Genome-wide long non-coding RNAs identified a panel of novel plasma biomarkers for gastric cancer diagnosis

Zheng, Rui; Liang, Jiayuan; Lü, Jiafeng; Li, Shuwei; Zhang, Gang; Wang, Xiaowei; Liu, Mengting; Wang, Weizhi; Chu, Haiyan; Tao, Guoquan; Zhao, Qinghong; Wang, Meilin; Du, Mulong; Fu, Qiang; Zhang, Zhengdong

doi:10.1007/s10120-018-00915-7

Cited by 31 publications

(20 citation statements)

References 36 publications

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“…9 Additionally, different plasma lncRNAs panels can be used as biomarkers for GC detection. 10,11 These data indicate that plasma lncRNAs may serve as biomarkers for GC.…”

Section: Introductionmentioning

confidence: 84%

<p>Plasma Exosomal Long Noncoding RNA lnc-SLC2A12-10:1 as a Novel Diagnostic Biomarker for Gastric Cancer</p>

Zheng

Zhang

Gao

et al. 2020

OTT

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Exosomes participate in cellular communications by transmitting active molecules, including long noncoding RNAs (lncRNAs) and are regarded as suitable candidates for disease diagnosis. This study aimed to identify gastric cancer (GC)-specific exosomal lncRNA and investigate the potential diagnostic value of plasma exosomal lncRNA in GC. Patients and Methods: Exosomes from the culture media (CM) of four GC cells (GCCs) and human gastric epithelial cells were isolated. Exosomal RNA was extracted, and lncRNA microarray assay was performed to identify GC-specific exosomal lncRNAs. The expression levels of the candidate exosomal lncRNAs were validated in 120 subjects via quantitative reverse transcription PCR (qRT-PCR). The receiver operating characteristic (ROC) curve and area under curve were used to estimate the diagnostic capacity. We investigated the potential relationship between plasma exosomal lncRNA expression and the clinicopathological parameters of GC. Results: A total of 199 exosomal lncRNAs were expressed at considerable higher levels in GCCs than those in normal controls, among which the top 10 upregulated lncRNAs were selected for further validation in cell, CM, and plasma. qRT-PCR revealed that lnc-SLC2A12-10:1 was remarkably upregulated in exosomes derived from patients with GC and GCCs. The area under the ROC curve was 0.776, which was higher than the diagnostic accuracies of CEA, CA 19-9, and CA72-4. The expression level of exosomal lnc-SLC2A12-10:1 was also significantly correlated with tumor size, TNM stage, lymph node metastasis, and degree of differentiation. The postoperative expression levels of exosomal lnc-SLC2A12-10:1 were lower compared with those of preoperative levels. Conclusion: Our study suggested that exosomal lnc-SLC2A12-10:1 may be a potential noninvasive biomarker for the diagnosis and prognosis monitoring of GC. Further large-scale studies are necessary to validate its performance in GC progression.

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“…9 Additionally, different plasma lncRNAs panels can be used as biomarkers for GC detection. 10,11 These data indicate that plasma lncRNAs may serve as biomarkers for GC.…”

Section: Introductionmentioning

confidence: 84%

<p>Plasma Exosomal Long Noncoding RNA lnc-SLC2A12-10:1 as a Novel Diagnostic Biomarker for Gastric Cancer</p>

Zheng

Zhang

Gao

et al. 2020

OTT

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“…These results suggest that CVB-D may suppress EMT by regulating the expression of Snail. RNA-seq techniques have been used for the study of numerous diseases, such as bladder, gastric, liver, cervical, colon and breast cancers (45)(46)(47)(48)(49)(50). RNA-seq is an effective tool for sequencing transcripts using high-throughput sequencing technology.…”

Section: Discussionmentioning

confidence: 99%

Cyclovirobuxine D inhibits colorectal cancer tumorigenesis via the CTHRC1‑AKT/ERK‑Snail signaling pathway

