Genome-wide identification and expression profiling analysis of Wnt family genes affecting adipocyte differentiation in cattle

Pan, Cuili; Wang, Shuzhe; Yang, Chaoyun; Hu, Chun‐Li; Sheng, Hui; Xue, Xiaoshu; Hu, Honghong; Lei, Zhaoxiong; Yang, Mengli; Ma, Yun

doi:10.1038/s41598-021-04468-1

Cited by 10 publications

(10 citation statements)

References 64 publications

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“…The other gene, FZD5 is a protein coding gene, which functions as transmembrane receptor and mediates Wnt ligands binding in Wnt signaling pathway and plays role in the control of tissue regeneration, cell proliferation and cell differentiation 49 , 50 . One recent study has proposed that the activation of FZD5 by Wnt protein could potentially play a role in regulating adipose differentiation 49 . A previous GWAS study identified FZD5 as a candidate gene associated with fertility traits in Beef Heifers 51 .…”

Section: Discussionmentioning

confidence: 99%

A transcriptomic analysis of skeletal muscle tissues reveals promising candidate genes and pathways accountable for different daily weight gain in Hanwoo cattle

Sheet,

Jang,

Kim

et al. 2024

Sci Rep

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Cattle traits like average daily weight gain (ADG) greatly impact profitability. Selecting based on ADG considering genetic variability can lead to economic and genetic advancements in cattle breeding. This study aimed to unravel genetic influences on ADG variation in Hanwoo cattle at the skeletal muscle transcriptomic level. RNA sequencing was conducted on longissimus dorsi (LD), semimembranosus (SB), and psoas major (PM) muscles of 14 steers assigned to same feed, grouped by low (≤ 0.71 kg) and high (≥ 0.77 kg) ADG. At P ≤ 0.05 and log2fold > 1.5, the distinct pattern of gene expression was identified with 184, 172, and 210 differentially expressed genes in LD, SB, and PM muscles, respectively. Tissue-specific responses to ADG variation were evident, with myogenesis and differentiation associated JAK-STAT signaling pathway and prolactin signaling pathways enriched in LD and SB muscles, while adipogenesis-related PPAR signaling pathways were enriched in PM muscle. Key hub genes (AXIN2, CDKN1A, MYC, PTGS2, FZD5, SPP1) were upregulated and functionally significant in muscle growth and differentiation. Notably, DPP6, CDKN1A, and FZD5 emerged as possible candidate genes linked to ADG variation. These findings enhance our understanding of genetic factors behind ADG variation in Hanwoo cattle, illuminating skeletal muscle mechanisms influencing ADG.

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Section: Discussionmentioning

confidence: 99%

A transcriptomic analysis of skeletal muscle tissues reveals promising candidate genes and pathways accountable for different daily weight gain in Hanwoo cattle

Sheet,

Jang,

Kim

et al. 2024

Sci Rep

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“…Du et al [ 2 ] mentioned that the Wnt signaling pathway could increase myogenesis and reduces adipogenesis in skeletal muscle, regulating body fat and reducing obesity susceptibility during fetus development. Additionally, Pan et al [ 48 ] reported a genome identification and described that the Wnt family genes in cattle affect adipocyte differentiation in muscle. Thus, adequate levels of nutrients during gestation will increase Wnt signaling encouraging more myogenesis in early and mid-gestation [ 2 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

“…Thus, adequate levels of nutrients during gestation will increase Wnt signaling encouraging more myogenesis in early and mid-gestation [ 2 , 49 ]. Therefore, at the end of gestation, adipogenesis increases, and myogenesis is inhibited due to a lack of signaling of Wnt [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of maternal calcium propionate supplementation on offspring productivity and meat metabolomic profile in sheep

