Genome-wide Identification, Classification, Evolutionary Expansion and Expression of Rboh Family Genes in Pepper (Capsicum annuum L.)

Zhang, Jianwei; Xie, Yongdong; Ali, Barkat; Ahmed, Waleed; Tang, Yi; Li, Huanxiu

doi:10.1007/s12042-021-09286-3

Cited by 22 publications

(32 citation statements)

References 45 publications

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“…Prediction programs for subcellular localization showed that the seven CsRboh proteins were localized in the cell membrane. The results are in agreement with those obtained in other studies [ 10 , 13 , 14 , 16 , 28 ], suggesting Rbohs’ potential role in the regulation of ROS generation. Notably, few Rboh proteins were predicted to localize in chloroplast thylakoid membranes or mitochondria.…”

Section: Discussionsupporting

confidence: 93%

“…Here, we identified a total of seven Rboh genes from the Citrus sinensis genome ( Table 1 ). The number of Rboh genes in Citrus is similar to previous reports of grapes [ 8 ], strawberries [ 12 ], jatropha [ 13 ], cassava [ 14 ], and pepper [ 16 ], but relatively small. Prediction programs for subcellular localization showed that the seven CsRboh proteins were localized in the cell membrane.…”

Section: Discussionsupporting

confidence: 87%

“…Rbohs are encoded by multiple genes, and the gene family has been identified and characterized in some plant species. So far, ten Rboh genes were found in Arabidopsis [ 6 ], nine in rice [ 7 ], seven in grapes [ 8 ], fourteen in Nicotiana tabacum [ 9 ], twenty-six in upland cotton [ 10 ], nine in apples [ 11 ], seven in strawberries [ 12 ], seven in jatropha [ 13 ], eight in cassava [ 14 ], fourteen in Brassica rapa [ 15 ], and eight in pepper [ 16 ]. Analysis of gene expression patterns may help to understand the possible function of Rboh family genes.…”

Section: Introductionmentioning

confidence: 99%

“…In upland cotton, Ghrboh15 and Ghrboh16 were expressed constitutively in the stamen [ 10 ]. In pepper, CaRbohA , CaRbohB , and CaRbohD genes were highly expressed in the five tissues, but CaRbohC , CaRbohF , and CaRbohG genes were weakly expressed [ 16 ]. This differential tissue expression of Rboh genes has also been widely described in other plants [ 9 , 11 , 12 , 18 ], implying that the Rboh genes played important and diverse functional roles in different tissues.…”

Section: Introductionmentioning

confidence: 99%

“…The expression of seven NtabRboh genes ( A , B , C , M , N , O , and P ) were increased markedly following cold treatment in Nicotiana tabacum leaves [ 9 ]. In pepper, cold treatment significantly enhanced the expression of CaRbohA and CaRbohB genes [ 16 ]. These results indicate that Rboh genes are involved in cold stress responses, and the roles need to be further confirmed by transgenic studies in the future.…”

Section: Introductionmentioning

confidence: 99%

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Genome Wide Identification of Respiratory Burst Oxidase Homolog (Rboh) Genes in Citrus sinensis and Functional Analysis of CsRbohD in Cold Tolerance

Zhang

Luo

et al. 2022

IJMS

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Respiratory burst oxidase homologs (Rbohs) are critical enzymes involved in the generation of reactive oxygen species (ROS) that play an important role in plant growth and development as well as various biotic and abiotic stresses in plants. Thus far, there have been few reports on the characterization of the Rboh gene family in Citrus. In this study, seven Rboh genes (CsRbohA~CsRbohG) were identified in the Citrus sinensis genome. The CsRboh proteins were predicted to localize to the cell membrane. Most CsRbohs contained four conserved domains, an EF-hand domain, and a transmembrane region. Phylogenetic analysis demonstrated that the CsRbohs were divided into five groups, suggesting potential distinct functions and evolution. The expression profiles revealed that these seven CsRboh genes displayed tissue-specific expression patterns, and five CsRboh genes were responsive to cold stress. Fourteen putative cis-acting elements related to stress response, hormone response, and development regulation were present within the promoters of CsRboh genes. The in-silico microRNA target transcript analyses indicated that CsRbohE might be targeted by csi-miR164. Further functional and physiological analyses showed that the knockdown of CsRbohD in trifoliate orange impaired resistance to cold stress. As a whole, our results provide valuable information for further functional studies of the CsRboh genes in response to cold stress.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Genome Wide Identification of Respiratory Burst Oxidase Homolog (Rboh) Genes in Citrus sinensis and Functional Analysis of CsRbohD in Cold Tolerance

Zhang

Luo

et al. 2022

IJMS

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Cloning and expression analysis of Aerenchyma development related ShRBOH1 gene in Saussurea inversa Raab-Straube

Dai,

Shi,

et al. 2024

Braz. J. Bot

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Saussurea inversa Raab-Straube is a typical alpine plant with well-developed aerenchyma as its most representative structural feature. It has important theoretical significance for investigating the adaptive mechanisms of alpine plants to the environment through studying the formation and development of aerenchyma in S. inversa. In this study, the aerenchyma development-related gene ShRBOH1 (GenBank accession number OP727799) was cloned from S. inversa by RT-PCR with RACE technology. Sequence analysis showed that the cDNA of ShRBOH1 was 2,158 bp in length, which contained an open reading frame of 2,031 bp encoding 676 amino acids. Subcellular localization prediction showed that the ShRBOH1 protein was located in the cytoplasmic membrane, which was consistent with the localization of the NADPH protein. Comparison of homology amino acid sequence indicated that ShRBOH1 had higher similarity with CcRBOH1 of Cynara cardunculus L.. The qRT-PCR analysis showed that the ShRBOH1 gene was expressed in roots, stems and leaves, and the expression level in stems was significantly lower than in other tissues; the expression of the ShRBOH1 gene was down-regulated under UV stress, up-regulated under cold stress, and first up-regulated, then down-regulated and finally up-regulated under hypoxia stress. By comparing the changes in reactive oxygen species (ROS) content under the three kinds of stresses, the results showed that the changes were significant only under hypoxic stress and were positively correlated with the expression of ShRBOH1. It is hypothesized that ShRBOH1 positively regulates the formation of aerenchyma by ROS mediation, suggesting that the aerenchyma is mainly an important structure for adapting to hypoxia stress in alpine environments.

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Low R:FR light ratio enhances calcium nitrate resistance and stomatal movement in tomato seedlings by regulating H2O2 accumulation

Zhou,

Ye,

Tang

et al. 2023

Horticultural Plant Journal

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Genome-wide Identification, Classification, Evolutionary Expansion and Expression of Rboh Family Genes in Pepper (Capsicum annuum L.)

Cited by 22 publications

References 45 publications

Genome Wide Identification of Respiratory Burst Oxidase Homolog (Rboh) Genes in Citrus sinensis and Functional Analysis of CsRbohD in Cold Tolerance

Genome Wide Identification of Respiratory Burst Oxidase Homolog (Rboh) Genes in Citrus sinensis and Functional Analysis of CsRbohD in Cold Tolerance

Cloning and expression analysis of Aerenchyma development related ShRBOH1 gene in Saussurea inversa Raab-Straube

Low R:FR light ratio enhances calcium nitrate resistance and stomatal movement in tomato seedlings by regulating H2O2 accumulation

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