et al. 2020

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Cyclovirobuxine D (CVB-D) is an alkaloid, which is mainly derived from Buxus microphylla. It has been reported that CVB-D has positive effects on breast cancer, gastric cancer and other malignant tumors. However, to the best of our knowledge, there are no reports regarding the effects of CVB-D on colorectal cancer (CRC). The purpose of the present study was to determine the anticancer effects of CVB-D and further elucidate its molecular mechanism(s). DLD-1 and LoVo cell lines were selected to evaluate the antitumor effect of CVB-D. Cytotoxicity, viability and proliferation were evaluated by the MTT and colony formation assays. Flow cytometry was used to detect the effects on apoptosis and the cell cycle in CVB-D-treated CRC cells. The migration and invasion abilities of CRC cells were examined by wound healing and Transwell assays. In addition, RNA sequencing, bioinformatics analysis and western blotting were performed to investigate the target of drug action and clarify the molecular mechanisms. A xenograft model was established using nude mice, and ultrasound was employed to assess the preclinical therapeutic effects of CVB-D in vivo. It was identified that CVB-D inhibited the proliferation, migration, stemness, angiogenesis and epithelial-mesenchymal transition of CRC cells, and induced apoptosis and S-phase arrest. In addition, CVB-D significantly inhibited the growth of xenografts. It is notable that CVB-D exerted anticancer effects in CRC cells partly by targeting collagen triple helix repeat containing 1 (CTHRC1), which may be upstream of the AKT and ERK pathways. CVB-D exerted anticancer effects through the CTHRC1-AKT/ERK-Snail signaling pathway. Targeted therapy combining CTHRC1 with CVB-D may offer a promising novel therapeutic approach for CRC treatment.

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“…GUSBP11 and LINC00525 were found to participate in the development of several cancers. GUSBP11 overexpression accelerated the viability and invasion of gastric cancer cells (Zheng et al, 2019). Moreover, differentially expressed GUSBP11 was identified in head and neck squamous cell carcinoma (HNSCC) (Zheng et al, 2019).…”

Section: Immune-related Lncrnas Regulate the Immune Response In Periomentioning

confidence: 99%

“…GUSBP11 overexpression accelerated the viability and invasion of gastric cancer cells (Zheng et al, 2019). Moreover, differentially expressed GUSBP11 was identified in head and neck squamous cell carcinoma (HNSCC) (Zheng et al, 2019). LINC00525 enhanced the stemness and chemoresistance of colorectal cancer through the miR-507/ELK3 axis (Wang et al, 2019).…”

Section: Immune-related Lncrnas Regulate the Immune Response In Periomentioning

confidence: 99%

Expression Profiling and Cell Type Classification Analysis in Periodontitis Reveal Dysregulation of Multiple lncRNAs in Plasma Cells

Zhou

Cao

et al. 2020

Front. Genet.

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Objective: Periodontitis is a chronic inflammatory disease with a downregulated immune response. The mechanisms of the immune response, especially regarding immune-related long non-coding RNAs (lncRNAs), in periodontitis remain unclear. This study aimed to analyze the immune cell landscapes and immune-related transcriptome expression in periodontitis. Materials and Methods: The periodontitis-related microarray data set GSE16134 was downloaded from the Gene Expression Omnibus database. Then, the proportions of the infiltrated immune cell subpopulations were evaluated by Cell-type Identification By Estimating Relative Subsets Of RNA Transcripts (CIBERSORT). Differentially expressed immune-related genes (DEMGs) and lncRNAs were analyzed by the "limma" package in R software. Co-expression of DEMGs and lncRNAs in immune cell subpopulations was evaluated. Gene set enrichment analysis (GSEA) was performed to identify alterations in immune function through potential pathways. Results: Increased numbers of plasma cells were observed in periodontitis-affected tissues versus those of healthy tissues, while T cells were downregulated. A total of 51 DEMGs were identified, and 12 immune-related signaling pathways were enriched by GSEA, most of which were related to the stimulation and function of B cells and T cells. Only 3 differentially upregulated lncRNAs (FAM30A, GUSBP11, and LINC00525) were screened for the regulation of the immune response. Besides, the level of lncRNAs (FAM30A, GUSBP11, and LINC00525) expression were positively correlated with the fraction of plasma cells in periodontitis. Conclusion: The discovery of differentially expressed immune-related transcriptomes in periodontitis lesions helps to explain the regulation of the immune mechanism in the development of periodontitis.

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Genome-wide long non-coding RNAs identified a panel of novel plasma biomarkers for gastric cancer diagnosis

Cited by 31 publications

References 36 publications

<p>Plasma Exosomal Long Noncoding RNA lnc-SLC2A12-10:1 as a Novel Diagnostic Biomarker for Gastric Cancer</p>

<p>Plasma Exosomal Long Noncoding RNA lnc-SLC2A12-10:1 as a Novel Diagnostic Biomarker for Gastric Cancer</p>

Cyclovirobuxine D inhibits colorectal cancer tumorigenesis via the CTHRC1‑AKT/ERK‑Snail signaling pathway

Expression Profiling and Cell Type Classification Analysis in Periodontitis Reveal Dysregulation of Multiple lncRNAs in Plasma Cells

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