Genome-wide transcriptome analysis and characterization of the cytochrome P450 flavonoid biosynthesis genes in pigeon pea (Cajanus cajan)

Yang, Jie; Li, Hongquan; Ma, Ronghua; Chang, Yuan‐Hang; Qin, Xiangyu; Xu, Jian; Fu, Yujie

doi:10.1007/s00425-022-03896-1

Cited by 11 publications

(8 citation statements)

References 113 publications

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“…Treatments with ELICE16INDURES® also had a positive effect on increasing the level of the CYP enzyme, thereby catalyzing the plant's phytoalexin production too [ 65 ]. Gene expression changes of the CYP enzyme family and their effects were also investigated in soybean [ 25 ].…”

Section: Resultsmentioning

confidence: 99%

Natural immunity stimulation using ELICE16INDURES® plant conditioner in field culture of soybean

Decsi

Kutasy

Hegedűs

et al. 2023

Heliyon

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Section: Resultsmentioning

confidence: 99%

Natural immunity stimulation using ELICE16INDURES® plant conditioner in field culture of soybean

Decsi

Kutasy

Hegedűs

et al. 2023

Heliyon

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“…Insect tolerance [55] CYP79 Cyanogenic glucosides biosynthesis [56] Catharanthus roseus CYP72A1 Alkaloid biosynthesis Disease resistance [57] Ocimum basilicum CYP82D Biosynthesis of different secondary metabolites [58] Vitis vinifera CYP75 Flavonoid biosynthesis [59] Gossypium arboreum CYP706B1 Alteration of the isoprenoid pathway [60] Symphytum tuberosum CYP86A33 Biosynthesis of suberin [36] Pogostemon cablin CYP71, CYP77, CYP81, CYP82 Biosynthesis of sesquiterpenes [61] Citrus sp. UV-B-induced CYP gene Biosynthesis of 3 -hydroxylated flavonoids [62] Cajanus cajan CYP75B165 Biosynthesis of flavonoids [63] Glycine max CYP82D26 Isoflavonoid pathway biosynthesis [64] Legumes CYP93C Synthesis of legume specific isoflavonoid [65] Land plants CYP93B, CYP93E, CYP93G Saponin biosynthesis [66] Higher plants CYP74A Synthesis of allene oxide Biotic stress resistance [67]…”

Section: Biosynthesis Of the Cyanogenic Glucosidementioning

confidence: 99%

Cytochrome P450 Gene Families: Role in Plant Secondary Metabolites Production and Plant Defense

Chakraborty

Biswas

Dey

et al. 2023

JoX

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Cytochrome P450s (CYPs) are the most prominent family of enzymes involved in NADPH- and O2-dependent hydroxylation processes throughout all spheres of life. CYPs are crucial for the detoxification of xenobiotics in plants, insects, and other organisms. In addition to performing this function, CYPs serve as flexible catalysts and are essential for producing secondary metabolites, antioxidants, and phytohormones in higher plants. Numerous biotic and abiotic stresses frequently affect the growth and development of plants. They cause a dramatic decrease in crop yield and a deterioration in crop quality. Plants protect themselves against these stresses through different mechanisms, which are accomplished by the active participation of CYPs in several biosynthetic and detoxifying pathways. There are immense potentialities for using CYPs as a candidate for developing agricultural crop species resistant to biotic and abiotic stressors. This review provides an overview of the plant CYP families and their functions to plant secondary metabolite production and defense against different biotic and abiotic stresses.

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“…Qumoroyl quinate monooxygenase (CYP98A14) is a cytochrome P450 monooxygenase that catalyzes the hydroxylation of quinate to produce caffeoylquinate, a precursor Frontiers in Genetics frontiersin.org of chlorogenic acid and other phenolic compounds. In flavonoid biosynthesis, CYP98A14 is involved in the hydroxylation of p-coumaroylshikimate to form caffeoylshikimate, which is a key intermediate in the biosynthesis of a range of flavonoids, including anthocyanins, flavonols, and proanthocyanidins (Yang et al, 2022). This indicated that these three genes have significantly impacted the flavonoid biosynthesis pathway.…”

Section: Organismal Systemmentioning

confidence: 99%

De novo sequencing, assembly, and characterization of Asparagus racemosus transcriptome and analysis of expression profile of genes involved in the flavonoid biosynthesis pathway

Malik,

Dwivedi,

Rabuma

et al. 2023

Front. Genet.

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Asparagus racemosus is known for its diverse content of secondary metabolites, i.e., saponins, alkaloids, and a wide range of flavonoids. Flavonoids, including phenols and polyphenols, have a significant role in plant physiology and are synthesized in several tissues. Despite the diverse role of flavonoids, genetic information is limited for flavonoid biosynthesis pathways in A. racemosus. The current study explores full-scale functional genomics information of A. racemosus by de novo transcriptome sequencing using Illumina paired-end sequencing technology to elucidate the genes involved in flavonoid biosynthesis pathways. The de novo assembly of high-quality paired-end reads resulted in ∼2.3 million high-quality reads with a pooled transcript of 45,647 comprising ∼76 Mb transcriptome with a mean length (bp) of 1,674 and N50 of 1,868bp. Furthermore, the coding sequence (CDS) prediction analysis from 45,647 pooled transcripts resulted in 45,444 CDS with a total length and mean length of 76,398,686 and 1,674, respectively. The Gene Ontology (GO) analysis resulted in a high number of CDSs assigned to 25,342 GO terms, which grouped the predicted CDS into three main domains, i.e., Biological Process (19,550), Molecular Function (19,873), and Cellular Component (14,577). The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database was used to categorize 6,353 CDS into 25 distinct biological pathway categories, in which the majority of mapped CDS were shown to be related to translation (645), followed by signal transduction (532), carbohydrate metabolism (524), folding, sorting, and degradation (522). Among these, only ∼64 and 14 CDSs were found to be involved in the phenylpropanoid and flavonoid biosynthesis pathways, respectively. Quantitative Real-time PCR was used to check the expression profile of fourteen potential flavonoid biosynthesis pathway genes. The qRT-PCR analysis result matches the transcriptome sequence data validating the Illumina sequence results. Moreover, a large number of genes associated with the flavonoids biosynthesis pathway were found to be upregulated under the induction of methyl jasmonate. The present-day study on transcriptome sequence data of A. racemosus can be utilized for characterizing genes involved in flavonoid biosynthesis pathways and for functional genomics analysis in A. racemosus using the reverse genetics approach (CRISPR/Cas9 technology).

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Genome-wide transcriptome analysis and characterization of the cytochrome P450 flavonoid biosynthesis genes in pigeon pea (Cajanus cajan)

Cited by 11 publications

References 113 publications

Natural immunity stimulation using ELICE16INDURES® plant conditioner in field culture of soybean

Natural immunity stimulation using ELICE16INDURES® plant conditioner in field culture of soybean

Cytochrome P450 Gene Families: Role in Plant Secondary Metabolites Production and Plant Defense

De novo sequencing, assembly, and characterization of Asparagus racemosus transcriptome and analysis of expression profile of genes involved in the flavonoid biosynthesis pathway

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