Genome-Wide Identification and Expression Analysis of TUA and TUB Genes in Wheat (Triticum aestivum L.) during Its Development

Abstract

Microtubules play a fundamental role in plant development, morphogenesis, and cytokinesis; they are assembled from heterodimers containing an α-tubulin (TUA) and a β-tubulin (TUB) protein. However, little research has been conducted on the TUA and TUB gene families in hexaploid wheat (Triticum aestivum L.). In this study, we identified 15 TaTUA and 28 TaTUB genes in wheat. Phylogenetic analysis showed that 15 TaTUA genes were divided into two major subfamilies, and 28 TaTUB genes were divided into five major subfamilies. Mostly, there were similar motif compositions and exon-intron structures among the same subfamilies. Segmental duplication of genes (WGD/segmental) is the main process of TaTUA and TaTUB gene family expansion in wheat. It was found that TaTUA and TaTUB genes presented specific temporal and spatial characteristics based on the expression profiles of 17 tissues during wheat development using publicly available RNA-seq data. It was worth noting, via qRT-PCR, that two TaTUA and five TaTUB genes were highly expressed in fertile anthers compared to male sterility. These were quite different between physiological male sterile lines and S-type cytoplasmic male sterile lines at different stages of pollen development. This study offers fundamental information on the TUA and TUB gene families during wheat development and provides new insights for exploring the molecular mechanism of wheat male sterility.

Autophagy formation, microtubule disorientation, and alteration of ATG8 and tubulin gene expression under simulated microgravity in Arabidopsis thaliana

“… 68 , Salicaceae ( Populus trichocarpa , Salix arbutifolia ) 44 , 69 , Fabaceae ( Medicago truncatula ) 44 , 69 , Myrtaceae ( Eucalyptus grandis ) 68 , 69 , Linaceae ( Linum usitatissimum ) 67 , 70 , Malvacaeae ( Gossypium sp.) 44 , 71 and, possibly, in Poaceae ( Zea mays , Hordeum vulgare , Triticum aestivum , Oryza sativa ) 44 , 72 , 73 . However, this group of β-tubulins could be comprised out of the genes, orthologs of which are absent in A. thaliana genome.…”

Genome-wide identification and expression analysis of kinesin genes superfamily suggested their roles in response to abiotic stress and fertility of wheat (Triticum aestivum L.)

“…During gametogenesis, if division of microspore is abnormal, male sterility will appear. And abnormal division may be caused by abnormal microtubules [61]. In our research, CHA-MS1376, S-MS1376, K-MSYS9 and AS-MSYE9 wheat were used as the materials.…”