Genome-wide identification and functional characterization of CDPK gene family reveal their involvement in response to drought stress in Gossypium barbadense

Abstract

Background Calcium dependent protein kinases (CDPKs) are a class of important calcium signal sensing response proteins, which play an important regulatory role in response to abiotic stress. However, researchers have not been excavated CDPKs’ role in drought in sea-island cotton(Gossypium barbadense L. ‘H7124’). Results Eighty-four CDPK genes have been identified in G. barbadense. These GbCDPK genes are unevenly distributed on 26 chromosomes, and segmental duplication is the significant way for the extension of CDPK family. Also, members within the same subfamily share a similar gene structure and motif composition. There are a large number of cis-elements involved in plant growth and response to stresses in the promoter regions of GbCDPKs. Additionally, these GbCDPKs show differential expression patterns in cotton tissues. The transcription levels of most genes were markedly altered in cotton under heat, cold, salt and PEG treatments, while the expressions of some GbCDPKs were induced in cotton under drought stress. Among these drought-induced genes, we selected GbCDPK32, GbCDPK68, GbCDPK74, GbCDPK80 and GbCDPK83 for further functional characterization by virus-induced gene silencing (VIGS) method. Conclusions In conclusion, the principal findings of this prospective study are that CDPKs were associated with drought. These findings provide a solid foundation for the development of future molecular mechanism in sea-island cotton.

The CDPK Gene Family in Mustard (Brassica juncea L.): Genome-Wide Identification and Expression Analysis under Cold Stress

“…In Solanum habrochaites, 11 ShCDPK family members were found to have LTR element, and the expression of a set of ShCDPK genes changed to a certain extent under cold stress [39]. Similar results were also found in other plants, such as Gossypium barbadense [66] and Medicago truncatula [67]. Moreover, the expression of ten selected BjuCDPK genes was observably induced by cold stress based on qRT-PCR analysis (Figure 6B), further supporting the RNA-seq data.…”

Genome-wide identification of R-SNARE gene family in upland cotton and function analysis of GhVAMP72l response to drought stress

“…To investigate the roles of R-SNAREs in cotton drought resistance, GhVAMP72l was silenced using VIGS technology ( Shi and Zhu, 2022 ). The results showed that GhVAMP72l -silenced plants were more sensitive to drought stress than control plants ( Figures 6B, C ), indicating that the drought resistance of GhVAMP72l -silenced plants was significantly reduced.…”

Combined physiological and transcriptome analysis revealed the response mechanism of Pogostemon cablin roots to p-hydroxybenzoic acid

“…Studies have shown that CDPKs are involved in regulating the response of various plants to environmental stress. Various environmental factors, such as drought and salt injury, can cause the differential expression of calcium-dependent protein kinase genes and the specific accumulation of their mRNAs ( Li et al, 2019 ; Crizel et al, 2020 ; Shi and Zhu, 2022 ). Combined with the transcriptome data in this study, it was found that the Ca 2+ /calmodulin-dependent EF-Hand protein kinase and calcium-dependent protein kinase-related genes were highly expressed in the roots of patchouli under the allelopathic effects of p-HBA, positively responding to this stress.…”