Genome-Wide Identification, Characterization, and Expression Analysis Related to Low-Temperature Stress of the CmGLP Gene Family in Cucumis melo L.

Abstract

Germin-like protein (GLP) participates in plant growth and development and plays an important role in plant stress. In the present study, 22 CmGLPs belonging to five classes were identified in the melon genome. Each member of the CmGLPs family contains a typical Cupin_1 domain. We conducted a genome-wide analysis of the melon GLP gene family characterization. CmGLPs were randomly distributed in the melon chromosomes, with the largest number on chromosome 8, having eight family members. Gene duplication events drive the evolution and expansion of the melon GLP gene family. Based on the phylogenetic tree analysis of GLP proteins in melon, rice, Arabidopsis, and cucumber, it was found that the GLP gene families of different species have diverged in evolution. Based on qRT-PCR results, all members of the CmGLP gene family could be expressed in different tissues of melon. Most CmGLP genes were up-regulated after low-temperature stress. The relative expression of CmGLP2-5 increased by 157.13 times at 48 h after low-temperature treatment. This finding suggests that the CmGLP2-5 might play an important role in low-temperature stress in melon. Furthermore, quantitative dual LUC assays indicated that CmMYB23 and CmWRKY33 can bind the promoter fragment of the CmGLP2-5. These results were helpful in understanding the functional succession and evolution of the melon GLP gene family and further revealed the response of CmGLPs to low-temperature stress in melon.

Identification and characterization of the cupin_1 domain-containing proteins in ma bamboo (Dendrocalamus latiflorus) and their potential role in rhizome sprouting

“…CDP family includes GLP members that contain a single cupin_1 domain, which are related to hormone-mediated development processes ( Murphy and Peer, 2022 ). GLPs have been identified in multiple species ( Davidson et al., 2010 ; Cheng et al., 2014 ; Li et al., 2016 ; Zhang et al., 2022 ; Li et al., 2023 ), and whole CDP family members have also been identified in B. napus ( He et al., 2022 ). In our cognition, the distribution and functional diversity of CDP family in ma bamboo have not been studied.…”

“…The GLP proteins are derived from the tissue-specific posttranslational processing of the proglucagon peptide. For example, GLP proteins in Cucumis melo responded to low temperatures, and CmGLP2-5 may have a key role in this process ( Zhang et al., 2022 ). Overexpression of DIGLP1-5-1 in the globular embryos of Dimocarpus longan increased the accumulation of lignin and lowered the H 2 O 2 content by modulating the activities of ROS-related enzymes ( Li et al., 2023 ).…”

Genetic Diversity and Molecular Breeding of Melon (Cucumis melo L.): A review

“…Salinity, osmotic stress, MATE heavy metals 7,8 Low temperature 11 CmGLP2-5 Autotoxicity 33 MYB Saline alkali response 33 CmMYB2R40…”

“…A genomic study focused on characterizing CmGLP gene family in melon has been found that CmGLP2-5 might be directly associated with low-temperature stress in melon 11 (Table 2). These finding would be helpful in melon molecular breeding programmes for low-temperature stress tolerant cultivars.…”

“…Typically, the genome-wide association analysis and gene mapping studies are carried out by the following procedure (i) Extraction of the genomic DNA of an individual plant, (ii) Whole-genome resequencing (or) Bulked segregant analysis (BSA) sequencing using primers designed based on the known genome sequence to identify linked single nucleotide polymorphisms (SNPs) and polymorphic markers (QTLs), (iii) Construction of a genetic map, (iv) Functional annotation and sequence analysis of the candidate gene based on the known genome sequence, (v) Characterization of the novel gene and validation of molecular maker in a natural population. 1,[6][7][8][9][10][11][12] Whereas, the transcriptome analysis is performed by the following procedure. (a) Extraction of the total RNA of an individual plant, (b) cDNA libraries construction and resequencing, (c) Quantitative Reverse Transcription PCR (qRT-PCR) using the primers designed for candidate genes based on the known genome sequence, (d) Identification and annotation of differentially expressed genes.…”