Genomic gene encoding manganese peroxidase from a white-rot fungus Phanerochaete crassa WD1694

Takano, Mariko; Yamaguchi, Muneysohi; Sano, Hiroaki; Nakamura, Masaya; Shibuya, Haruo; Miyazaki, Yasumasa

doi:10.1007/s10086-012-1309-z

Cited by 5 publications

(5 citation statements)

References 36 publications

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“…Perlakuan pendahuluan bertujuan untuk menghilangkan, memutus atau mengurangi keeratan ikatan antara selulosa dan hemiselulosa dengan lignin. Ikatan lignoselulosa dapat diputus oleh ligninase seperti lignin peroksidase (LiP) dan mangan peroksidase (MnP) (Takano et al, 2004). Enzim LiP dan MnP dihasilkan oleh beberapa organisme termasuk diantaranya Phanerochaete chrysosporium.…”

Section: Pendahuluanunclassified

Performa Kambing yang Diberi Kulit Buah Kakao Terfermentasi

Suparjo¹,

Wiryawan²,

Laconi³

et al. 2011

med pet

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Utilization of cocoa pod husk (CPH) as feedstuff needs pretreatment to increase its nutrients availability. Bioconversion with Phanerochaete chrysosporium changes its structure by breaking down the linkage between lignin and structural carbohydrates. This experiment was aimed to evaluate the quality of fermented CPH biomass as feed for goats. The experimental treatments i.e.: A= 30% of fresh napier grass (RG) + 50% of dried RG + 20% of concentrate; B= 30% of fresh RG + 30% of dried RG + 40% of concentrate; C= 30% of fresh RG + 30% of CPH + 40% of concentrate; D= 30% of fresh RG + 30% of fermented CPH + 40% of concentrate and E= 30% of fresh RG + 50% of fermented CPH + 20% of concentrate. The treatments were allocated in a randomized block design with three replications. Feed intake, body weight gain and ration effi ciency were measured. The use of fermented CPH at the level of 30% had higher (P<0.05) feed intake (560.33 g day -1 ), body weight gain (101.79 g head -1 day -1 ), and feed conversion (5.50) compared to other treatments. In conclusion that the use of 30% fermented CPH in the ration showed the best body weight gain and feed effi ciency.

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Section: Pendahuluanunclassified

Performa Kambing yang Diberi Kulit Buah Kakao Terfermentasi

Suparjo¹,

Wiryawan²,

Laconi³

et al. 2011

med pet

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“…The biosynthesis trend of MnP from AGUM004 and AGUM007 was studied using modified production media (Takano et al 2004). Production media was composed of 0.4g K2HPO4, 0.6 g KH2PO4, 0.5 g MgSO4, 0.03 g MnSO4, 0.01 g CaCO3, 1 g NH4H2PO4, 0.25 g yeast extract, 10 g glucose, 0.5 g Tween 80, and 8.3 g sodium malonate in 1000 mL of distilled water.…”

Section: Mnp Productionmentioning

confidence: 99%

Screening of wild basidiomycetes and evaluation of the biodegradation potential of dyes and lignin by manganese peroxidases

Rao

Ravichandran

Kandalam

et al. 2019

BioRes

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Manganese peroxidase (MnP), a crucial enzyme in biodegradation of lignin, is synthesized by most white rot fungi. To obtain novel enzymes with superior biodegradation potential, MnP-producing wild isolates were evaluated for their ability to degrade recalcitrant azo dyes, sulfonephthalein dyes, and kraft lignin. Of 30 wild isolates screened, 18 tested positive for lignin modifying enzymes (LMEs). Thirteen of these isolates were positive for both laccase and MnP, whereas four produced only laccase, and one produced lignin peroxidase alone. The isolates were identified as Clitopilus scyphoides MH172162 (AGUM004), Ganoderma rasinaceum MH172163 (AGUM007), and three Schizophyllum species: MH172164, MH172165, and MH172166 (KONA001, AGUM0011, and AGUM021). The Fourier-transform infrared spectroscopy (FTIR) analysis of dye degradation and kraft lignin samples with AGUM004 and AGUM007 revealed biotransformation. The former could not completely degrade Reactive Black 5 and bromocresol green, but it could completely (100%) decolorize bromophenyl blue, bromothymol blue, and Remazol brilliant blue R. The latter efficiently degraded almost all tested dyes. Both degraded kraft lignin. The screened hyper MnP-producing wild AGUM004 and AGUM007 were shown to be potential dye degraders in addition to having lignin degrading abilities.

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“…Manganese peroxidase (MnP) activity was measured as described by Takano et al (2004) [14]. The reaction mixture contained 20 mM 2,6-dimethoxyphenol as a substrate, 50 mM malonate buffer (pH 4.5), 20 mM MnSO 4 , and 2 mM H 2 O 2 .…”

Section: Enzyme Activity Analysismentioning

confidence: 99%

Enhanced Culture in Static Batch Systems for Benzo[a]pyrene Degradation by Bjerkandera Adusta SM46 under Acidic and Saline Conditions

Andriani¹,

Tachibana²,

Itoh³

2017

J-SustaiN

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Biodegradation of high-molecular weight polycyclic aromatic hydrocarbons (PAHs) is biologically difficult to degrade due to its recalcitrant characteristics such as low solubility in water and inhibition effect. In the present study, we used an enhanced culture system (ECS) for Bjerkandera adusta SM46 on benzo[a]pyrene (BaP) degradation under acidic (pH 4.5) and saline conditions (pH 8.2). The ECS using Tween 80 (T-80) and MnSO4 enhanced both the ligninolytic enzyme activities and degradation rate by the fungus. The optimum condition was obtained at a concentration for MnSO4 of 0.4-0.9 mM and for T-80 of 0.45-0.70%, which increased degradation more than 87% at day 30 (pH 4.5). The ECS could also be applied under a saline condition for up to 82% degradation. Mangan peroxidase (MnP) and lignin peroxidase (LiP) showed high activities at pH 4.5 (110.9 and 79.9 U/L, respectively). Moreover, laccase and MnP showed higher activities (75.6 and 130.0 U/L, respectively) at saline pH 8.2 indicating stress condition stimulation. * Corresponding AuthorTel.: +81-89-9469864; E-mail: tatibana@agr.ehime-u.ac Using rice straw also enhanced the BaP degradation rate by the fungus via cell growth promotion. This study suggests that an ECS treatment and rice straw co-substrate system could potentially enhance PAH degradation for a static batch system under both an acidic and a salinealkaline stress condition.

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Genomic gene encoding manganese peroxidase from a white-rot fungus Phanerochaete crassa WD1694

Cited by 5 publications

References 36 publications

Performa Kambing yang Diberi Kulit Buah Kakao Terfermentasi

Performa Kambing yang Diberi Kulit Buah Kakao Terfermentasi

Screening of wild basidiomycetes and evaluation of the biodegradation potential of dyes and lignin by manganese peroxidases

Enhanced Culture in Static Batch Systems for Benzo[a]pyrene Degradation by Bjerkandera Adusta SM46 under Acidic and Saline Conditions

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