Genome-Wide Identification and Expression Analysis of the Cucumber FKBP Gene Family in Response to Abiotic and Biotic Stresses

Abstract

The FKBP (FK506-binding protein) gene family is an important member of the PPlase protease family and plays a vital role during the processes of plant growth and development. However, no studies of the FKBP gene family have been reported in cucumber. In this study, 19 FKBP genes were identified in cucumber, which were located on chromosomes 1, 3, 4, 6, and 7. Phylogenetic analysis divided the cucumber FKBP genes into three subgroups. The FKBP genes in the same subgroup exhibited similar structures and conserved motifs. The cis-acting elements analysis revealed that the promoters of cucumber FKBP genes contained hormone-, stress-, and development-related cis-acting elements. Synteny analysis of the FKBP genes among cucumber, Arabidopsis, and rice showed that 12 kinds of syntenic relationships were detected between cucumber and Arabidopsis FKBP genes, and 3 kinds of syntenic relationships were observed between cucumber and rice FKBP genes. The tissue-specific expression analysis showed that some FKBP genes were expressed in all tissues, while others were only highly expressed in part of the 10 types of tissues. The expression profile analysis of cucumber FKBP genes under 13 types of stresses showed that the CsaV3_1G007080 gene was differentially expressed under abiotic stresses (high temperature, NaCl, silicon, and photoperiod) and biotic stresses (downy mildew, green mottle mosaic virus, Fusarium wilt, phytophthora capsica, angular leaf spot, and root-knot nematode), which indicated that the CsaV3_1G007080 gene plays an important role in the growth and development of cucumber. The interaction protein analysis showed that most of the proteins in the FKBP gene family interacted with each other. The results of this study will lay the foundation for further research on the molecular biological functions of the cucumber FKBP gene family.

Identification and verification of a prognostic autophagy-related gene signature in hepatocellular carcinoma

“…FADD is inhibited in HCC, and its reduced expression inhibits its role in mediating liver cancer cell apoptosis 21 . FKBP1B is a member of the FKBP (FK506-binding protein) family and plays a critical role in plant growth and development 22 . This family of genes plays an inhibitory role in tumour progression, including the inhibitory effect of FKBP5 on breast cancer and the antiapoptotic effect of FKBP51 in melanoma 23 , 24 .…”

Combined Transcriptome and Proteome Analysis Reveals the Molecular Mechanism by Which ZmPDI Improves Salt Resistance in Rice (Oryza sativa)

“…FK506 binding proteins (FKBPs) gene family is an import group of peptidyl-prolyl isomerases, play roles in protein folding processes, and is involved in vital processes in a broad range of organisms, such as viruses, bacteria, fungi, plants, and animals [61,62]. The FKBPs form large protein families (23 isoforms in Arabidopsis thaliana [63], 24 in maize [64], and 29 in Oryza sativa [65]), and play roles in cell signaling, stress response, photosynthesis, and gene transcription.…”