Adding bovine seminal plasma prior to freezing improves post-thaw bull sperm kinematics but decreases mitochondrial activity

Adding bovine seminal plasma prior to freezing improves post-thaw bull sperm kinematics but decreases mitochondrial activity

Effect of bovine seminal plasma on bovine endometrial epithelial cells in culture

Genetic effects of PDGFRB and MARCH1 identified in GWAS revealing strong associations with semen production traits in Chinese Holstein bulls

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