Genome-wide characterization, evolution and expression profiling of UDP-glycosyltransferase family in pomelo (Citrus grandis) fruit

Abstract

Background: Pomelo is one of the three major species of citrus. The fruit accumulates a variety of abundant secondary metabolites that affect the flavor. UDP-glycosyltransferases (UGTs) are involved in the glycosylation of secondary metabolites. Results: In the present study, we performed a genome-wide analysis of pomelo UGT family, a total of 145 UGTs was identified based on the conserved plant secondary product glycosyltransferase (PSPG) motif. These UGT genes were clustered into 16 major groups through phylogenetic analysis of these genes with other plant UGTs (A-P). Pomelo UGTs were distributed unevenly among the chromosomes. At least 10 intron insertion events were observed in these UGT genome sequences, and I-5 was identified to be the highest conserved one. The expression profile analysis of pomelo UGT genes in different fruit tissues during development and ripening was carried out by RNA-seq. Conclusions: We identified 145 UGTs in pomelo fruit through transcriptome data and citrus genome database. Our research provides available information on UGTs studies in pomelo, and provides an important research foundation for screening and identification of functional UGT genes.

Genomic insights into longan evolution from a chromosome-level genome assembly and population genomics of longan accessions

“…A phylogenetic tree was constructed using UGT protein sequences in longan and other plants including Arabidopsis, Citrus (Figure 3e), in which 115 expanded UGTs were divided into ten groups. Five groups A, D, E, G, and L expanded more than others, although the number of genes in these groups varied widely among species 23 . In longan, group A, D, H, I, and L expanded more than the other groups.…”

“…InterPro (IPR) protein domain enrichment analysis showed that the longan expanded gene families are significantly enriched with IPR domains such as UGTs and cytochrome P450s (Supplementary Figure 3). Longan genome encodes more (215) UGTs (Supplementary Table 7) than Arabidopsis (107), C. grandis (145) and V. vinifera (181) do, but fewer than in apple does (241) 23 . UGTs also participate in plant development, growth and defense responses.…”

Phytochemical and Therapeutic Potential of Citrus grandis (L.) Osbeck: A Review

“…50 Citrus grandis can exhibit different therapeutic activity for the presence of particular class of compounds in aqueous, methanolic, and petroleum ether extract, which were indicated by phytochemical screening of the plant. 59,62,76 The ethanolic extract of mesocarp, pericarp and segment membrane of Citrus grandis fruit were prepared, where phenols (25.71 mg CE/100 ml), saponins (345.39 mg CE/100 ml), flavonoids (31.48 mg GAE/100 ml) and tanins (40.47 mg CE/100 ml) were found in the highest amount in the pericarp extract. In the ethanolic extract of segment membrane, the amounts of phenols, saponins, flavonoids and tanins were 18.46 (mg CE/100 ml), 147.93 (mg CE/100 ml), 20.17 (mg GAE/ 100 ml) and 21.02 (mg CE/100 ml) and in the mesocarp extract the amounts were 13.94 (mg CE/100 ml), 79.03 (mg CE/100 ml), 11.65 (mg GAE/100 ml)) and 7.94 (mg CE/100 ml) respectively.…”