Genome-wide identification, characterization and expression of HSP 20 gene family in dove

Abstract

Davidia involucrata is a significant living fossil with high abiotic stress tolerance. Although heat shock protein 20 (HSP20) has already been linked to heat stress, nothing is known about HSP20 family protein activities in D. involucrata. The functional dynamics of the D. involucrata HSP20 (DiHSP20) gene family were identified and characterized using a thorough genome-wide investigation. From the genome of D. involucrata, a total of 42 HSP20 genes were identified, which are distributed across 16 chromosomes. The DiHSP20 proteins were grouped into seven separate subfamilies by our phylogenetic analysis, which was validated by the conserved motif composition and gene structure studies. Segmental duplication events were shown to play a crucial role in the expansion of the DiHSP20 gene family. Synteny analysis revealed that 19 DiHSP20 genes of D. involucrata shared a syntenic connection with Arabidopsis genes, 39 with C. acuminata genes, and just 6 with O. sativa genes. Additionally, heat stress differently enhanced the expression levels of D. involucrata HSP20 genes. After 1 hour of heat treatment, the expression levels of most DiHSP20 genes, particularly DiHSP20-7, DiHSP20-29, DiHSP20-30, DiHSP20-32, and DiHSP20-34, were dramatically increased, suggestted that they might be employed as heat tolerance candidate genes. Overall, these findings add to our knowledge of the HSP20 family genes and provide helpful information for breeding heat stress resistance in D. involucrata.

Genome-wide analysis of the HSP20 gene family and its response to heat and drought stress in Coix (Coix lacryma-jobi L.)

“…2 , Table 2 ), indicating that tandem duplication and segmental duplication have promoted the expansion and evolution of the ClHSP20 gene family. Similar duplication patterns were also found in rice [ 7 ], maize [ 29 ], and dove tree [ 28 ]. Interestingly, all three tandem duplicated gene pairs ( ClHSP20-10/ClHSP20-11; ClHSP20-25/ClHSP20-26; ClHSP20-30/ClHSP20-31 ) and two segmentally duplicated gene pairs ( ClHSP20-14/ClHSP20-29; ClHSP20-22/ClHSP20-32 ) displayed similar expression patterns in different tissues (Figs.…”

“…These results are similar to the findings in previous reports for other plant species. However, many plant species lack some of the HSP20 subfamilies; for example, Arabidopsis lacks the VI and CVII subfamilies [ 21 ], Populus lacks the CVI subfamily [ 35 ], rice lacks the CIV and CVII subfamilies [ 7 ], dove tree lacks the CII and CIII subfamilies [ 28 ], and pepper lacks the CIV and CV subfamilies [ 20 ]. Similarly, the ER, CIV, and CX subfamilies are absent from the HSP20 gene family of Coix, indicating that gene loss and expansion are common phenomena during the evolution process.…”

“…Since the discovery of the importance of HSP20 genes in response to various biotic and abiotic stresses, the HSP20 family of genes has been identified and characterized in dozens of plant species, such as Arabidopsis ( Arabidopsis thaliana ) [ 21 ], rice ( Oryza sativa ) [ 7 ], soybean ( Glycine max ) [ 22 ], tomato ( Solanum lycopersicum ) [ 23 ], cotton ( Gossypium hirsutum ) [ 24 ], potato ( Solanum tuberosum ) [ 25 ], grape ( Vitis vinifera ) [ 26 ], barley ( Hordeum vulgare ) [ 27 ], dove tree ( Davidia involucrata ) [ 28 ], maize ( Zea mays ) [ 29 ], rowan ( Sorbus pohuashanensis ) [ 30 ], and pepper ( Capsicum annuum ) [ 31 ], using genome-wide functional analysis. However, the HSP20 genes have not been thoroughly identified in Coix.…”

Genome‐wide identification and expression analysis of heat shock protein gene family in cassava

“…Cis‐acting regulatory elements (CAREs) serve as crucial molecular switches for the transcriptional regulation of complex and dynamic gene networks (Yamaguchi‐Shinozaki & Shinozaki, 2005). In the present study, we discovered a significant presence of stress‐ and hormone‐responsive elements; these types of cis‐acting elements were also found in both dove ( Davidia involucrate ) and barley ( Hordeum vulgar L.) (Chaudhary et al., 2019; Hou et al., 2022). These CAREs within Hsp s suggest that Hsp s regulate various physiological processes and plant growth in response to various stimuli.…”