Pérez Segura,

Ramirez,

Relling

et al. 2023

PLoS ONE

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This study determined the effect of dietary calcium propionate (CaPr) as a source of energy supplementation during the First Half of Gestation (FMG), the Second Half of Gestation (SMG), and during All Gestation (AG), on offspring post-weaning growth performance, meat quality, and meat metabolomic profile. Thirty-one pregnant ewes were assigned to one of four treatments: a) supplementation of 30 gd−1 of CaPr during the first half of gestation (day 1 to day 75, n = 8) (FMG); b) supplementation of 30 gd−1 of CaPr during the second half of gestation (day 76 to day 150, n = 8) (SMG); c) supplementation of 30 gd−1 of CaPr during all gestation (AG, n = 8); d) no CaPr supplementation (control; CS, n = 7). The ewes were ad libitum fed a basal diet based on oat hay and corn silage. Ewes were distributed in a completely randomized unbalanced design to four treatments. The FMG group had lower (P ≤ 0.05) birth weight and weaning weight than the CS group. However, the average daily gain was similar across all treatments. Empty body weight and FMG had lower values (P ≤ 0.05) than the other groups. Both FMG and AG had lower hot carcass weight (P ≤ 0.05) compared to CS, while CaPr treatments resulted in reduced hot carcass yield (P ≤ 0.05). Meat color and texture were similar among treatments. A principal component analysis between gestation stages showed a trend for separating CS and FMG from SMG and AG, and that was explained by 93.7% of the data variability (PC1 = 87.9% and PC2 = 5.8%). Regarding meat metabolomic profile, 23 compounds were positively correlated between all treatments. Only 2 were negatively correlated (eicosane and naphthalene 1,2,3); but tetradecanoic acid, hexadecane, undecane 5-methyl, (-)-alpha, hexadecenoic acid, octadecanoic acid, and octadecane had a highly significant correlation (P ≤ 0.05). Overall, dam supplementation with CaPr during different periods of gestation provoked changes in meat metabolites related to the biosynthesis of fatty acids in lambs without negative changes in lamb’s growth performance and carcass quality.

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“…Among them, novel346_mature, novel295_mature, novel206_mature, novel355_mature et.al were involved in the Wnt signal pathway through banding with WNT2B, RSPO3, WNT5A, FOSL1 et.al. Wnt family genes are specifically expressed in bovine adipocytes, in which WNT2B regulates fat differentiation by activating FZD5 ( 56 ). RSPO3 promotes osteogenic differentiation of adipose stem cells through the LGR4-ERK signaling pathway ( 57 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of potential miRNA-mRNA regulatory network and the key miRNAs in intramuscular and subcutaneous adipose

Feng

Liu²,

Yousuf³

et al. 2022

Front. Vet. Sci.

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Intramuscular fat (IMF) is an important indicator for evaluating meat quality. Breeds with high IMF content are often accompanied by high subcutaneous fat (SCF), severely affecting the meat rate of pigs. Studying the mechanisms of miRNAs related to lipogenesis and lipid metabolism has important implications for pig breeding. We constructed two small RNA libraries from intramuscular and subcutaneous fat to evaluate the patterns of lipogenesis in Laiwu pig, a Chinese breed. A total of 286 differentially expressed miRNAs (DEmiRNAs), including 193 known miRNA and 93 novel miRNAs, were identified from two types of adipose. GO and KEGG enrichment analysis for DEmiRNAs showed that their target genes involved in many adipogenesis and lipid metabolism biological processes and signaling pathways, such as Wnt signaling pathway,MAPK signaling pathway, Hippo signaling pathway, PI3K-Akt signaling pathway, Melanogenesis, Signaling pathways regulating pluripotency of stem cells and so on. Then, we constructed a miRNA-mRNA interaction network to find out which miRNAs were the key miRNAs of regulation in Wnt signaling pathway. In this pathway, miR-331-3p, miR-339-5p, miR-874 and novel346_mature target PPARD, WNT10B, RSPO3, WNT2B. This study provides a theoretical basis for further understanding the post-transcriptional regulation mechanism of meat quality formation and predicting and treating diseases caused by ectopic fat.

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Genome-wide identification and expression profiling analysis of Wnt family genes affecting adipocyte differentiation in cattle

Cited by 10 publications

References 64 publications

A transcriptomic analysis of skeletal muscle tissues reveals promising candidate genes and pathways accountable for different daily weight gain in Hanwoo cattle

A transcriptomic analysis of skeletal muscle tissues reveals promising candidate genes and pathways accountable for different daily weight gain in Hanwoo cattle

Effects of maternal calcium propionate supplementation on offspring productivity and meat metabolomic profile in sheep

Identification of potential miRNA-mRNA regulatory network and the key miRNAs in intramuscular and subcutaneous adipose

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