Genomic circular RNA expression profile analysis indicated hsa_circRNA_000780 as a diagnostic marker for gastric cancer

Abstract

Background: This study aimed to find the specific circular RNA (circRNA) for gastric cancer (GC) and lay the foundation for the early diagnosis of GC.Methods: Four patients with GC were selected for this study. The total RNA of their cancer tissues and adjacent tissues was extracted, and the circRNA was screened. The top eight upregulated and downregulated circRNAs with statistical significance between GC and paired adjacent nontumorous tissues were identified using real-time fluorescent quantitative polymerase chain reaction (PCR). The expression level of circRNA was identified by quantitative reverse transcription PCR in 78 cases of GC and its adjacent tissues, and in the gastric juice of 30 patients with chronic nonatrophic gastritis, 30 with chronic atrophic gastritis, 21 with early GC, and 57 with advanced GC.Results: A total of 445 circRNAs, including 69 upregulated and 376 downregulated circRNAs, were found to be significantly aberrantly expressed in GC tissues. Most of the differentially expressed circRNAs originated from chr1, chr3, chr4, chr6, and chr11. Hsa_circRNA_000780 was significantly downregulated in 80.77% of GC tissues. Its level in GC tissues correlated with the tumor size, tumor stage, T stage, venous invasion, carcinoembryonic antigen, and carbohydrate antigen 19-9 expression. More importantly, hsa_circRNA_000780 was found in the gastric juice of early and advanced GC.Conclusions: This study uncovered the new hsa_circRNA expression profile in human GC. Among them, hsa_circRNA_000780 was significantly downregulated in GC tissues and gastric juice. It had the potential to be used as a novel biomarker for the screening of early GC.

Degradation Dynamics and Residue Analysis of Four Propiconazole Stereoisomers in “Fengtang” Plum during Storage by LC-MS/MS

“…"Fengtang" plum is a kind of plum cultivated in Guizhou, China, which enjoys the title of geographical landmark agricultural product [1]. It is also one of the fruit crops with the largest planting area in Guizhou, China, the "Fengtang" plum planting area as of 2019 was approximately 30,000 hm 2 , and the planting area is constantly expanding [2]. "Fengtang" plum is favored by many consumers because it is rich in vitamins, sugars, phenolics, flavonoids, and other nutrients needed by humans, the total sugar content of mature "Fengtang" plum was as high as 82.65 mg•g −1 FW [3,4].…”