Genomic DNA Isolation and Purification of Two Endemic Medicinal Plants (Pterocarpus Santalinus Linn.F & Pimpinella Tirupatiensis Bal&Subr) of Seshachalam Hills, Tirumala.

Abstract

Ptercarpus santalinus is an important endemic medicinal plant with high medicinal values that interfere with DNA extraction procedures and qualitative, quantitative agaros gel electrophoresis. An effective and low-cost protocol for isolating genomic DNA from the roots of Pterocarpus santilinus and Pimpinella tirupatiensis was described in this paper. It involved a modified CTAB method with distilled water pretreating samples. The A 260 /A 280 absorbance ratio of extracted DNA was found to be free from polysaccharide, poly-phenols and tannins contaminants ranged from 2.2 to 2.8 within the high level of purity.