Genome-wide identification and expression pattern analysis of lipoxygenase genes of chickpea (Cicer arietinum L.) in response to accelerated aging

Malviya, Rinku; Dey, Sharmistha; Pandey, Anuradha; Gayen, Dipak

doi:10.1016/j.gene.2023.147482

Cited by 4 publications

(3 citation statements)

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“…The lipoxygenases are non-heme iron-containing enzymes that catalyze the oxygenation of fatty acids in plants. LOX genes have been categorized as 13S-LOX and 9S-LOX and are vital to plant defense through synthesis of Jasmonic acid [ 37 , 38 ], production of flavor profile in plants [ 39 – 41 ] fruit ripening [ 42 ], biotic stress resistance [ 43 ] and germination of seed [ 45 ]. LOX activity during storage leads to enhanced peroxidation and reduced nutritional quality and vigor, especially in lipid-rich seeds like soybean, chickpea etc [ 44 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

“…LOX genes have been categorized as 13S-LOX and 9S-LOX and are vital to plant defense through synthesis of Jasmonic acid [ 37 , 38 ], production of flavor profile in plants [ 39 – 41 ] fruit ripening [ 42 ], biotic stress resistance [ 43 ] and germination of seed [ 45 ]. LOX activity during storage leads to enhanced peroxidation and reduced nutritional quality and vigor, especially in lipid-rich seeds like soybean, chickpea etc [ 44 , 45 ]. Similarly, phospholipase D genes are also actively involved in seed germination, stress response, root system architecture and nutrient uptake, pollen tube growth, stomatal movement, rhizobium-root interactions, lipid signaling and oil rancidity [ 8 , 15 , 38 , 46 , and 47 ].…”

Section: Discussionmentioning

confidence: 99%

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Exploring selection signatures in the divergence and evolution of lipid droplet (LD) associated genes in major oilseed crops

Parakkunnel,

Vanishree

et al. 2024

BMC Genomics

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Background Oil bodies or lipid droplets (LDs) in the cytosol are the subcellular storage compartments of seeds and the sites of lipid metabolism providing energy to the germinating seeds. Major LD-associated proteins are lipoxygenases, phospholipaseD, oleosins, TAG-lipases, steroleosins, caleosins and SEIPINs; involved in facilitating germination and enhancing peroxidation resulting in off-flavours. However, how natural selection is balancing contradictory processes in lipid-rich seeds remains evasive. The present study was aimed at the prediction of selection signatures among orthologous clades in major oilseeds and the correlation of selection effect with gene expression. Results The LD-associated genes from the major oil-bearing crops were analyzed to predict natural selection signatures in phylogenetically close-knit ortholog clusters to understand adaptive evolution. Positive selection was the major force driving the evolution and diversification of orthologs in a lineage-specific manner. Significant positive selection effects were found in 94 genes particularly in oleosin and TAG-lipases, purifying with excess of non-synonymous substitution in 44 genes while 35 genes were neutral to selection effects. No significant selection impact was noticed in Brassicaceae as against LOX genes of oil palm. A heavy load of deleterious mutations affecting selection signatures was detected in T-lineage oleosins and LOX genes of Arachis hypogaea. The T-lineage oleosin genes were involved in mainly anther, tapetum and anther wall morphogenesis. In Ricinus communis and Sesamum indicum > 85% of PLD genes were under selection whereas selection pressures were low in Brassica juncea and Helianthus annuus. Steroleosin, caleosin and SEIPINs with large roles in lipid droplet organization expressed mostly in seeds and were under considerable positive selection pressures. Expression divergence was evident among paralogs and homeologs with one gene attaining functional superiority compared to the other. The LOX gene Glyma.13g347500 associated with off-flavor was not expressed during germination, rather its paralog Glyma.13g347600 showed expression in Glycine max. PLD-α genes were expressed on all the tissues except the seed,δ genes in seed and meristem while β and γ genes expressed in the leaf. Conclusions The genes involved in seed germination and lipid metabolism were under strong positive selection, although species differences were discernable. The present study identifies suitable candidate genes enhancing seed oil content and germination wherein directional selection can become more fruitful.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Exploring selection signatures in the divergence and evolution of lipid droplet (LD) associated genes in major oilseed crops

Parakkunnel,

Vanishree

et al. 2024

BMC Genomics

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“…Transcription factor (TF) binding sites prediction in the GmCYPs promoter region was executed using PlantTFDB online server (http://planttfdb.gao-lab.org/); using default parameters and p-value of ≤ 1e-6 (Malviya et al, 2023).…”

Section: Analysis Of Cis-acting Regulatory Elements Analysis In Gene ...mentioning

confidence: 99%

Comprehensive and evolutionary analysis of Spodoptera litura-inducible Cytochrome P450 monooxygenase gene family in Glycine max elucidate their role in defense

Yadav,

Panwar,

Rustagi

et al. 2023

Front. Plant Sci.

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Plants being sessile organisms and lacking both circulating phagocytic cells and somatic adaptive immune response, have thrived on various defense mechanisms to fend off insect pests and invasion of pathogens. CYP450s are the versatile enzymes, which thwart plants against insect pests by ubiquitous biosynthesis of phytohormones, antioxidants, and secondary metabolites, utilizing them as feeding deterrents and direct toxins. Therefore, a comprehensive analysis of biotic stress-responsive CYPs from Glycine max was performed to ascertain their function against S. litura-infestation. Phylogenetic analysis and evolutionary studies on conserved domains and motifs disclosed the evolutionary correspondence of these GmCYPs with already characterized members of the CYP450 superfamily and close relatedness to Medicago truncatula. These GmCYPs were mapped on 13 chromosomes; they possess 1-8 exons; they have evolved due to duplication and are localized in endoplasmic reticulumn. Further, identification of methyl-jasmonate, salicylic acid, defense responsive and flavonoid biosynthesis regulating cis-acting elements, their interaction with biotic stress regulating proteins and their differential expression in diverse types of tissues, and during herbivory, depicted their responsiveness to biotic stress. Three-dimensional homology modelling of GmCYPs, docking with heme cofactor required for their catalytic activity and enzyme-substrate interactions were performed to understand the functional mechanism of their action. Moreover, to gain insight into their involvement in plant defense, gene expression analysis was evaluated, which revealed differential expression of 11 GmCYPs upon S. litura-infestation, 12 GmCYPs on wounding while foliar spray of ethylene, methyl-jasmonate and salicylic acid differentially regulated 11 GmCYPs, 6 GmCYPs, and 10 GmCYPs respectively. Our study comprehensively analysed the underlying mechanism of GmCYPs function during S. litura-infestation, which can be further utilized for functional characterization to develop new strategies for enhancing soybean resistance to insect pests.

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Lipoxygenases (LOXs): Will turning off this genetic switch help safeguard the flavor and nutritional quality of stored lipid-rich staple foods?

Mathavaraj,

Muthusamy,

Katral

et al. 2025

Food Chemistry

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Genome-wide identification and expression pattern analysis of lipoxygenase genes of chickpea (Cicer arietinum L.) in response to accelerated aging

Cited by 4 publications

References 54 publications

Exploring selection signatures in the divergence and evolution of lipid droplet (LD) associated genes in major oilseed crops

Exploring selection signatures in the divergence and evolution of lipid droplet (LD) associated genes in major oilseed crops

Comprehensive and evolutionary analysis of Spodoptera litura-inducible Cytochrome P450 monooxygenase gene family in Glycine max elucidate their role in defense

Lipoxygenases (LOXs): Will turning off this genetic switch help safeguard the flavor and nutritional quality of stored lipid-rich staple foods?

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