Genomic characterization, kinetics, and pathways of sulfamethazine biodegradation by Paenarthrobacter sp. A01

Cao, Lijia; Zhang, Jiayu; Zhao, Renxin; Deng, Yu; Liu, Jie; Fu, Wenjie; Lei, Yusha; Zhang, Tong; Li, Xiaoyan; Li, Bing

doi:10.1016/j.envint.2019.104961

Cited by 69 publications

(22 citation statements)

References 70 publications

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“…During the enrichment, the increase of Hyphomicrobium, Mizugakiibacter, Rhizomicrobium, Ferruginibacter and Paenarthrobacter was remarkable. Interestingly, it was partly different from the research result of Cao [38]. After screening for high concentration of SM2, Paenarthrobacter and Ferruginibacter also appeared in this system, but other bacteria showed differences: Aridibacter, Geothrix, Plasticicumulans, and Terrimonas did not become dominant genera, but instead Hyphomicrobium, Mizugakiibacter and Rhizomicrobium Were dominant.…”

Section: Discussioncontrasting

confidence: 85%

Biodiversity, isolation and genome analysis of sulfamethazine-degrading bacteria using high-throughput analysis

Wang

et al. 2020

Bioprocess Biosyst Eng

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Sulfamethazine (SM2) is one of the sulfonamide antibiotics that is frequently detected in aquatic environment. Given the complex structure of SM2 and its potential threat to the environment, it is necessary to determine the degradation behavior of high-concentration SM2. The mechanisms of community structure and diversity of activated sludge were analyzed. A novel SM2-degrading strain YL1 was isolated which can degrade SM2 with high concentration of 100 mg L −1. Strain YL1 was identified as Paenarthrobacter ureafaciens and there was also a significant increase in the genus during acclimation. Additional SM2 metabolic mechanisms and genomic information of YL1 were analyzed for further research. The succession of the community structure also investigated the effect of SM2 on the activated sludge. This result not only advances the current understanding of microbial ecology in activated sludge, but also has practical implications for the design and operation of the environmental bioprocesses for treatment of antimicrobial-bearing waste streams.

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Section: Discussioncontrasting

confidence: 85%

Biodiversity, isolation and genome analysis of sulfamethazine-degrading bacteria using high-throughput analysis

Wang

et al. 2020

Bioprocess Biosyst Eng

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“…A mineral medium (MM) containing 1.5 g/L K 2 HPO 4 , 0.5 g/L KH 2 PO 4 , 0.2 g/L MgSO 4 , and 1.5 g/L NH 4 Cl was used for the enrichment of estrogen-degrading strains (Cao et al, 2019). A Luria-Bertani medium (LB) containing 10 g/L tryptone, 5 g/L yeast extract, and 10 g/L NaCl was used for the cultivation of the strain CU-2, and strain LC-1 was cultivated with a minimal salt medium (MSM), which was composed of 10 g/L casein peptone, 10 g/L beef extract, 5 g/L yeast extract, 5 g/L glucose, 5 g/L sodium acetate, 2 g/L diamine citrate, 1 g/L Tween 80, 2 g/L K 2 HPO 4 , 0.2 g/L MgSO 4 •7H 2 O, 0.05 g/L MnSO 4 •H 2 O, and 20 g/L CaCO 3 .…”

Section: Chemicals and Materialsmentioning

confidence: 99%

A Comparative Study on the Biodegradation of 17β-Estradiol by Candida utilis CU-2 and Lactobacillus casei LC-1

Yang

et al. 2021

Front. Energy Res.

