Genomic features of a multidrug-resistant Enterobacter cloacae ST279 producing CTX-M-15 and AAC(6′)-Ib-cr isolated from fatal infectious stomatitis in a crossed pit viper (Bothrops alternatus)

Carvalho, Marcelo Pires Nogueira de; Moura, Quézia; Fernandes, Miriam R.; Sellera, Fábio P.; Pagotto, Ana Helena; Stuginski, Daniel Rodrigues; Castro, Rafael A.; Sant’Anna, Sávio Stefanini; Grego, Kathleen Fernandes; Lincopán, Nilton

doi:10.1016/j.jgar.2018.11.009

Cited by 2 publications

(1 citation statement)

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“…Studies on findings of MDR bacteria in reptiles have increased in recent years. Among the reasons for conducting this kind of research were proofs for anthropogenic changes, including conservation issues, ecosystem health status, and environmental pollution [ 66 , 73 , 74 ]; veterinary aspects [ 75 , 76 ]; and possible zoonotic infections [ 40 ]. Most studies have been conducted to assess the health situation of reptiles in their ecosystems or when injured reptiles suffered from infection.…”

Section: Discussionmentioning

confidence: 99%

Imported Pet Reptiles and Their “Blind Passengers”—In-Depth Characterization of 80 Acinetobacter Species Isolates

Unger

Eisenberg²,

Prenger‐Berninghoff

et al. 2022

Microorganisms

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Reptiles are popular pet animals and important food sources, but the trade of this vertebrate class is—besides welfare and conservation—under debate due to zoonotic microbiota. Ninety-two shipments of live reptiles were sampled during border inspections at Europe’s most relevant transshipment point for the live animal trade. Acinetobacter spp. represented one significant fraction of potentially MDR bacteria that were further analyzed following non-selective isolation or selective enrichment from feces, urinate, or skin samples. Taxonomic positions of respective isolates were confirmed by MALDI-TOF MS and whole-genome sequencing analysis (GBDP, dDDH, ANIb, and rMLST). The majority of the 80 isolates represented established species; however, a proportion of potentially novel taxa was found. Antimicrobial properties and genome-resistance gene screening revealed novel and existing resistance mechanisms. Acinetobacter spp. strains were most often resistant to 6–10 substance groups (n = 63) in vitro. Resistance to fluorchinolones (n = 4) and colistin (n = 7), but not to carbapenems, was noted, and novel oxacillinase variants (n = 39) were detected among other genes. Phylogenetic analysis (MLST) assigned few isolates to the known STs (25, 46, 49, 220, and 249) and to a number of novel STs. No correlation was found to indicate that MDR Acinetobacter spp. in reptiles were associated with harvesting mode, e.g., captive-bred, wild-caught, or farmed in natural ecosystems. The community of Acinetobacter spp. in healthy reptiles turned out to be highly variable, with many isolates displaying a MDR phenotype or genotype.

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