Genomic attributes and recent advances in detection of white spot syndrome virus in shrimp: a review

Hossain, Nazmul; Chowdhury, Tanjia Afrin; Hasan, Mahmudul; Azim, Kazi Faizul; Jenny, Sayadatunnasa; Rabbee, Muhammad Fazle; Rahman, Mohammad Mafizur; Somana, Saneya Risa

doi:10.1080/17451000.2021.2023191

Cited by 4 publications

(2 citation statements)

References 155 publications

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“…aquatic disease, on-site detection, PfAgo, RPA, WSSV As there is currently no effective treatment for this disease, developing early detection technologies for WSSV is of great significance for the prevention of WSD (Hossain et al, 2022) (Jang et al, 2009;Jaroenram et al, 2009;Mendoza-Cano & Sánchez-Paz, 2013;Mrotzek et al, 2010;Natividad et al, 2008;Xia et al, 2014;Zhang et al, 2022). For convenient detection in the field, isothermal amplification technologies, LAMP and RPA, with the aid of CRISPR-Cas nuclease, have provided on-site detection tools for WSSV (Chaijarasphong et al, 2019;Chen et al, 2021;Wang et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

“…Since its first report in 1992, WSD has caused huge economic losses worldwide (Bhoomaiah et al, 2020; Bir et al, 2017; Stentiford et al, 2012). As there is currently no effective treatment for this disease, developing early detection technologies for WSSV is of great significance for the prevention of WSD (Hossain et al, 2022). With early detection of WSSV in the farming facility, isolation of infected shrimps and/or disinfection of environment can be conducted in time to avoid outbreaks and reduce yield losses.…”

Section: Introductionmentioning

confidence: 99%

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A recombinase polymerase amplification and Pyrococcus furiosus Argonaute combined method for ultra‐sensitive detection of white spot syndrome virus in shrimp

Wang,

Chen,

Tang

et al. 2023

Journal of Fish Diseases

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White spot disease (WSD) in shrimp is an acute infectious disease caused by white spot syndrome virus (WSSV). WSD has seriously threatened the security of shrimp farming, causing huge economic losses worldwide. As there is currently no effective treatment for WSD, developing early detection technologies for WSSV is of great significance for the prevention. In this study, we have established a detection method for WSSV using a combination of recombinase polymerase amplification (RPA) and Pyrococcus furiosus Argonaute (PfAgo). We have achieved a detection sensitivity of single copy per reaction, which is more sensitive than the previously reported detection methods. Additionally, we have demonstrated high specificity. The entire detection process can be completed within 75 min without the need for precise thermal cyclers, making it suitable for on‐site testing. The fluorescence signal generated by the reaction can be quantified using a portable fluorescence detector or observed with the naked eye under a blue light background. This study provides an ultrasensitive on‐site detection method for WSSV in shrimp aquaculture and expands the application of PfAgo in the field of aquatic disease diagnosis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A recombinase polymerase amplification and Pyrococcus furiosus Argonaute combined method for ultra‐sensitive detection of white spot syndrome virus in shrimp

Wang,

Chen,

Tang

et al. 2023

Journal of Fish Diseases

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Portable DNA extraction and loop-mediated isothermal amplification (LAMP) for the on-site detection of white spot syndrome virus (WSSV)

Sathish Kumar,

Suvetha,

Rajeshwar

et al. 2025

Aquaculture

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A colorimetric multiplex RPA approach for on-site dual monitoring of <i>Vibrio parahaemolyticus</i> and White Spot Syndrome Virus in Pacific Whiteleg shrimp

Tran,

Giang,

Phung

et al. 2024

Vietnam J. Biotechnol.

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White spot syndrome virus (WSSV) and Vibrio parahaemolyticus represent the most prevalent and serious pathogens in Viet Nam's shrimp industry, annually inflicting major production losses. With no existing therapeutic measures, timely and accurate diagnosis is imperative for curbing outbreaks and limiting economic impacts through isolation and culling protocols. However, conventional techniques like polymerase chain reaction (PCR) and quantitative PCR require advanced laboratory infrastructure that is incompatible with rapid on-site pathogen surveillance. This work details the development of a multiplex recombinase polymerase amplification (RPA) assay for the simultaneous detection of WSSV and V. parahaemolyticus using a visual colorimetric readout amenable to field deployment. Primers targeting unique WSSV and V. parahaemolyticus sequences were designed for selective amplification. Specificity screening verified exclusive pathogen detection against common shrimp microbiota and human-handling contaminants with no cross-reactivity. Singleplex reactions identified 39 °C for 35 minutes as optimal conditions; hence, these parameters were subsequently applied in multiplex format. Incorporation of the Mg2+-sensitive eriochrome black T (EBT) dye enabled clear discrimination between positive blue and negative violet reactions by the naked eye. The multiplex assay demonstrated high analytical sensitivity, down to 1 copy of DNA template per reaction. Clinical validation of the multiplex RPA method using shrimp samples versus quantitative PCR showed 100% agreement. By coupling rapid isothermal amplification with visual indicator-based detection in a multiplexed format, this assay provides simple, robust, and user-friendly identification of major shrimp pathogens in resource-limited settings in less than one hour to facilitate prompt on-site disease control decisions. The field-suitable platform aims to mitigate outbreak magnitude and economic consequences through timely response.

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Genomic attributes and recent advances in detection of white spot syndrome virus in shrimp: a review

Cited by 4 publications

References 155 publications

A recombinase polymerase amplification and Pyrococcus furiosus Argonaute combined method for ultra‐sensitive detection of white spot syndrome virus in shrimp

A recombinase polymerase amplification and Pyrococcus furiosus Argonaute combined method for ultra‐sensitive detection of white spot syndrome virus in shrimp

Portable DNA extraction and loop-mediated isothermal amplification (LAMP) for the on-site detection of white spot syndrome virus (WSSV)

A colorimetric multiplex RPA approach for on-site dual monitoring of <i>Vibrio parahaemolyticus</i> and White Spot Syndrome Virus in Pacific Whiteleg shrimp

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