Genome-Wide Identification and Expression Profile of OSCA Gene Family Members in Triticum aestivum L.

Abstract

Hyperosmolality and various other stimuli can trigger an increase in cytoplasmic-free calcium concentration ([Ca2+]cyt). Members of the Arabidopsis thaliana (L.) reduced hyperosmolality-gated calcium-permeable channels (OSCA) gene family are reported to be involved in sensing extracellular changes to trigger hyperosmolality-induced [Ca2+]cyt increases and controlling stomatal closure during immune signaling. Wheat (Triticum aestivum L.) is a very important food crop, but there are few studies of its OSCA gene family members. In this study, 42 OSCA members were identified in the wheat genome, and phylogenetic analysis can divide them into four clades. The members of each clade have similar gene structures, conserved motifs, and domains. TaOSCA genes were predicted to be regulated by cis-acting elements such as STRE, MBS, DRE1, ABRE, etc. Quantitative PCR results showed that they have different expression patterns in different tissues. The expression profiles of 15 selected TaOSCAs were examined after PEG (polyethylene glycol), NaCl, and ABA (abscisic acid) treatment. All 15 TaOSCA members responded to PEG treatment, while TaOSCA12/-39 responded simultaneously to PEG and ABA. This study informs research into the biological function and evolution of TaOSCA and lays the foundation for the breeding and genetic improvement of wheat.

Genome-Wide Identification, Evolution and Expressional Analysis of OSCA Gene Family in Barley (Hordeum vulgare L.)

“…In plants, several calcium channels have been characterized to find that they not only function to mediate the response to abiotic and biotic stresses, but are also involved in growth regulation and biosynthesis [ 12 , 13 , 14 , 15 , 16 , 17 ]. Among them, the hyperosmolality-gated calcium-permeable channel family ( OSCA ) is one of the most important and well-studied calcium channels participating in osmotic adjustment [ 18 , 19 ]. In light of its significance, the OSCA family has been identified in many plant species, including 15 OSCA members found in A. thaliana , 11 in rice [ 18 ], 42 in wheat [ 19 ], 12 in maize [ 20 , 21 ], 13 in mung bean [ 22 ], as well as 35 in cotton [ 23 ].…”

“…Among them, the hyperosmolality-gated calcium-permeable channel family ( OSCA ) is one of the most important and well-studied calcium channels participating in osmotic adjustment [ 18 , 19 ]. In light of its significance, the OSCA family has been identified in many plant species, including 15 OSCA members found in A. thaliana , 11 in rice [ 18 ], 42 in wheat [ 19 ], 12 in maize [ 20 , 21 ], 13 in mung bean [ 22 ], as well as 35 in cotton [ 23 ]. The expression profile of OSCA genes were also systematically investigated under diverse abiotic stresses in different plant species, which demonstrated their differential roles in stress response and tolerance [ 18 , 19 , 20 , 21 , 22 , 23 ].…”

“…In light of its significance, the OSCA family has been identified in many plant species, including 15 OSCA members found in A. thaliana , 11 in rice [ 18 ], 42 in wheat [ 19 ], 12 in maize [ 20 , 21 ], 13 in mung bean [ 22 ], as well as 35 in cotton [ 23 ]. The expression profile of OSCA genes were also systematically investigated under diverse abiotic stresses in different plant species, which demonstrated their differential roles in stress response and tolerance [ 18 , 19 , 20 , 21 , 22 , 23 ]. Some plant OSCA genes were also functionally validated.…”

Identification and Expression Profile Analysis of the OSCA Gene Family Related to Abiotic and Biotic Stress Response in Cucumber

“…OSCA proteins are distributed as multigene families in eukaryotic species possessing conserved DUF221 (Domain of Unknown Function 221) with 11 transmembrane helices and a cytosolic soluble domain that forms a homodimer of two pores [ 12 , 13 , 14 ]. Genome-wide survey and identification of the OSCA gene family have been widely conducted in different species, and the gene number ranged from 11 members in rice to 42 members in wheat [ 10 , 15 , 16 , 17 ]. In recent years, many reports have indicated the roles of OSCA genes in osmotic-related abiotic stress response.…”

“…Unfortunately, it often suffers from various biotic stresses (such as powdery mildew and downy mildew) and abiotic stress (drought, cold, osmotic, and salt) under natural conditions, which seriously affect its yield and quality [ 22 , 23 ]. The OSCA family has been investigated in many plant species, such as Arabidopsis [ 10 ], rice [ 15 ], maize [ 18 , 24 ], and wheat [ 17 ], whereas the members of OSCA family in cucumber have not been identified. In this study, we systematically identified the OSCA gene family members and analyzed their sequence characteristics, promoter regions, and tissue expression patterns.…”