Genome-Wide Identification, Characterization and Expression Profiling of myosin Family Genes in Sebastes schlegelii

Abstract

Myosins are important eukaryotic motor proteins that bind actin and utilize the energy of ATP hydrolysis to perform a broad range of functions such as muscle contraction, cell migration, cytokinesis, and intracellular trafficking. However, the characterization and function of myosin is poorly studied in teleost fish. In this study, we identified 60 myosin family genes in a marine teleost, black rockfish (Sebastes schlegelii), and further characterized their expression patterns. myosin showed divergent expression patterns in adult tissues, indicating they are involved in different types and compositions of muscle fibers. Among 12 subfamilies, S. schlegelii myo2 subfamily was significantly expanded, which was driven by tandem duplication events. The up-regulation of five representative genes of myo2 in the skeletal muscle during fast-growth stages of juvenile and adult S. schlegelii revealed their active role in skeletal muscle fiber synthesis. Moreover, the expression regulation of myosin during the process of myoblast differentiation in vitro suggested that they contribute to skeletal muscle growth by involvement of both myoblast proliferation and differentiation. Taken together, our work characterized myosin genes systemically and demonstrated their diverse functions in a marine teleost species. This lays foundation for the further studies of muscle growth regulation and molecular mechanisms of indeterminate skeletal muscle growth of large teleost fishes.

Identification, Characterization and Functional Analysis of Fibroblast Growth Factors in Black Rockfish (Sebastes schlegelii)

“…Here, S. schlegelii FGF genes showing a response to myoblast differentiation were selected to investigate the potential functions of FGF genes in muscle growth in vivo. Our current results show that all selected FGF genes were dynamically expressed during the muscle development of juvenile S.schlegelii (from 20 dpp to 90 dpp) ( Figure 4 B), among which, the expression levels of FGF10a , FGF19 , FGF6a , and FGF7 were found to significantly increase from 50 dpp to 75 dpp, corresponding to the periods during which the number and size of muscle fibers significantly increased [ 33 ], indicating their vital roles in contributing to the muscle growth.…”

“…The eif5a1 gene was used as the reference gene for standardizing the expressions of genes under testing [ 68 ]. The qRT-PCR analysis was performed under conditions as described previously [ 33 ]. The relative expressions of tested genes were calculated using the 2 −ΔΔCt comparative Ct method.…”

“…High-quality chromosome-level genome sequencing provides a useful tool to perform comprehensive gene analysis [ 31 ]. In our previous studies, we have demonstrated that pax3 / pax7 could be reliable markers for muscle satellite cells and function in the repair of muscle, as well as myosin genes, participating in muscle development via promoting myoblast proliferation or differentiation [ 32 , 33 ]. Here, we identified a total of 24 FGF genes in S. schlegelii and analyzed their dynamic expressions in various tissues of S. schledelli at different developmental stages.…”

Identification of Candidate Genes and Functional Pathways Associated with Body Size Traits in Chinese Holstein Cattle Based on GWAS Analysis

“…In our study, this SNP was located within the MYH15 gene, which belongs to the myosin heavy chain gene family. Myosins are superfamily genes of eukaryotic motor proteins that bind actin and use ATP hydrolysis energy to contribute significantly to a wide range of biological processes, such as muscle contraction, cytokinesis, cell motility and contractility, and intracellular trafficking [ 33 ]. A previous meta-analysis of GWAS revealed that the MYH15 gene is listed as a plausible candidate gene for detecting pleiotropic polymorphisms for body height, reproduction, and fatness in beef cattle [ 34 ], as well as pleiotropic effects on body composition in sheep [ 35 ].…”

Dietary antarctic krill improves antioxidant capacity, immunity and reduces lipid accumulation, insights from physiological and transcriptomic analysis of Plectropomus leopardus

“…Total RNA extraction of intestines and livers was performed by TRIzol reagent (Invitrogen, Carlsbad, CA, United States) according to the protocols of our lab [ 40 ]. After removing genomic DNA by Dnase I (TaKaRa, Dalian, China), the quality and quantity of RNA were evaluated by 1.5% agarose gel electrophoresis and NanoPhotometer Pearl (Implen GmbH, Munich, Germany), respectively.…”