Genomic identification of the breed of an individual or its tissue

Ciampolini, Roberta; Levéziel, Hubert; Mazzanti, Elisa; Grohs, Cécile; Cianci, D.

doi:10.1016/s0309-1740(99)00061-3

Cited by 32 publications

(16 citation statements)

References 6 publications

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“…In agreement with data reported in different studies with different breeds and markers (Ciampolini et al, 2000;Negrini et al, 2007;Dalvit et al, 2008), the Piemontese breed had the highest percentage of correct assignment (87.6, with 61% of the values exceeding 95%), Lasagna et al …”

Section: Snps For Comparison Of Italian Beef Cattlesupporting

confidence: 90%

Comparison of Four Italian Beef Cattle Breeds by Means of Functional Genes

Lasagna

Ceccobelli

Lorenzo

et al. 2015

Italian Journal of Animal Science

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Piemontese, Chianina, Marchigiana and Romagnola are the main Italian beef breeds, and the quality of their products is largely recognised all over the world. Here, 18 single nucleotide polymorphisms (SNPs) in 12 candidate genes involved on meat traits were investigated on 1055 candidates for selection in order to analyse the within-and betweenbreed variability with a functional marker approach. Three SNPs (GDF8-3, GH and NPY-3) were monomorphic and most of the polymorphic SNPs showed an allele distribution quite similar in the four breeds. High variability at LEP-2, LEP-3 and LEPR markers was detected across breeds and the analysis of the relationship between genetic differentiation and heterozygosity indicated significant deviation from a neutral-equilibrium model for LEP-2. The highest pairwise fixation index values (0.1189 to 0.1877) were obtained for the comparisons of Piemontese with the other breeds, while the lowest value (0.0296) was observed in Chianina and Marchigiana. The Piemontese differentiation from the other breeds could be due to its geographical isolation and selection targets. The results for breed assignment follows the genetic differentiation, in fact, Piemontese had the highest percentage of correct assignment (87.6), while Marchigiana had the lowest (47.5). These findings suggest that functional markers can be more suitable than neutral markers in discriminating breeds similar in morphology if selection plays some role in their differentiation.

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Section: Snps For Comparison Of Italian Beef Cattlesupporting

confidence: 90%

Comparison of Four Italian Beef Cattle Breeds by Means of Functional Genes

Lasagna

Ceccobelli

Lorenzo

et al. 2015

Italian Journal of Animal Science

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“…This allows one to validate the genetic origin of highly prized Iberian meat products, because the problem can be reduced to a one-to-one breed comparison. 3 The contribution of other improved breeds (through crossbreeding with Iberian pigs) is discarded, because these breeds are not adequate for the hard climate conditions of the extensive production system and for the high-quality requirements of the meat and fat. 5 The two coat colour genes analysed (MC1R and OCA2) presented breed specific alleles.…”

Section: Discussionmentioning

confidence: 99%

“…Some DNA tests have been proposed for discriminating among European cattle breeds. 2,3 The production of Iberian pigs represents only 5% of the whole Spanish pig production, but it has great significance because it is the source of the most prestigious and highly prized dry-cured products. 4 Although the optimum quality of meat and hams is associated with both the combination of purebred Iberian genotypes and extensive fattening, Iberian pigs are commonly crossbred with Duroc animals in order to improve their efficiency for lean growth.…”

Section: Introductionmentioning

confidence: 99%

DNA tests based on coat colour genes for authentication of the raw material of meat products from Iberian pigs

Fernández¹,

Fabuel²,

Alves³

et al. 2004

J Sci Food Agric

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Traceability in the meat supply chain depends on systems based on electronic identification, but DNA tests would be suitable to audit the genetic origin of some labelled meat products. Differentiated labelling is required for highly prized meat products from purebred Iberian pigs and from animals crossbred with the Duroc breed. A panel of breed specific markers based on polymorphisms found in two coat colour genes was investigated in the present study. The genotyping of the G/C or A/G polymorphisms at nucleotide positions −160 and 727 bp of the melanocortin receptor 1 (MC1R) gene can be indistinctly used to discriminate the Duroc specific allele (MC1R * 4) from all the alleles (MC1R * 3, MC1R * 6 and MC1R * 7) found in the Iberian breed. It allows one to differentiate unambiguously samples from purebred Iberian and 1 2 Duroc crossbred genotypes. The additional genotyping of the A/G polymorphism at position 2462 of the pink-eyed dilution (OCA2) gene, four microsatellites (SW24, SW413, SW874 and SW1057) and nine amplified fragment length polymorphism (AFLP) markers would allow one to detect 1 4 Duroc crossbred genotypes with a probability of exclusion of the pure Iberian origin greater than 0.968. The validation of batches of marketed products may be achieved with more single tests on pooled DNA samples.

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“…Bovine spongiform encephalopathy (BSE) has certainly been the most serious food safety problem of recent years, causing a drastic reduction of beef consumption in all Europe. It was then followed by the dioxin crisis and the avian influenza in the poultry sector [28,29]. Furthermore, the incidence of food borne diseases due to microbial contamination of processed food products has increased in the last decade leading to additional food scares in the buyers [30].…”

Section: Dna Barcoding and The Food Production Chainsmentioning

confidence: 99%

DNA Barcoding as a Molecular Tool to Track Down Mislabeling and Food Piracy

2015

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DNA barcoding is a molecular technology that allows the identification of any biological species by amplifying, sequencing and querying the information from genic and/or intergenic standardized target regions belonging to the extranuclear genomes. Although these sequences represent a small fraction of the total DNA of a cell, both chloroplast and mitochondrial barcodes chosen for identifying plant and animal species, respectively, have shown sufficient nucleotide diversity to assess the taxonomic identity of the vast majority of organisms used in agriculture. Consequently, cpDNA and mtDNA barcoding protocols are being used more and more in the food industry and food supply chains for food labeling, not only to support food safety but also to uncover food piracy in freshly commercialized and technologically processed products. Since the extranuclear genomes are present in many copies within each cell, this technology is being more easily exploited to recover information even in degraded samples or transformed materials deriving from crop varieties and livestock species. The strong standardization that characterizes protocols used worldwide for DNA barcoding makes this technology particularly suitable for routine analyses required by agencies to safeguard food safety and quality. Here we conduct a critical review of the potentials of DNA barcoding for food labeling along with the main findings in the area of food piracy, with particular reference to agrifood and livestock foodstuffs.

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Genomic identification of the breed of an individual or its tissue

Cited by 32 publications

References 6 publications

Comparison of Four Italian Beef Cattle Breeds by Means of Functional Genes

Comparison of Four Italian Beef Cattle Breeds by Means of Functional Genes

DNA tests based on coat colour genes for authentication of the raw material of meat products from Iberian pigs

DNA Barcoding as a Molecular Tool to Track Down Mislabeling and Food Piracy

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