2017
DOI: 10.4014/jmb.1708.08027
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Genomic Approaches for Understanding the Characteristics of Salmonella enterica subsp. enterica Serovar Typhimurium ST1120, Isolated from Swine Feces in Korea

Abstract: subsp. serovar Typhimurium, one of the most common foodborne pathogens, is transmitted mainly through contaminated food derived from infected animals. In this study, Typhimurium ST1120, an isolate from pig feces in Korea, was subjected to whole-genome analysis to understand its genomic features associated with virulence. The genome of ST1120 was found to have a circular chromosome of 4,855,001 bp (GC content 52.2%) and a plasmid of 6,863 bp (GC content 46.0%). This chromosome was predicted to have 4,558 open r… Show more

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Cited by 12 publications
(19 citation statements)
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“…The bacterial strains and plasmids used in this study are listed in Table S1. The wild-type strain Salmonella enterica serovar Typhimurium 1120 (ST1120) isolated in Korea was used as a control strain in all experiments (17). In the animal challenge experiments, ST2173, a S. Typhimurium 14028 strain transformed with the plasmid pBBR1-MCS4 was used (18) containing an ampicillin resistance gene.…”
Section: Bacterial Strains Plasmids and Growth Conditionsmentioning
confidence: 99%
“…The bacterial strains and plasmids used in this study are listed in Table S1. The wild-type strain Salmonella enterica serovar Typhimurium 1120 (ST1120) isolated in Korea was used as a control strain in all experiments (17). In the animal challenge experiments, ST2173, a S. Typhimurium 14028 strain transformed with the plasmid pBBR1-MCS4 was used (18) containing an ampicillin resistance gene.…”
Section: Bacterial Strains Plasmids and Growth Conditionsmentioning
confidence: 99%
“…Typhimurium ST1539 was isolated from a duck slaughterhouse located in Yangpyeong, Gyeong-gi Province in Korea. S. Typhimurium ST1120 previously reported in our study was isolated from feces of pig [12]. All S. Typhimurium strains including LT2 [13], 14028s [14], and SL1344 [15] were grown in Luria Bertani (LB) broth (Difco, USA) or Mueller Hinton broth (Difco) at 37°C with shaking at 220 rpm.…”
Section: Bacterial Strains and Growth Conditionsmentioning
confidence: 99%
“…Salmonella cells cultivated in LB broth overnight were washed and resuspended in DMEM broth, and then added to HeLa cells at a multiplicity of infection (MOI) of 100. At 6 h postinfection, bacterial cytotoxicity was evaluated using CytoTox-ONE Homogeneous Membrane Integrity Assay (Promega) according to the manufacturer's instructions as described in the previous study [12]. LDH released from damaged HeLa cells converts lactate to pyruvate and produces NADH, which in turn converts resazurin to the fluorescent compound resorufin.…”
Section: Lactate Dehydrogenase (Ldh) Cytotoxicity Assaymentioning
confidence: 99%
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“…It can infect both animals and humans and cause various symptoms including fever, diarrhea, nausea, vomiting, and even death [1]. According to recent studies, 93.8 million cases of salmonellosis occur every year, resulting in 155,000 deaths annually [2]. Deaths due to Salmonella infection occur worldwide, regardless of nationality, including in the United States, the United Kingdom, Australia, and Africa [3].…”
Section: Introductionmentioning
confidence: 99%