Genomic characterization and probiotic potential of
            <i>Lactobacillus casei</i>
            IDCC 3451 isolated from infant faeces

Shin, Minsoo; Ban, O‐Hyun; Jung, Young Hoon; Yang, Jungwoo; Kim, Young-Hoon

doi:10.1111/lam.13449

Cited by 7 publications

(7 citation statements)

References 49 publications

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“…Meanwhile, abundant scientific studies involving the selection of LABs with different and specific functional properties have been reported in the last decades, and new probiotic LABs are also being constantly isolated and identified ( De Melo Pereira et al, 2018 ). These new LABs are isolated from multiple sources, such as human raw milk ( Shin et al, 2021 ) and grains ( Fiorda et al, 2017 ), but milk and other dairy products are commonly considered the main sources of LABs ( Plessas et al, 2017 ; Reuben et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk

et al. 2022

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There are massive bacteria in the raw milk, especially the lactic acid bacteria (LABs), which have been considered probiotics in humans and animals for a long time. Novel probiotics are still urgently needed because of the rapid development of the probiotic industry. To obtain new LABs with high probiotic potential, we obtained 26 LAB isolates, named L1 ~ L26, from local Holstein raw milk collected from a farm whose milk had never been used for LAB isolation. We identified them at the species level by biochemical and 16S rDNA sequencing methods. Their antagonistic activities against four target pathogens (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa PAO1, and Salmonella enterica H9812), co-aggregative ability with these target pathogens, survivability in the simulated gastrointestinal tract conditions and phenol, auto-aggregation and hydrophobicity, hemolytic activity, and antibiotic susceptibility, were evaluated in vitro. Five Lactiplantibacillus plantarum isolates (L5, L14, L17, L19, and L20) showed more promising probiotic potential than others. Specifically, these five isolates conglutinated with and inhibited all the target pathogens, and survived in the simulated gastric juice (92.55 ~ 99.69%), intestinal juice (76.18 ~ 83.39%), and 0.4% phenol (76.95 ~ 88.91%); possessed considerable auto-aggregation (83.91 ~ 90.33% at 24 h) and hydrophobicity (79.32 ~ 92.70%); and were non-hemolytic, sensitive to kinds of common antimicrobials. Our findings demonstrated that these five isolates could be preliminarily determined as probiotic candidates because they have better probiotic potential than those previously reported. Again, this study highlighted the potential of raw milk for probiotic isolating and screening and provided the probiotic industry with five new LAB candidates.

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Section: Introductionmentioning

confidence: 99%

Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk

et al. 2022

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“…To identify probiotic strains with anti-tyrosinase activity, we evaluated the tyrosinase activities of the culture supernatants from seven commercial Lactobacillus and Bifidobacterium strains that are widely used as probiotic supplements ( Shin et al, 2021 ). Most of the strains inhibited the monophenolase activity of tyrosinase, except L. rhamnosus ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Probiotic strains used in this study were kindly gifted from Ildong Bioscience. Bacterial cultures were prepared as previously reported in ( Shin et al, 2021 ). Specifically, probiotic strains ( Lacticaseibacillus rhamnosus IDCC 3201, Lactobacillus acidophilus IDCC 3302, Lacticaseibacillus paracasei IDCC 3401, Lactiplantibacillus plantarum IDCC 3501, Bifidobacterium bifidum IDCC 4201, and Bifidobacterium breve IDCC 4401) were anaerobically cultured at a cell density at 10 9 CFU/mL in De Man, Rogosa and Sharpe medium (MRS; BD Difco, Franklin Lakes, NJ, USA) for 18 h at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Investigation of phenyllactic acid as a potent tyrosinase inhibitor produced by probiotics

Shin

Truong

Lee³

et al. 2023

Current Research in Food Science

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“…The expression of antibiotic resistance in L. paracasei IDCC 3401 is an important safety criterion as the antibiotic resistance of probiotics can be transferred to commensal bacteria or pathogens [ 30 ]. L. paracasei IDCC 3401 was found to be susceptible to all the antibiotics tested, except for kanamycin ( Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

“…Because BA or D-lactate produced by bacteria poses health risks to humans [ 30 ], the ability of L. paracasei IDCC 3401 to produce these toxic compounds was analyzed in the supernatants ( Table 2 ). BAs such as tyramine, histamine, putrescine, 2-phenethylamine, cadaverine, and tryptamine were investigated but were not detected in the supernatants.…”

Section: Resultsmentioning

confidence: 99%

Isolation, Characterization, and Safety Evaluation of the Novel Probiotic Strain Lacticaseibacillus paracasei IDCC 3401 via Genomic and Phenotypic Approaches

Lee,

Bang,

Shin

et al. 2023

Microorganisms

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This study aimed to explore the safety and properties of Lacticaseibacillus paracasei IDCC 3401 as a novel probiotic strain via genomic and phenotypic analyses. In whole-genome sequencing, the genes associated with antibiotic resistance and virulence were not detected in this strain. The minimum inhibitory concentration test revealed that L. paracasei IDCC 3401 was susceptible to all the antibiotics tested, except for kanamycin. Furthermore, the strain did not produce toxigenic compounds, such as biogenic amines and D-lactate, nor did it exhibit significant toxicity in a single-dose acute oral toxicity test in rats. Phenotypic characterization of carbohydrate utilization and enzymatic activities indicated that L. paracasei IDCC 3401 can utilize various nutrients, allowing it to grow in deficient conditions and produce health-promoting metabolites. The presence of L. paracasei IDCC 3401 supernatants significantly inhibited the growth of enteric pathogens (p < 0.05). In addition, the adhesion ability of L. paracasei IDCC 3401 to intestinal epithelial cells was found to be as superior as that of Lacticaseibacillus rhamnosus GG. These results suggest that L. paracasei IDCC 3401 is safe for consumption and provides health benefits to the host.

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Genomic characterization and probiotic potential of Lactobacillus casei IDCC 3451 isolated from infant faeces

Cited by 7 publications

References 49 publications

Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk

Screening and evaluation of lactic acid bacteria with probiotic potential from local Holstein raw milk

Investigation of phenyllactic acid as a potent tyrosinase inhibitor produced by probiotics

Isolation, Characterization, and Safety Evaluation of the Novel Probiotic Strain Lacticaseibacillus paracasei IDCC 3401 via Genomic and Phenotypic Approaches

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