Genome-Wide Identification and Expression Analysis of Rosa roxburghii Autophagy-Related Genes in Response to Top-Rot Disease

Abstract

Autophagy is a highly conserved process in eukaryotes that degrades and recycles damaged cells in plants and is involved in plant growth, development, senescence, and resistance to external stress. Top-rot disease (TRD) in Rosa roxburghii fruits caused by Colletotrichum fructicola often leads to huge yield losses. However, little information is available about the autophagy underlying the defense response to TRD. Here, we identified a total of 40 R. roxburghii autophagy-related genes (RrATGs), which were highly homologous to Arabidopsis thaliana ATGs. Transcriptomic data show that RrATGs were involved in the development and ripening processes of R. roxburghii fruits. Gene expression patterns in fruits with different degrees of TRD occurrence suggest that several members of the RrATGs family responded to TRD, of which RrATG18e was significantly up-regulated at the initial infection stage of C. fructicola. Furthermore, exogenous calcium (Ca2+) significantly promoted the mRNA accumulation of RrATG18e and fruit resistance to TRD, suggesting that this gene might be involved in the calcium-mediated TRD defense response. This study provided a better understanding of R. roxburghii autophagy-related genes and their potential roles in disease resistance.

Comprehensive Analysis of Autophagy-Related Genes in Rice Immunity against Magnaporthe oryzae

“…For example, all OsATG8 genes contained the "ubl-ATG8" domain and motif 1, while all OsATG18 genes contained the "WD40" domain and motif 3 (Figures 2b and 3a). These analytical results show similarities to the analysis of ATG homologs in other species [11,30].…”

“…The first ATG was identified in Saccharomyces cerevisiae, and since then, over 40 ATGs have been identified in eukaryotic organisms [6,7]. In various plant model organisms, such as Arabidopsis thaliana, Zea mays, Oryza sativa, Rosa roxburghii, and Camellia sinensis, ATGs have been extensively studied and found to be involved in growth, development, senescence, stress response, and immunity [8][9][10][11].…”

Dissipation kinetics, residue level, and risk assessment of chlorantraniliprole in Rosa roxburghii and its residue removal using household decontamination technique

“…The commercial formulation 200 gL -1 CAP suspension concentrate (SC) was obtained from FMC Corporation well as antioxidant, anti-aging, and anti-radiation effects (Luo et al, 2023;Wang et al, 2019;Zhang et al, 2022b). Early studies have shown that R. roxburghii juice promotes the formation of foam cells, reduce the accumulation of cholesterol in cells, and thus reduce the incidence of atherosclerosis (Zhang et al, 2001).…”