Genome-wide identification of soybean Shaker K+ channel gene family and functional characterization of GmAKT1 in transgenic Arabidopsis thaliana under salt and drought stress

Chen, Feng; He, Chengming; Wang, Yifan; Xu, Hehan; Xu, Keheng; Zhao, Yu; Yao, Bowen; Zhang, Yinhe; Zhao, Yan; Carther, Kue Foka Idrice; Luo, Jun; Sun, Daqian; Gao, Hongtao; Wang, Fawei; Li, Xiaowei; Liu, Weican; Dong, Yanjun; Wang, Nan; Zhou, Yonggang; Li, Haiyan

doi:10.1016/j.jplph.2021.153529

Cited by 22 publications

(19 citation statements)

References 54 publications

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“…Considering the sensitive response of Group III to hormone signals, we randomly selected CsWRKY18 for further functional analysis. We generated transgenic A. thaliana plants overexpressing the CsWRKY18 gene under the CaMV 35S promoter, according to the general method ( Clough and Bent, 1998 ; Feng et al, 2021 ) and we established 23 homozygous transgenic lines using hygromycin selection and PCR. Three T2-independent lines, L2, L11, and L13, were chosen for further phenotypic analysis according to the PCR analysis ( Figure 7B ).…”

Section: Methodsmentioning

confidence: 99%

Genome-wide identification of Cymbidium sinense WRKY gene family and the importance of its Group III members in response to abiotic stress

Wei

Jin²,

Gao³

et al. 2022

Front. Plant Sci.

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Transcription factors (TFs) of the WRKY family play pivotal roles in defense responses and secondary metabolism of plants. Although WRKY TFs are well documented in numerous plant species, no study has performed a genome-wide investigation of the WRKY gene family in Cymbidium sinense. In the present work, we found 64 C. sinense WRKY (CsWRKY) TFs, and they were further divided into eight subgroups. Chromosomal distribution of CsWRKYs revealed that the majority of these genes were localized on 16 chromosomes, especially on Chromosome 2. Syntenic analysis implied that 13 (20.31%) genes were derived from segmental duplication events, and 17 orthologous gene pairs were identified between Arabidopsis thaliana WRKY (AtWRKY) and CsWRKY genes. Moreover, 55 of the 64 CsWRKYs were detectable in different plant tissues in response to exposure to plant hormones. Among them, Group III members were strongly induced in response to various hormone treatments, indicating their potential essential roles in hormone signaling. We subsequently analyzed the function of CsWRKY18 in Group III. The CsWRKY18 was localized in the nucleus. The constitutive expression of CsWRKY18 in Arabidopsis led to enhanced sensitivity to ABA-mediated seed germination and root growth and elevated plant tolerance to abiotic stress within the ABA-dependent pathway. Overall, our study represented the first genome-wide characterization and functional analysis of WRKY TFs in C. sinense, which could provide useful clues about the evolution and functional description of CsWRKY genes.

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Section: Methodsmentioning

confidence: 99%

Genome-wide identification of Cymbidium sinense WRKY gene family and the importance of its Group III members in response to abiotic stress

Wei

Jin²,

Gao³

et al. 2022

Front. Plant Sci.

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“…The K + channels are divided into three families, including Shaker, TPK, and Kir-like K + channel families ( Lebaudy et al, 2007 ). Among them, the Shaker K + channel is the most extensively studied gene family and has been identified in many plant species, including Arabidopsis ( Dreyer and Uozumi, 2011 ), rice ( Amrutha et al, 2007 ; Hwang et al, 2013 ), maize ( Bauer et al, 2000 ), poplar ( Zhang et al, 2010 ), sweet potato ( Jin et al, 2021 ), and soybean ( Feng et al, 2021 ). Shaker K + channels are involved in K + uptake from soil and transport among different tissues.…”

