Genomic Classification and Genetic Relationships of a New Variant of Hepatitis A Virus Isolated in Cuba

Díaz, Belkis; Sariol, Carlos A.; Martínez, Ricardo Montero; Rodríguez, Licel

doi:10.1590/s0074-02761999000300013

Cited by 2 publications

(1 citation statement)

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“…(AB038292, AB038299, AB038300, AB038305; unpublished); CR326 (Linemeyer et al, 1985); RJ-PMC3, RJ-NSG2, RJ504, RJ-193, RJ-907, RJ-PMC1 (De Paula et al, 2002); GBM/WT (Graff et al, 1994a); KU98-42, KU98-D2 (Byun et al, 2001); Arg578 (Mbayed et al, 2002); EP35-730, EP49-674, H-141, KPH, CF53/Berne, H-174, H209, H217, H229, SMA292, TK-23, PA21, JM-55, India90, A-177, M-25, A-162 (Robertson et al, 1992); HAP009, HAP039 (AF538723, AF538724; unpublished); Nor-7, Nor-11, Nor-13 (Grinde et al, 1997); Nor-18, Nor-19, Nor-20, Nor-21, Nor-23, Nor-24 (Stene-Johansen et al, 1999); RJ-213 (De Paula et al, 2003); CU236-F (Diaz et al, 2001); SLC1697 (Hutin et al, 1999); IT-VIO-01, IT-LAF-00 (AJ505577, AJ505624; unpublished); SA/10/2000/DE (AY028976; unpublished); HAS15 (Sverdlov et al, 1987); M2 (Diaz et al, 1999); A39 (AB046901; unpublished); MBB (Paul et al, 1987); 412991 and 504184 (Taylor, 1997); HM-175 (Cohen et al, 1987); SLF88 (Ching et al, 2002); CY145 (Nainan et al, 1991); A'dam063 (AY101269; unpublished); AGM27 (Tsarev et al, 1991); and GA76 (Robertson et al, 1992;Khanna et al, 1992).…”

Section: Phenotypic Characteristics: Amino Acid Changes and Cell-cultmentioning

confidence: 99%

Characterization of the complete genomic sequence of genotype II hepatitis A virus (CF53/Berne isolate)

Lü

Ching

Paula

et al. 2004

Journal of General Virology

103

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The complete genomic sequence of hepatitis A virus (HAV) CF53/Berne strain was determined. Pairwise comparison with other complete HAV genomic sequences demonstrated that the CF53/Berne isolate is most closely related to the single genotype VII strain, SLF88. This close relationship was confirmed by phylogenetic analyses of different genomic regions, and was most pronounced within the capsid region. These data indicated that CF53/Berne and SLF88 isolates are related more closely to each other than are subtypes IA and IB. A histogram of the genetic differences between HAV strains revealed four separate peaks. The distance values for CF53/Berne and SLF88 isolates fell within the peak that contained strains of the same subtype, showing that they should be subtypes within a single genotype. The complete genomic data indicated that genotypes II and VII should be considered a single genotype, based upon the complete VP1 sequence, and it is proposed that the CF53/Berne isolate be classified as genotype IIA and strain SLF88 as genotype IIB. The CF53/Berne isolate is cell-adapted, and therefore its sequence was compared to that of two other strains adapted to cell culture, HM-175/7 grown in MK-5 and GBM grown in FRhK-4 cells. Mutations found at nucleotides 3889, 4087 and 4222 that were associated with HAV attenuation and cell adaptation in HM175/7 and GMB strains were not present in the CF53/Berne strain. Deletions found in the 59UTR and P3A regions of the CF53/Berne isolate that are common to cell-adapted HAV isolates were identified, however.

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Section: Phenotypic Characteristics: Amino Acid Changes and Cell-cultmentioning

confidence: 99%

Characterization of the complete genomic sequence of genotype II hepatitis A virus (CF53/Berne isolate)

Lü

Ching

Paula

et al. 2004

Journal of General Virology

103

View full text Add to dashboard Cite

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Genetic relatedness of Cuban HAV wild‐type isolates

Díaz

Sariol

Normann

et al. 2001

Journal of Medical Virology

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Knowledge of the nucleotide sequence in the region of the putative VP1/2A junction of the Hepatitis A virus (HAV) genome has enabled differentiation of HAV strains and their classification into seven genotypes, in some of which sub-genotypes A and B can be defined. A 168 base segment encompassing the putative VP1/2A junction of 27 clinical wild-type isolates of HAV from Cuba was amplified by reverse transcriptase-polymerase chain reaction and then sequenced. The Cuban isolates are clustered within sub-genotype IA. A single amino acid substitution, which does not correspond with any of the reported changes for other strains, was observed. A new sub-genotype variant is therefore postulated. This study provides new data on the genetic relatedness of HAVs from Cuba and on the distribution of sub-genotype IA in the Caribbean area.

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Genomic Classification and Genetic Relationships of a New Variant of Hepatitis A Virus Isolated in Cuba

Cited by 2 publications

References 0 publications

Characterization of the complete genomic sequence of genotype II hepatitis A virus (CF53/Berne isolate)

Characterization of the complete genomic sequence of genotype II hepatitis A virus (CF53/Berne isolate)

Genetic relatedness of Cuban HAV wild‐type isolates

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