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The release and fate of estrogens have attracted more and more public attention. Biodegradation is an important method for estrogen removal from the environment. However, few comparative studies concentrated on the degradation of 17β-estradiol (E2) by fungi and bacteria. In this study, the removal efficiencies of E2 by fungi (Candida utilis CU-2) and bacteria (Lactobacillus casei LC-1) were investigated through influencing factors, kinetics, and biodegradation pathways. The results demonstrated that both C. utilis CU-2 and L. casei LC-1 have the same degradation efficiency, and they can effectively degrade E2 (10 μM) with nearly 97% degradation efficiency. However, the biodegradation efficiency of the two strains only reached 20% when E2 was used as a sole carbon source, while it increased to 97% with 1.2 g/L sucrose, glucose, or sodium acetate supply, indicating the occurrence of co-metabolism. In addition, the results indicated that 35°C and 0.6 g/L sucrose favored the degradation. However, the addition of excessive carbon sucrose (10 g/L) significantly inhibited the biodegradation of E2. Besides, the degradation of E2 with ~0–10 g/L sucrose as co-substrate followed the first-order kinetics well. Through intermediate products analysis, 12 degradation products were identified, and they were mainly produced via hydroxylation and methylation, among others, among which C14H22O4 (m/z:[M + H]+ = 255) was detected as the product with the smallest amount of carbon in this study. Based on the detected products and previous studies, five biodegradation pathways were proposed. To our knowledge, there are few reports about the comparisons of E2 removal between fungi and bacteria. Moreover, the results confirmed that the strain CU-2 and the strain LC-1 may have similar degradation characteristics and metabolic mechanisms in the degradation of E2. This study may provide a promising bio-treatment method with low energy consumption for E2 removal from aqueous environments and help in understanding their biodegradation mechanisms.

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“…The degradation of SMX by both isolated and enriched bacteria has been reported [ 26 , 27 , 28 , 29 , 30 , 31 , 32 ]. Co-cultures are superior to monocultures in biodegradation of SMX, because microorganisms can cooperate with each other to remove antibiotics [ 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

Harnessing Paenarthrobacter ureafaciens YL1 and Pseudomonas koreensis YL2 Interactions to Improve Degradation of Sulfamethoxazole

Wang

Shan

et al. 2022

Microorganisms

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Sulfamethoxazole (SMX) is a widespread and persistent pollutant in the environment. Although the screening and analysis of SMX-degrading bacteria have been documented, the interaction mechanisms of functional microorganisms are still poorly understood. This study constructed a consortium with strain YL1 and YL2 supplied with SMX as the sole carbon and energy source. The coexisting mechanism and the removal of SMX of the consortium were investigated. The total oxidizable carbon (TOC) removal rate of the combined bacterial system was 38.94% compared to 29.45% for the single bacterial system at the same biomass. The mixed bacterial consortium was able to resist SMX at concentrations up to 400 mg/L and maintained a stable microbial structure at different culture conditions. The optimum conditions found for SMX degradation were 30 °C, pH 7.0, a shaking speed of 160 r·min−1, and an initial SMX concentration of 200 mg·L−1. The degradation of SMX was accelerated by the addition of YL2 for its ability to metabolize the key intermediate, 4-aminophenol. The removal rate of 4-aminophenol by strain YL2 reached 19.54% after 5 days. Genome analysis revealed that adding riboflavin and enhancing the reducing capacity might contribute to the degradation of SMX. These results indicated that it is important for the bioremediation of antibiotic-contaminated aquatic systems to understand the metabolism of bacterial communities.

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Genomic characterization, kinetics, and pathways of sulfamethazine biodegradation by Paenarthrobacter sp. A01

Cited by 69 publications

References 70 publications

Biodiversity, isolation and genome analysis of sulfamethazine-degrading bacteria using high-throughput analysis

Biodiversity, isolation and genome analysis of sulfamethazine-degrading bacteria using high-throughput analysis

A Comparative Study on the Biodegradation of 17β-Estradiol by Candida utilis CU-2 and Lactobacillus casei LC-1

Harnessing Paenarthrobacter ureafaciens YL1 and Pseudomonas koreensis YL2 Interactions to Improve Degradation of Sulfamethoxazole

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