Section: Introductionmentioning

confidence: 99%

“…ZMK1, a homolog of AKT1 in Zea May , mediates K + uptake in coleoptile cells and its expression is induced by auxin ( Philippar et al, 1999 , 2006 ). Over-expressing GmAKT1 of soybean in Arabidopsis enhances root length and K + concentration under drought and salt stress, suggesting that GmAKT1 plays a role in soybean response to such stresses ( Feng et al, 2021 ). The GORK channel associated K + efflux and the channel’s sub-cellular location in the guard cell is regulated by ABA, a drought-related phytohormone, through the OPEN STOMATA 1 (OST1) kinase involved signaling system ( Chen et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of Shaker K+ Channel Gene Family in Foxtail Millet (Setaria italica) and Their Role in Stress Response

et al. 2022

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Potassium (K+) is one of the indispensable elements in plant growth and development. The Shaker K+ channel protein family is involved in plant K+ uptake and distribution. Foxtail millet (Setaria italica), as an important crop, has strong tolerance and adaptability to abiotic stresses. However, no systematic study focused on the Shaker K+ channel family in foxtail millet. Here, ten Shaker K+ channel genes in foxtail millet were identified and divided into five groups through phylogenetic analysis. Gene structures, chromosome locations, cis-acting regulatory elements in promoter, and post-translation modification sites of Shaker K+ channels were analyzed. In silico analysis of transcript level demonstrated that the expression of Shaker K+ channel genes was tissue or developmental stage specific. The transcription levels of Shaker K+ channel genes in foxtail millet under different abiotic stresses (cold, heat, NaCl, and PEG) and phytohormones (6-BA, BR, MJ, IAA, NAA, GA3, SA, and ABA) treatments at 0, 12, and 24 h were detected by qRT-PCR. The results showed that SiAKT1, SiKAT3, SiGORK, and SiSKOR were worth further research due to their significant responses after most treatments. The yeast complementation assay verified the inward K+ transport activities of detectable Shaker K+ channels. Finally, we found interactions between SiKAT2 and SiSNARE proteins. Compared to research in Arabidopsis, our results showed a difference in SYP121 related Shaker K+ channel regulation mechanism in foxtail millet. Our results indicate that Shaker K+ channels play important roles in foxtail millet and provide theoretical support for further exploring the K+ absorption mechanism of foxtail millet under abiotic stress.

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“…The K + channels are divided into three families, including Shaker, TPK, and Kirlike K + channel families (Lebaudy et al, 2007). Among them, the Shaker K + channel is the most extensively studied gene family and has been identified in many plant species, including Arabidopsis (Dreyer and Uozumi, 2011), rice (Amrutha et al, 2007;Hwang et al, 2013), maize (Bauer et al, 2000), poplar (Zhang et al, 2010), sweet potato (Jin et al, 2021), and soybean (Feng et al, 2021). Shaker K + channels are involved in K + uptake from soil and transport among different tissues.…”

Section: Introductionmentioning

confidence: 99%

“…ZMK1, a homolog of AKT1 in Zea May, mediates K + uptake in coleoptile cells and its expression is induced by auxin (Philippar et al, 1999(Philippar et al, , 2006. Over-expressing GmAKT1 of soybean in Arabidopsis enhances root length and K + concentration under drought and salt stress, suggesting that GmAKT1 plays a role in soybean response to such stresses (Feng et al, 2021). The GORK channel associated K + efflux and the channel's sub-cellular location in the guard cell is regulated by ABA, a drought-related phytohormone, through the OPEN STOMATA 1 (OST1) kinase involved signaling system (Chen et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide identification of soybean Shaker K+ channel gene family and functional characterization of GmAKT1 in transgenic Arabidopsis thaliana under salt and drought stress

Cited by 22 publications

References 54 publications

Genome-wide identification of Cymbidium sinense WRKY gene family and the importance of its Group III members in response to abiotic stress

Genome-wide identification of Cymbidium sinense WRKY gene family and the importance of its Group III members in response to abiotic stress

Identification and Characterization of Shaker K+ Channel Gene Family in Foxtail Millet (Setaria italica) and Their Role in Stress Response

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