Genome-wide identification and expression analysis of dirigent gene family in strawberry (Fragaria vesca) and functional characterization of FvDIR13

Shi, Yaqian; Shen, Yaru; Ahmad, Bilal; Yao, Liping; He, Tiannan; Fan, Jianshuai; Liu, Yuhan; Chen, Qing‐Xi; Wen, Zhi‐Hong

doi:10.1016/j.scienta.2022.110913

Cited by 11 publications

(5 citation statements)

References 60 publications

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“…Gene duplication, a key driver of speciation [39], enables organisms to adapt to changing environments [19]. In plants, tandem and segmental replication are the most common causes of gene family expansion [40], with segmental replication occurring regularly in slower-evolving gene families [41]. In wheat, 12 pairs of covarying JmjC family genes occur in the A, B, and D genomes, suggesting that JMJs have been highly conserved during wheat evolution and that there is gene redundancy between JMJs in different chromosome groups.…”

Section: Discussionmentioning

confidence: 99%

JmjC Domain-containing Histone Demethylase Gene Family in Chinese Cabbage: Genome-wide Identification and Expressional Profiling

YIN,

HU,

CAO

et al. 2023

Preprint

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The Jumonji C (JmjC) structural domain-containing gene family, an important family of histone demethylases in plants, has an essential role in plant growth and development, flowering transformation, and stress responses. Here, we aimed to elucidate its role in Chinese cabbage (Brassica rapa ssp. pekinensis). At the genome level, we identified 29 members of this family that have remained highly conserved. For these genes, the promoter cis-acting elements were enriched in responses to stress related to light and cold, anaerobic conditions, and phytohormone treatment. BrJMJs were highly expressed in the siliques and flowers, suggesting that histone demethylation might play a crucial role in reproductive organ growth. Stress associated with cadmium (Cd) exposure, drought, salinity, cold, and heat induced their expression. The expression of some of the genes (BrJMJ1/2/5/13/21/24) increased gradually with increasing Cd concentration. BrJMJ4 and BrJMJ29 were induced by drought, salt, cold, and heat stress. This work supports future analysis of the biological functions of JmjC family genes and elucidates the molecular mechanisms of abiotic stress responses in Chinese cabbage.

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Section: Discussionmentioning

confidence: 99%

JmjC Domain-containing Histone Demethylase Gene Family in Chinese Cabbage: Genome-wide Identification and Expressional Profiling

YIN,

HU,

CAO

et al. 2023

Preprint

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“…Pathogen inoculation ( C. gloeosporioides and C. higginsianum ) and plant maintenance were carried out according to our previous experiment [ 53 ]. For the purpose of determining the biological efficacy of FvChi-14 against anthracnose, C. higginsianum was used to infect both A. thaliana transgenic lines and the Col-0 wild types [ 54 ].…”

Section: Methodsmentioning

confidence: 99%

Bioinformatics and Expression Analysis of the Chitinase Genes in Strawberry (Fragaria vesca) and Functional Study of FvChi-14

Fan

Jiao

et al. 2023

Plants

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Plant chitinases (EC 3.2.1.14) are pathogenesis-related (PR) proteins and are well studied in many plant species. However, little is known about the genomic organization and expression of chitinase genes in strawberries (Fragaria vesca). Here, 23 FvChi genes were identified in the genome of strawberry (F. vesca) and divided into GH18 and GH19 subfamilies based on phylogenetic relationships. A detailed bioinformatics analysis of the FvChi genes was performed, including gene physicochemical properties, chromosomal location, exon–intron distribution, domain arrangement, and subcellular localization. Twenty-two FvChi genes showed upregulation after Colletotrichum gloeosporioides infection. Following the exogenous application of SA, FvChi-3, 4, and 5 showed significant changes in expression. The ectopic expression of FvChi-14 in Arabidopsis thaliana increased resistance to C. higginsianum via controlling the SA and JA signaling pathway genes (AtPR1, AtICS1, AtPDF1.2, and AtLOX3). The FvChi-14 protein location was predicted in the cell wall or extracellular matrix. We speculate that FvChi-14 is involved in disease resistance by regulating the SA and JA signaling pathways. The findings of this study provide a theoretical reference for the functional studies of FvChi genes and new candidates for strawberry stress resistance breeding programs.

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“…The empty vector was also transformed as a control. After two days of dark culture, the GFP (Green Fluorescent Protein) fluorescence response in the onion epidermis was observed using laser scanning confocal microscopy (OLYMPUS IX83-FV3000) [36].…”

Section: Subcellular Localization Of Fvtrihelix6mentioning

confidence: 99%

“…The leaves were removed after sufficient staining, submerged in 90% ethanol, and placed in boiling water until the green color faded. This was repeated 3-5 times using 8-10 tobacco leaves each time [36,38]. Agrobacterium suspension containing the pFvTrihelix6::GUS fusion vector was injected.…”

Section: Cloning Of Fvtrihelix6 Promoter Gus Protein Staining and Gus...mentioning

confidence: 99%

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Expression Analysis of Trihelix Transcription Factor Family in Strawberries and Functional Characterization of FvTrihelix6

Fan

Sun

et al. 2023

Horticulturae

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The Trihelix is a plant-specific transcription factor family and has critical roles in plant growth and development and stress resistance. There is less information about Trihelix transcription factor genes and their potential functions in strawberries (Fragaria vesca). In the present study, we performed a detailed bioinformatics analysis of the Trihelix family in strawberries including physicochemical properties, chromosomal location, exon–intron distribution, domain arrangement, and subcellular localization. Thirty Trihelix family members were identified and divided into five subfamilies. The expression of FvTrihelix genes in different tissues/organs, i.e., root, stolon, leaf, flower, and fruit, was measured in strawberries after infection with Colletotrichum. gloeosporioides and foliar applications of salicylic acid (SA) and jasmonic acid (JA). Most of the genes showed differential expression responses following C. gloeosporioides infection and hormone treatments (SA and JA), suggesting critical roles in disease resistance and hormonal signaling pathways. As anticipated, the ectopic expression of FvTrihelix6 in Arabidopsis thaliana increased resistance against Colletotrichum. higginsianum infection. FvTrihelix6 protein was localized in the nucleus. We surmise that FvTrihelix6 enhances resistance against pathogens through the SA and JA signaling pathways. This study provides novel insights into the strawberry Trihelix transcription factor genes and new candidates for disease-resistance breeding of strawberries.

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Genome-wide identification and expression analysis of dirigent gene family in strawberry (Fragaria vesca) and functional characterization of FvDIR13

Cited by 11 publications

References 60 publications

JmjC Domain-containing Histone Demethylase Gene Family in Chinese Cabbage: Genome-wide Identification and Expressional Profiling

JmjC Domain-containing Histone Demethylase Gene Family in Chinese Cabbage: Genome-wide Identification and Expressional Profiling

Bioinformatics and Expression Analysis of the Chitinase Genes in Strawberry (Fragaria vesca) and Functional Study of FvChi-14

Expression Analysis of Trihelix Transcription Factor Family in Strawberries and Functional Characterization of FvTrihelix6

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