Genome-wide identification and expression analysis of flowering-related genes reveal putative floral induction and differentiation mechanisms in tea plant (Camellia sinensis)

Li, Ying; Hao, Xinyuan; Lu, Qinhua; Zhang, Weifu; Zhang, Haojie; Wang, Lu; Yang, Yajun; Xiao, Bin; Wang, Xinchao

doi:10.1016/j.ygeno.2020.01.003

Cited by 28 publications

(39 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Hormone Signaling: PGR Treatments Modulate GA and ABA Signaling Cassava homologs of the Arabidopsis transcripts Gibberellin-Insensitive Dwarf1b (GID1b) and Gibberellin 2-oxidase2 (GA2ox2) were downregulated by PGR treatments (Figures 9, 10). In some species, such as tea (Camellia sinensis) and Magnolia x soulangeana, GID genes are expressed at elevated levels when floral induction takes place (Jiang et al, 2020;Liu et al, 2020). GA2 oxidases, on the other hand, inactivate bioactive gibberellins (Thomas et al, 1999).…”

Section: Regulation Of Gene Expression With Pruning Combined With Sts+bamentioning

confidence: 99%

Flower Development in Cassava Is Feminized by Cytokinin, While Proliferation Is Stimulated by Anti-Ethylene and Pruning: Transcriptome Responses

et al. 2021

View full text Add to dashboard Cite

Cassava, a tropical storage-root crop, is a major source of food security for millions in the tropics. Cassava breeding, however, is hindered by the poor development of flowers and a low ratio of female flowers to male flowers. To advance the understanding of the mechanistic factors regulating cassava flowering, combinations of plant growth regulators (PGRs) and pruning treatments were examined for their effectiveness in improving flower production and fruit set in field conditions. Pruning the fork-type branches, which arise at the shoot apex immediately below newly formed inflorescences, stimulated inflorescence and floral development. The anti-ethylene PGR silver thiosulfate (STS) also increased flower abundance. Both pruning and STS increased flower numbers while having minimal influence on sex ratios. In contrast, the cytokinin benzyladenine (BA) feminized flowers without increasing flower abundance. Combining pruning and STS treatments led to an additive increase in flower abundance; with the addition of BA, over 80% of flowers were females. This three-way treatment combination of pruning+STS+BA also led to an increase in fruit number. Transcriptomic analysis of gene expression in tissues of the apical region and developing inflorescence revealed that the enhancement of flower development by STS+BA was accompanied by downregulation of several genes associated with repression of flowering, including homologs of TEMPRANILLO1 (TEM1), GA receptor GID1b, and ABA signaling genes ABI1 and PP2CA. We conclude that flower-enhancing treatments with pruning, STS, and BA create widespread changes in the network of hormone signaling and regulatory factors beyond ethylene and cytokinin.

show abstract

Section: Regulation Of Gene Expression With Pruning Combined With Sts+bamentioning

confidence: 99%

Flower Development in Cassava Is Feminized by Cytokinin, While Proliferation Is Stimulated by Anti-Ethylene and Pruning: Transcriptome Responses

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Armstead et al [ 39 ] reported a genetic co-spectral region between rice and ryegrass, including the HD3 heading-date QTL and a major QTL of rice, accounted for 70% variation. In addition, newly developed markers related the other flowering candidate genes in this experiment have been reported in other species, such as ELF3 , GID1 , GA2OX6 , XCT , and GIGANTEA [ 40 , 41 , 42 , 43 , 44 ]. In future studies, we will explore the function and regulatory mechanism of these genes in E. sibiricus by studying the effect of nucleotide variation of these genes on flowering by sequencing of candidate gene-based markers.…”

Section: Discussionmentioning

confidence: 84%

Development of EST-SSR Markers Linked to Flowering Candidate Genes in Elymus sibiricus L. Based on RNA Sequencing

