Genomic differences between<i>Actinobacillus pleuropneumoniae</i>serotypes 1 and 3 and the diversity distribution among 15 serotypes

Xie, Fang; Lei, Liancheng; Du, Cheng; Liu, Shuqing; Wang, Han; Ren, Zhijun

doi:10.1111/j.1574-6968.2009.01870.x

Cited by 7 publications

(7 citation statements)

References 32 publications

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“…4 The omlA PCR type of strains FH24-1, FH24-2, and FH24-5 corresponded to that seen in serovars 3, 6-8, 13, and 15. 4 The PCR assays targeting genes for the Wzz protein and VI polysaccharide protein of strains FH24-1, FH24-2, and FH24-5 were positive, as previously seen in serovars 3, 6, and 15 21 and serovars 3, 8, and 15, 21 respectively. We next attempted to amplify the cps loci with PCR primers RV1 and FW1 ( Fig.…”

Section: Other Pcr Resultssupporting

confidence: 67%

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Nucleotide sequence analysis of a DNA region involved in capsular polysaccharide biosynthesis reveals the molecular basis of the nontypeability of two Actinobacillus pleuropneumoniae isolates

et al. 2016

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The aim of our study was to reveal the molecular basis of the serologic nontypeability of 2 Actinobacillus pleuropneumoniae field isolates. Nine field strains of A. pleuropneumoniae, the causative agent of porcine pleuropneumonia, were isolated from pigs raised on the same farm and sent to our diagnostic laboratory for serotyping. Seven of the 9 strains were identified as serovar 15 strains by immunodiffusion tests. However, 2 strains, designated FH24-2 and FH24-5, could not be serotyped with antiserum prepared against serovars 1-15. Strain FH24-5 showed positive results in 2 serovar 15-specific PCR tests, whereas strain FH24-2 was only positive in 1 of the 2 PCR tests. The nucleotide sequence analysis of gene clusters involved in capsular polysaccharide biosynthesis of the 2 nontypeable strains revealed that both had been rendered nontypeable by the action of ISApl1, a transposable element of A. pleuropneumoniae belonging to the IS30 family. The results showed that ISApl1 of A. pleuropneumoniae can interfere with both the serologic and molecular typing methods, and that nucleotide sequence analysis across the capsular gene clusters is the best means of determining the cause of serologic nontypeability in A. pleuropneumoniae.

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Section: Other Pcr Resultssupporting

confidence: 67%

“…4 The O-PS PCR assays targeting genes encoding the Wzz protein and VI polysaccharide biosynthesis protein, which are involved in biosynthesis of lipopolysaccharide O-PS, were performed as described previously. 21…”

Section: Polymerase Chain Reaction Assaysmentioning

confidence: 99%

Nucleotide sequence analysis of a DNA region involved in capsular polysaccharide biosynthesis reveals the molecular basis of the nontypeability of two Actinobacillus pleuropneumoniae isolates

et al. 2016

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“…Furthermore, a Chi-square test (3 × 2) was performed to compare the scores according to the different classes. The classes identified were: slight (0-9), moderate (10)(11)(12)(13)(14)(15)(16)(17)(18)(19), and severe (20)(21)(22)(23)(24)(25)(26)(27)(28) for the lung score; slight (0-2) and severe (3,4) for the pleural score; and slight (0-2) and severe (3)(4)(5) for the nasal score. The Chi-square test (2 × 2) was also performed to compare pleural and nasal scores.…”

Section: Discussionmentioning

confidence: 99%

“…An end-point PCR was performed to confirm the presence of H. parasuis according to the protocol described by Oliveira et al ( 20 ), and A. pleuropneumoniae serotyping was performed according to the protocol described by Xie et al ( 21 ).…”

Section: Methodsmentioning

confidence: 99%

Association Between Infectious Agents and Lesions in Post-Weaned Piglets and Fattening Heavy Pigs With Porcine Respiratory Disease Complex (PRDC)

et al. 2020

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Porcine Respiratory Disease Complex (PRDC) is a multifactorial syndrome that causes health problems in growing pigs and economic losses to farmers. The etiological factors involved can be bacteria, viruses, or mycoplasmas. However, environmental stressors associated with farm management can influence the status of the animal's health. The role and impact of different microorganisms in the development of the disease can be complex, and these are not fully understood. The severity of lesions are a consequence of synergism and combination of different factors. The aim of this study was to systematically analyse samples, conferred to the Veterinary Diagnostic Laboratory (IZSLER, Brescia), with a standardized diagnostic protocol in case of suspected PRDC. During necropsy, the lungs and carcasses were analyzed to determine the severity and extension of lesions. Gross lung lesions were classified according to a pre-established scheme adapted from literature. Furthermore, pulmonary, pleural, and nasal lesions were scored to determine their severity and extension. Finally, the presence of infectious agents was investigated to identify the microorganisms involved in the cases studied. During the years 2014-2016, 1,658 samples of lungs and carcasses with PRDC from 863 farms were analyzed; among them 931 and 727 samples were from weaned piglets and fattening pigs, respectively. The most frequently observed lesions were characteristic of catarrhal bronchopneumonia, broncho-interstitial pneumonia, pleuropneumonia, and pleuritis. Some pathogens identified were correlated to specific lesions, whereas other pathogens to various lesions. These underline the need for the establishment of control and treatment programmes for individual farms.

