Genome-wide transcriptome analysis reveals that a pleiotropic antibiotic regulator, AfsS, modulates nutritional stress response in Streptomyces coelicolor A3(2)

Lian, Wei; Jayapal, Karthik P.; Charaniya, Salim; Mehra, Sarika; Glod, Frank; Kyung, Yun Seung; Sherman, David H.; Hu, Wei Shou

doi:10.1186/1471-2164-9-56

Cited by 54 publications

(56 citation statements)

References 49 publications

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“…AfsS may provide some speciesspecific beneficial modulation of AfsR function by virtue of its (probably mostly indirect) influences on the transcription of various stress response and stationary-phase genes (136). More than 117 genes were up-or downregulated in an afsS disruption mutant (136). Disruption of afsS also causes diminished actinorhodin production during phosphate starvation, an observation underpinned by the finding that AfsR and PhoP bind to overlapping sequences in the promoter region of afsS (82).…”

Section: The Afsk/afsr/afss System and A Possible Interface With Hyphmentioning

confidence: 88%

“…AfsS (also called AfsR2) has been studied as an apparent positive regulator of antibiotic production since its dis- covery (135). It has some homology with the conserved linker domain 3 of sigma factors but is unlikely to function as a sigma factor on its own (136). A suggestion that AfsS might act cooperatively with sigma factors of the ECF subclass, which lack domain 3 (136), seems unlikely given that ECF sigma factors are ubiquitous while AfsS-like proteins are found in only some (not all) streptomycetes.…”

Section: The Afsk/afsr/afss System and A Possible Interface With Hyphmentioning

confidence: 99%

“…Disruption of afsS also causes diminished actinorhodin production during phosphate starvation, an observation underpinned by the finding that AfsR and PhoP bind to overlapping sequences in the promoter region of afsS (82). Although AfsS is usually thought of as a regulator of antibiotic biosynthetic genes, transcriptome analysis showed that it also has major effects on nutritional starvation genes, which might possibly be responsible for the effects on antibiotic production (136).…”

Section: The Afsk/afsr/afss System and A Possible Interface With Hyphmentioning

confidence: 99%

“…When present, afsS is seldom as closely linked to afsR as in S. coelicolor (though quite often the two genes are within a few tens of genes of each other). AfsS may provide some speciesspecific beneficial modulation of AfsR function by virtue of its (probably mostly indirect) influences on the transcription of various stress response and stationary-phase genes (136). More than 117 genes were up-or downregulated in an afsS disruption mutant (136).…”

Section: The Afsk/afsr/afss System and A Possible Interface With Hyphmentioning

confidence: 99%

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Molecular Regulation of Antibiotic Biosynthesis in Streptomyces

Liu

Chater

Chandra

et al. 2013

Microbiol Mol Biol Rev

608

536

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SUMMARY Streptomycetes are the most abundant source of antibiotics. Typically, each species produces several antibiotics, with the profile being species specific. Streptomyces coelicolor , the model species, produces at least five different antibiotics. We review the regulation of antibiotic biosynthesis in S. coelicolor and other, nonmodel streptomycetes in the light of recent studies. The biosynthesis of each antibiotic is specified by a large gene cluster, usually including regulatory genes (cluster-situated regulators [CSRs]). These are the main point of connection with a plethora of generally conserved regulatory systems that monitor the organism's physiology, developmental state, population density, and environment to determine the onset and level of production of each antibiotic. Some CSRs may also be sensitive to the levels of different kinds of ligands, including products of the pathway itself, products of other antibiotic pathways in the same organism, and specialized regulatory small molecules such as gamma-butyrolactones. These interactions can result in self-reinforcing feed-forward circuitry and complex cross talk between pathways. The physiological signals and regulatory mechanisms may be of practical importance for the activation of the many cryptic secondary metabolic gene cluster pathways revealed by recent sequencing of numerous Streptomyces genomes.

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Section: The Afsk/afsr/afss System and A Possible Interface With Hyphmentioning

confidence: 88%

Section: The Afsk/afsr/afss System and A Possible Interface With Hyphmentioning

confidence: 99%

Section: The Afsk/afsr/afss System and A Possible Interface With Hyphmentioning

confidence: 99%

Section: The Afsk/afsr/afss System and A Possible Interface With Hyphmentioning

confidence: 99%

See 2 more Smart Citations

Molecular Regulation of Antibiotic Biosynthesis in Streptomyces

Liu

Chater

Chandra

et al. 2013

Microbiol Mol Biol Rev

608

536

View full text Add to dashboard Cite

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“…Examples of these regulators are AfsK, AfsR, and AfsS. These proteins have been shown to influence secondary metabolism and morphogenesis in several species of Streptomyces (8,17,29,30,40,42).…”

mentioning

confidence: 99%

Complex Transcriptional Control of the Antibiotic Regulator afsS in Streptomyces: PhoP and AfsR Are Overlapping, Competitive Activators

2011

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β‐Lactam Antibiotics

Testero

Fisher

Mobashery

2010

Burger's Medicinal Chemistry and Drug Discovery

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The β‐lactam classes of antibacterials are preeminent in the treatment of bacterial infection due to their unparalleled clinical efficacy and clinical safety. Following the discovery of the penicillins, successive β‐lactam drug discovery has added the cephalosporin, penem cephamycin, clavulanate, monobactam, nocardicin, and carbapenem subclasses. The driving force behind much of this era of discovery is the staggering ability of pathogenic bacteria to adapt previous generations of the β‐lactam by the acquisition and expression of resistance mechanisms. Although many factors contribute to β‐lactam resistance, alterations to the molecular targets of the β‐lactams (the penicillin binding proteins) and the use of enzymes (the β‐lactamases) capable of the hydrolytic deactivation of the β‐lactams are paramount. This review traces the historical development of β‐lactam drug discovery, with emphasis on the most recent progress in the medicinal chemistry, biochemistry, and microbiology of the β‐lactams leading to the discovery of new generation β‐lactam antibacterials effective against the Gram‐negative and ‐positive bacterial pathogens of current medical concern.

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Genome-wide transcriptome analysis reveals that a pleiotropic antibiotic regulator, AfsS, modulates nutritional stress response in Streptomyces coelicolor A3(2)

Cited by 54 publications

References 49 publications

Molecular Regulation of Antibiotic Biosynthesis in Streptomyces

Molecular Regulation of Antibiotic Biosynthesis in Streptomyces

Complex Transcriptional Control of the Antibiotic Regulator afsS in Streptomyces: PhoP and AfsR Are Overlapping, Competitive Activators

β‐Lactam Antibiotics

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