Genome-wide transcriptome profiling reveals the mechanism of the effects of uniconazole on root development in Glycine Max

Han, Yanhe; Gao, Yamei; Shi, Ying; Du, Jidao; Zheng, Dianfeng

doi:10.1007/s12374-017-0028-9

Cited by 17 publications

(13 citation statements)

References 66 publications

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“…Gibberellin 2-beta-dioxygenase ( GA2ox ) is involved in GA inactivation, which is associated with Glycine max development 37 , might play critical roles in cell division 38 . The GA2ox transcript level was substantially higher from 30 to 48 days of culture, which was when explant reinvigoration was observed (Fig.…”

Section: Resultsmentioning

confidence: 99%

Reinvigoration of diploid strawberry (Fragaria vesca) during adventitious shoot regeneration

Wang

Yang

et al. 2019

Sci Rep

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Diploid strawberry (Fragaria vesca ‘Baiguo’) is a model plant for studying functional genomics in Rosaceae. Adventitious shoot regeneration is essential for functional genomics by Agrobacterium tumefaciens-mediated transformation. An efficient shoot regeneration method using diploid strawberry leaf explants was conducted on 1/2MS + 1/2B5 medium that contained 2.0 mg L−1 TDZ over 14 days of dark culture; this induced the maximum percentage of shoot regeneration (96.44 ± 1.60%) and the highest number of shoots per explant (23.46 ± 2.14) after 11 weeks of culture. The explants considerably enlarged after 12 days; then, turned greenish brown after 30 days, yellowish brown after 36 days, and completely brown and necrotic after 48 days. Large numbers of adventitious shoots were produced from 48 to 66 days, and the shoots elongated from 66 to 78 days; this represents a critical period of reinvigoration, which included 30 days for leaf explant chlorosis, 36 days for adventitious shoot appearance, and 48 days for generation of numerous shoots. During the reinvigoration process, higher expressions of the hormone synthesis-related genes Ciszog1, CKX2, CKX3, CKX7, YUC2, YUC6, YUC10, YUC9, and GA2ox were detected from 30 to 48 days. Our results indicate that these genes may regulate reinvigoration of shoot regeneration.

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Section: Resultsmentioning

confidence: 99%

Reinvigoration of diploid strawberry (Fragaria vesca) during adventitious shoot regeneration

Wang

Yang

et al. 2019

Sci Rep

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“…2). In our previous study, uniconazole treatment promoted root growth in soybean which indirectly indicated the inhibition effect of GA3 on root growth (Han et al 2017). The effects of exogenous GAs and GAs inhibitors on root development vary depending on the plant species and experimental conditions (Tanimoto 2012), so more studies in other species will provide further information.…”

Section: Discussionmentioning

confidence: 88%

“…Three biological replicates were used for all RNA-seq experiments from each sample. Further RNA purification, cDNA synthesis and sequencing were performed as previously described (Han et al 2017). Sequencing analysis was performed using a HiSeq 4000 platform (Illumina, San Diego, CA, USA) for paired end RNA sequencing (read length 2 × 150 bp).…”

Section: Phytohormone Analysis With Hplc-esi-msmentioning

confidence: 99%

Treatment of Glycine max seeds with gibberellins alters root morphology, anatomy, and transcriptional networks

Han¹,

Shi²,

Gao³

et al. 2020

Biol. plant.

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Gibberellins (GAs) regulate diverse aspects of growth and development, but their role in root development and lateral root (LR) formation is poorly understood. In this study, GA3 was applied to soybean [Glycine max (L.) Merr] by seed soaking. The results showed that root length and root surface area were significantly inhibited in early stages after GA3 treatment. Microscopic examination showed that GA3 treatment changed the cortex thickness, the pericycle diameter, and cell size in main root. Interestingly, exogenous GA3 increased the quantity of lateral root primordia (LRP), but LR number decreased in this period. Moreover, the content of GAs, auxin and abscisic acid in root was altered. RNA-seq results revealed that application of GA3 not only changed the expression of genes in GA biosynthesis pathway, including GA20ox and GA2ox, but also the GA regulation genes and signalling pathway genes. The changes in expression of gene concerning other hormones were also detected. In addition, GA3 altered cell wall biogenesis and degradation genes which might be related to the changes of root morphology. In response to increased GA3, 103 transcription factors were detected. Thus, exogenous GA3 changed the content of hormones in roots and affected the root development by regulating the expression of respective genes.

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“…The major oxidase gene of GA families was all detected after uniconazole treatment (Han et al, 2017). The major oxidase gene of GA families was all detected after uniconazole treatment (Han et al, 2017).…”

Section: Discussionmentioning

confidence: 94%

Gene expression profile indicates involvement of uniconazole in Coix lachryma‐jobi L. seedlings at low temperature

Yu-lan

Yue

Xiang

et al. 2019

Food Science & Nutrition

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Uniconazole (UNZ) can alleviate a variety of abiotic stresses such as low temperature. With application of UNZ on Coix lachryma‐jobi L. (coix) under low‐temperature stress, growth and physiological parameters were investigated in seedlings. Meanwhile, transcriptome profile in coix seedlings was characterized as well. The results showed an increase of 11.90%, 13.59%, and 10.98% in stem diameter, the aboveground and belowground biomass in 5 mg/L uniconazole application group (U3), compared with control check low‐temperature group (CKL). Some anti‐oxidase activities also show significant difference between CKL and U3 (p < .05). Transcriptome results showed that 3,901 and 1,040 genes had different expression level at control check (CK) and CKL, CKL and U3. A considerable number of different expressing genes (DEGs) related to the plant hormone signal transduction, photosynthesis, reactive oxygen species (ROS)‐related genes, and secondary metabolism in response to uniconazole application were identified in this study. The transcriptomic gene expression profiles present a valuable genomic tool to improve studying the molecular mechanisms underlying low‐temperature tolerance in coix. At the same time, it would provide a certain basis for the application of UNZ in the production of coix resistance under low temperature.

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Genome-wide transcriptome profiling reveals the mechanism of the effects of uniconazole on root development in Glycine Max

Cited by 17 publications

References 66 publications

Reinvigoration of diploid strawberry (Fragaria vesca) during adventitious shoot regeneration

Reinvigoration of diploid strawberry (Fragaria vesca) during adventitious shoot regeneration

Treatment of Glycine max seeds with gibberellins alters root morphology, anatomy, and transcriptional networks

Gene expression profile indicates involvement of uniconazole in Coix lachryma‐jobi L. seedlings at low temperature

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