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“…However, it is difficult to carry out more general studies of the immunity mechanisms of different serotypes, typing-based diagnosis, and multivalent genetically engineered vaccines due to the lack of complete genome sequences of the different serotypes (11).…”

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confidence: 99%

Draft Genome Sequences of Actinobacillus pleuropneumoniae Serotypes 2 and 6

et al. 2010

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Actinobacillus pleuropneumoniae is a bacterial pathogen that causes highly contagious respiratory infection in pigs and has a serious impact on the production economy and animal welfare. As clear differences in virulence between serotypes have been observed, the genetic basis should be investigated at the genomic level. Here, we present the draft genome sequences of the A. pleuropneumoniae serotypes 2 (strain 4226) and 6 (strain Femo).Previous studies of different serotypes of Actinobacillus pleuropneumoniae showed that there are significant variations among them at the DNA sequence level, supposed to cause differences in pathogenicity and immunogenicity (2,8). However, it is difficult to carry out more general studies of the immunity mechanisms of different serotypes, typing-based diagnosis, and multivalent genetically engineered vaccines due to the lack of complete genome sequences of the different serotypes (11).Draft genome sequences of serotypes 2 and 6 were assembled by combining Roche 454-FLX reads with Illumina Genome Analyzer IIx paired-end reads. The final assembly (CLCGenomicsWorkbench version 3.6, CLCbio) of the serotype 2 genome has a length of 2,314,315 bp (38 contigs), while the assembly of serotype 6 has a length of 2,375,501 bp (36 contigs). The average GC contents are 41.17% and 40.95% in serotypes 2 and 6, respectively, similar to those reported for other serotypes (1, 12). Using EasyGene (6, 9), 2,100 putative open reading frames were predicted for serotype 2 and 2,168 for serotype 6. Approximately 86% of the nucleotides were predicted to be involved in coding sequences, which is similar to results reported for other finished A. pleuropneumoniae genomes (1, 12). There are 86 and 136 genes found to be specific for serotypes 2 and 6, respectively, that do not have any homologue in other reported serotypes. rRNA genes were identified by RNAmmer (5). Serotype 2 harbors six rRNA operons (16S-23S-5S rRNA) and three additional 5S rRNA genes, while six rRNA operons and one additional 5S rRNA gene are present in serotype 6. Using the tRNAscan-SE server (7), 61 and 59 tRNA operons were predicted for serotypes 2 and 6, respectively.Since porcine pleuropneumonia caused by A. pleuropneumoniae leads to large economic losses for the swine industry (3), it is of considerable interest to investigate the virulence factors of the different serotypes. We compiled a list of 105 known and putative virulence genes of A. pleuropneumoniae from published literature and performed a diversity study of these genes at the nucleotide level, comparing serotypes 2 and 6. Sixty-two conserved virulence genes (Ͼ99% identity or fewer than three mismatches) were found between these two serotypes, while 28 virulence genes showed a larger degree of dissimilarity (Ͻ95% identity or more than 20 mismatches), including candidates like the apxIVA gene encoding RTX toxin and the cysI gene encoding NADPH-sulfite reductase hemoprotein. Capsular polysaccharides (CPS) produced by A. pleuropneumoniae are considered to be important virulence f...

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Genomic differences betweenActinobacillus pleuropneumoniaeserotypes 1 and 3 and the diversity distribution among 15 serotypes

Cited by 7 publications

References 32 publications

Nucleotide sequence analysis of a DNA region involved in capsular polysaccharide biosynthesis reveals the molecular basis of the nontypeability of two Actinobacillus pleuropneumoniae isolates

Nucleotide sequence analysis of a DNA region involved in capsular polysaccharide biosynthesis reveals the molecular basis of the nontypeability of two Actinobacillus pleuropneumoniae isolates

Association Between Infectious Agents and Lesions in Post-Weaned Piglets and Fattening Heavy Pigs With Porcine Respiratory Disease Complex (PRDC)

Draft Genome Sequences of Actinobacillus pleuropneumoniae Serotypes 2 and 6

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