Genome-wide identification and expression characterization of ABCC-MRP transporters in hexaploid wheat

Abstract

The ABCC multidrug resistance associated proteins (ABCC-MRP), a subclass of ABC transporters are involved in multiple physiological processes that include cellular homeostasis, metal detoxification, and transport of glutathione-conjugates. Although they are well-studied in humans, yeast, and Arabidopsis, limited efforts have been made to address their possible role in crop like wheat. In the present work, 18 wheat ABCC-MRP proteins were identified that showed the uniform distribution with sub-families from rice and Arabidopsis. Organ-specific quantitative expression analysis of wheat ABCC genes indicated significantly higher accumulation in roots (TaABCC2, TaABCC3, and TaABCC11 and TaABCC12), stem (TaABCC1), leaves (TaABCC16 and TaABCC17), flag leaf (TaABCC14 and TaABCC15), and seeds (TaABCC6, TaABCC8, TaABCC12, TaABCC13, and TaABCC17) implicating their role in the respective tissues. Differential transcript expression patterns were observed for TaABCC genes during grain maturation speculating their role during seed development. Hormone treatment experiments indicated that some of the ABCC genes could be transcriptionally regulated during seed development. In the presence of Cd or hydrogen peroxide, distinct molecular expression of wheat ABCC genes was observed in the wheat seedlings, suggesting their possible role during heavy metal generated oxidative stress. Functional characterization of the wheat transporter, TaABCC13 a homolog of maize LPA1 confirms its role in glutathione-mediated detoxification pathway and is able to utilize adenine biosynthetic intermediates as a substrate. This is the first comprehensive inventory of wheat ABCC-MRP gene subfamily.

Expression of Genes Involved in Heavy Metal Trafficking in Plants Exposed to Salinity Stress and Elevated Cd Concentrations

“…It was demonstrated that Cd treatment did not affect A. thaliana abcc1 and abbc2 gene expression [45]; however, it enhanced the abcc3 transcript level. Recently, similar abcc1 and abcc2 responses to Cd were also confirmed for rapeseed and wheat [46,47]. Our findings agree with the above-mentioned findings and support the idea of the lack of heavy metal involvement in halophyte abcc2 expression regulation.…”

ABC Transporter-Mediated Transport of Glutathione Conjugates Enhances Seed Yield and Quality in Chickpea

“…The yeast mutant complementation assay suggested that the function of the CaABCC3(6) gene was similar to that of the yeast YCF1 and thus could help in GSconjugate transportation and detoxification (Li et al, 1996;Lu et al, 1998;Tommasini et al, 1998;Bhati et al, 2014Bhati et al, , 2015. The high-SW haplotype HAP C efficiently complemented the Δycf1 mutant strain (sensitive for cadmium treatment).…”

“…Wild-type parent yeast strain YPH299 and Δycf1, a known vacuolar GHS S-conjugate transporter mutant, were used for the complementation assay of low-and high-SW ABC transporter gene haplotypes. Two different constructs, comprising low-and high-SW ABC transporter gene haplotypes cloned in pYES260, were used to transform the Δycf1 mutant strain for the complementation assay following the experimental strategies described by Bhati et al (2014Bhati et al ( , 2015.…”

Tissue specific transcript profiling of wheat phosphate transporter genes and its association with phosphate allocation in grains

“…The in-silico identification of PHT genes from wheat was adapted from the approach reported previously52. The protein sequences of PHT family genes (PHT1, 2, 3 and 4) from Arabidospsis and rice (Supplementary Table 3) were queried against wheat expressed sequence tags (EST) database in tBLASTn program at NCBI (http://blast.ncbi.nlm.nih.gov/blast/Blast.cgi).…”