Zheng

Zhang

Wan

et al. 2020

Plants

View full text Add to dashboard Cite

Elymus sibiricus L. is an important cold-season grass with excellent cold and drought tolerance, good palatability, and nutrition. Flowering time is a key trait that affects forage and seed yield. Development of EST-SSR (expressed sequence tag simple sequence repeat) markers based on flowering genes contributes to the improvement of flowering traits. In the study, we detected 155 candidate genes related to flowering traits from 10,591 unigenes via transcriptome sequencing in early- and late-flowering genotypes. These candidate genes were mainly involved in the photoperiodic pathway, vernalization pathway, central integrator, and gibberellin pathway. A total of 125 candidate gene-based EST-SSRs were developed. Further, 15 polymorphic EST-SSRs closely associated to 13 candidate genes were used for genetic diversity and population structure analysis among 20 E. sibiricus accessions, including two contrasting panels (early-flowering and late-flowering). Among them, primer 28366, designed from heading date 3a (HD3a), effectively distinguished early- and late-flowering genotypes using a specifically amplified band of 175 bp. The polymorphic information content (PIC) value ranged from 0.12 to 0.48, with an average of 0.25. The unweighted pair group method analysis (UPGMA) cluster and structure analysis showed that the 20 E. sibiricus genotypes with similar flowering times tended to group together. These newly developed EST-SSR markers have the potential to be used for molecular markers assisted selection and germplasm evaluation of flowering traits in E. sibiricus.

show abstract

“…For example, serine/threonine-protein kinase SAPK2 ( c85598.graph_c0 ), protein ethylene insensitive 3 EIN3 ( c87915.graph_c0 ), and gibberellin receptor GID1B ( c82782.graph_c0 and c88437.graph_c0 ) were observed to be upregulated under the NaCl treated samples of V11E0022. Previously conducted studies also showed the upregulation of SAPK2 , EIN3 , and GID1B in the leaves of rice, tea plant, and Arabidopsis, respectively, under salt and drought stress [ 94 , 95 , 96 ]. The overexpression of SAPK2 supported rice to stand against salt stress.…”

Section: Discussionmentioning

confidence: 99%

Investigation of an Antioxidative System for Salinity Tolerance in Oenanthe javanica

Kumar

Yang

et al. 2020

Antioxidants

View full text Add to dashboard Cite

Abiotic stress, such as drought and salinity, severely affect the growth and yield of many plants. Oenanthe javanica (commonly known as water dropwort) is an important vegetable that is grown in the saline-alkali soils of East Asia, where salinity is the limiting environmental factor. To study the defense mechanism of salt stress responses in water dropwort, we studied two water dropwort cultivars, V11E0022 and V11E0135, based on phenotypic and physiological indexes. We found that V11E0022 were tolerant to salt stress, as a result of good antioxidant defense system in the form of osmolyte (proline), antioxidants (polyphenols and flavonoids), and antioxidant enzymes (APX and CAT), which provided novel insights for salt-tolerant mechanisms. Then, a comparative transcriptomic analysis was conducted, and Gene Ontology (GO) analysis revealed that differentially expressed genes (DEGs) involved in the carbohydrate metabolic process could reduce oxidative stress and enhance energy production that can help in adaptation against salt stress. Similarly, lipid metabolic processes can also enhance tolerance against salt stress by reducing the transpiration rate, H2O2, and oxidative stress. Furthermore, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that DEGs involved in hormone signals transduction pathway promoted the activities of antioxidant enzymes and reduced oxidative stress; likewise, arginine and proline metabolism, and flavonoid pathways also stimulated the biosynthesis of proline and flavonoids, respectively, in response to salt stress. Moreover, transcription factors (TFs) were also identified, which play an important role in salt stress tolerance of water dropwort. The finding of this study will be helpful for crop improvement under salt stress.

show abstract

Genome-wide identification and expression analysis of flowering-related genes reveal putative floral induction and differentiation mechanisms in tea plant (Camellia sinensis)

Cited by 28 publications

References 61 publications

Flower Development in Cassava Is Feminized by Cytokinin, While Proliferation Is Stimulated by Anti-Ethylene and Pruning: Transcriptome Responses

Flower Development in Cassava Is Feminized by Cytokinin, While Proliferation Is Stimulated by Anti-Ethylene and Pruning: Transcriptome Responses

Development of EST-SSR Markers Linked to Flowering Candidate Genes in Elymus sibiricus L. Based on RNA Sequencing

Investigation of an Antioxidative System for Salinity Tolerance in Oenanthe javanica

Contact Info

Product

Resources

About